##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        KspI        MaeII       MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TseI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcsI          379  AAATTT               [GA]^AATT[CT]
AocI            5  CCTCAGG              CC^T[ACGT]AGG
ApoI          379  AAATTT               [GA]^AATT[CT]
AvaII         415  GGACC                G^G[AT]CC
BamHI         460  GGATCC               G^GATCC
BanI          312  GGCACC               G^G[CT][GA]CC
BfaI          322  CTAG                 C^TAG
BmyI          311  GGGCAC               G[AGT]GC[ACT]^C
BsaBI         424  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         359  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          122  GTGCAG               GTGCAG(16/14)
BsmAI         116  GTCTC                GTCTC(1/5)
BsmI          218  GAATGC               GAATGC(1/-1)
Bsp1286I      311  GGGCAC               G[AGT]GC[ACT]^C
Bsu36I          5  CCTCAGG              CC^T[ACGT]AGG
Cfr13I        415  GGACC                G^G[ACGT]CC
EarI          289  GAAGAG               CTCTTC(1/4)
Eco81I          5  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       359  CCAAGG               C^C[AT][AT]GG
HaeIII          3  GGCC                 GG^CC
HindIII       261  AAGCTT               A^AGCTT
HinfI         277  GAATC                G^A[ACGT]TC
Ksp632I       289  GAAGAG               CTCTTC(1/4)
MaeI          322  CTAG                 C^TAG
MaeIII        184  GTAAC                ^GT[ACGT]AC
MamI          424  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI          436  GCTTGTATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
Sau96I        415  GGACC                G^G[ACGT]CC
StyI          359  CCAAGG               C^C[AT][AT]GG
TfiI          277  GAATC                G^A[AT]TC
TspRI         120  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BglII         161  AGATCT               A^GATCT
              423  AGATCT              
BstF5I        363  GGATG                GGATG(2/0)
              484  GGATG               
Cac8I         259  GCAAGC               GC[ACGT]^[ACGT]GC
              352  GCAAGC              
DdeI            6  CTCAG                C^T[ACGT]AG
              118  CTCAG               
FokI          363  GGATG                GGATG(9/13)
              484  GGATG               
MnlI            5  CCTC                 CCTC(7/6)
              328  CCTC                
MseI          371  TTAA                 T^TAA
              466  TTAA                
NlaIII         51  CATG                 CATG^
              397  CATG                
NlaIV         312  GGCACC               GG[ACGT]^[ACGT]CC
              460  GGATCC              
Tru9I         371  TTAA                 T^TAA
              466  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        AlwI        BstYI       DpnII       MboI        
MboII       MflI        NdeII       Sau3AI      SfaNI       
Tsp509I     XhoII