Restriction map for
pF1KB3919
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccI AccIII AclI AfaI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BbeI BbrPI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 866 GACGTC GACGT^C
AccII 117 CGCG CG^CG
AocI 1157 CCTCAGG CC^T[ACGT]AGG
AspHI 211 GAGCAC G[AT]GC[AT]^C
AspI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 503 CCCGAG C^[CT]CG[GA]G
BamHI 556 GGATCC G^GATCC
BbsI 105 GAAGAC GAAGAC(2/6)
BciVI 562 GTATCC GTATCC(6/5)
BpuAI 105 GAAGAC GAAGAC(2/6)
BsaI 548 GGTCTC GGTCTC(1/5)
BsiHKAI 211 GAGCAC G[AT]GC[AT]^C
BsoBI 503 CCCGAG C^[CT]CG[GA]G
BspDI 685 ATCGAT AT^CGAT
BspHI 1028 TCATGA T^CATGA
BspLU11I 1003 ACATGT A^CATGT
BssSI 1571 CTCGTG CACGAG(-5/-1)
BstAPI 1336 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 117 CGCG CG^CG
BstXI 612 CCAATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1157 CCTCAGG CC^T[ACGT]AGG
ClaI 685 ATCGAT AT^CGAT
EaeI 473 TGGCCG [CT]^GGCC[GA]
Eco81I 1157 CCTCAGG CC^T[ACGT]AGG
EcoRV 933 GATATC GAT^ATC
EcoT14I 1115 CCTTGG C^C[AT][AT]GG
EcoT22I 790 ATGCAT ATGCA^T
HaeII 165 GGCGCT [GA]GCGC^[CT]
MaeII 867 ACGT A^CGT
MluI 116 ACGCGT A^CGCGT
MslI 1624 CACTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1328 CAGCGG C[AC]G^C[GT]G
MvnI 117 CGCG CG^CG
NsiI 790 ATGCAT ATGCA^T
NspI 1003 ACATGT [GA]CATG^[CT]
PflFI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 90 GGGACCC [GA]G^G[AT]CC[CT]
PstI 356 CTGCAG CTGCA^G
RcaI 1028 TCATGA T^CATGA
SfcI 356 CTGCAG C^T[GA][CT]AG
SfiI 147 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 300 ACTAGT A^CTAGT
StyI 1115 CCTTGG C^C[AT][AT]GG
Tth111I 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 70 GACGCC G[GA]^CG[CT]C
866 GACGTC
AflIII 116 ACGCGT A^C[GA][CT]GT
1003 ACATGT
ApaI 146 GGGCCC GGGCC^C
1162 GGGCCC
BbvCI 24 CCTCAGC CCTCAGC(-5/-2)
1015 GCTGAGG
BcgI 231 GCAAAGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1287 GCAGCTTTCTCG
BglI 148 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
157 GCCATCGAGGC
BsaBI 557 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
729 GATTACTATC
BsaHI 70 GACGCC G[GA]^CG[CT]C
866 GACGTC
BsgI 187 CTGCAC GTGCAG(16/14)
1553 GTGCAG
BsrDI 941 CATTGC GCAATG(2/0)
992 GCAATG
CfoI 166 GCGC GCG^C
594 GCGC
EarI 823 GAAGAG CTCTTC(1/4)
1450 GAAGAG
HgaI 70 GACGC GACGC(5/10)
954 GACGC
HhaI 166 GCGC GCG^C
594 GCGC
Hin1I 70 GACGCC G[GA]^CG[CT]C
866 GACGTC
HinP1I 166 GCGC G^CGC
594 GCGC
Ksp632I 823 GAAGAG CTCTTC(1/4)
1450 GAAGAG
MamI 557 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
729 GATTACTATC
SfaNI 1143 GATGC GCATC(5/9)
1342 GATGC
TfiI 237 GATTC G^A[AT]TC
1074 GAATC
Tsp45I 1574 GTGAC ^GT[GC]AC
1622 GTCAC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
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AciI AcsI AluI AlwI ApoI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SmlI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII