##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvI        BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MroI        MslI        MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TseI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI          271  GCGG                 CCGC(-3/-1)
AlwI           97  GATCC                GGATC(4/5)
BalI          398  TGGCCA               TGG^CCA
BbvCI         431  CCTCAGC              CCTCAGC(-5/-2)
BciVI         168  GGATAC               GTATCC(6/5)
BfaI          337  CTAG                 C^TAG
BsaI          203  GAGACC               GGTCTC(1/5)
BsmAI         203  GAGAC                GTCTC(1/5)
BsmFI         529  GGGAC                GGGAC(10/14)
BspMI          31  GCAGGT               ACCTGC(4/8)
BstF5I        318  GGATG                GGATG(2/0)
CfoI           50  GCGC                 GCG^C
DpnII          97  GATC                 ^GATC
EaeI          398  TGGCCA               [CT]^GGCC[GA]
EarI          184  GAAGAG               CTCTTC(1/4)
FokI          318  GGATG                GGATG(9/13)
HaeIII        399  GGCC                 GG^CC
HhaI           50  GCGC                 GCG^C
HinP1I         50  GCGC                 G^CGC
Ksp632I       184  GAAGAG               CTCTTC(1/4)
MaeI          337  CTAG                 C^TAG
MaeIII        407  GTTAC                ^GT[ACGT]AC
MboI           97  GATC                 ^GATC
MluNI         398  TGGCCA               TGG^CCA
MscI          398  TGGCCA               TGG^CCA
MspA1I        173  CAGCTG               C[AC]G^C[GT]G
MwoI          294  GCAGATTAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          97  GATC                 ^GATC
PflMI         390  CCACGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         173  CAGCTG               CAG^CTG
Sau3AI         97  GATC                 ^GATC
SfaNI         460  GATGC                GCATC(5/9)
SfcI          285  CTACAG               C^T[GA][CT]AG
Van91I        390  CCACGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI           65  AGCT                 AG^CT
              174  AGCT                
BsiYI         282  CCTCTACAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              390  CCACGAACTGG         
BslI          282  CCTCTACAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              390  CCACGAACTGG         
HphI          142  GGTGA                GGTGA(8/7)
              561  GGTGA               
MboII         184  GAAGA                GAAGA(8/7)
              457  GAAGA               
MseI          278  TTAA                 T^TAA
              299  TTAA                
PleI          313  GACTC                GAGTC(4/5)
              531  GACTC               
TfiI          112  GATTC                G^A[AT]TC
              414  GATTC               
Tru9I         278  TTAA                 T^TAA
              299  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AfaI        BsrI        DdeI        HinfI       MnlI        
NlaIII      RsaI        TspRI