Restriction map for
pF1KB4080
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BpmI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 613 GGTACC G^GTACC
AccI 674 GTATAC GT^[AC][GT]AC
AciI 801 GCGG CCGC(-3/-1)
AlwI 483 GGATC GGATC(4/5)
Aor51HI 302 AGCGCT AGC^GCT
AseI 589 ATTAAT AT^TAAT
AsnI 589 ATTAAT AT^TAAT
Asp700I 765 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 613 GGTACC G^GTACC
AvaI 839 CTCGAG C^[CT]CG[GA]G
BanI 613 GGTACC G^G[CT][GA]CC
BanII 317 GAGCCC G[GA]GC[CT]^C
BbsI 474 GAAGAC GAAGAC(2/6)
BciVI 150 GGATAC GTATCC(6/5)
BglII 956 AGATCT A^GATCT
BlpI 556 GCTCAGC GC^T[ACGT]AGC
BmyI 317 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 556 GCTCAGC GC^T[ACGT]AGC
BpuAI 474 GAAGAC GAAGAC(2/6)
BsgI 427 CTGCAC GTGCAG(16/14)
BsiYI 896 CCAAAGACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 896 CCAAAGACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 869 GAGAC GTCTC(1/5)
BsmI 644 GCATTC GAATGC(1/-1)
BsoBI 839 CTCGAG C^[CT]CG[GA]G
Bsp1286I 317 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 137 ACATGT A^CATGT
BssKI 944 CCTGG ^CC[ACGT]GG
Bst1107I 674 GTATAC GTA^TAC
BstNI 944 CCTGG CC^[AT]GG
BstYI 956 AGATCT [GA]^GATC[CT]
BstZ17I 674 GTATAC GTA^TAC
Cac8I 791 GCTTGC GC[ACGT]^[ACGT]GC
CelII 556 GCTCAGC GC^T[ACGT]AGC
CfoI 303 GCGC GCG^C
Eco47III 302 AGCGCT AGC^GCT
EcoRII 944 CCTGG ^CC[AT]GG
EcoRV 184 GATATC GAT^ATC
HaeII 302 AGCGCT [GA]GCGC^[CT]
HaeIII 363 GGCC GG^CC
HapII 148 CCGG C^CGG
HhaI 303 GCGC GCG^C
HinP1I 303 GCGC G^CGC
HindIII 195 AAGCTT A^AGCTT
HpaII 148 CCGG C^CGG
HphI 287 TCACC GGTGA(8/7)
KpnI 613 GGTACC GGTAC^C
MaeII 85 ACGT A^CGT
MaeIII 344 GTTAC ^GT[ACGT]AC
MflI 956 AGATCT [GA]^GATC[CT]
MslI 366 CATCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 148 CCGG C^CGG
MvaI 944 CCTGG CC^[AT]GG
PaeR7I 839 CTCGAG C^TCGAG
PleI 871 GACTC GAGTC(4/5)
PshBI 589 ATTAAT AT^TAAT
PvuII 927 CAGCTG CAG^CTG
ScaI 995 AGTACT AGT^ACT
ScrFI 944 CCTGG CC^[ACGT]GG
XhoI 839 CTCGAG C^TCGAG
XhoII 956 AGATCT [GA]^GATC[CT]
XmnI 765 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 83 ACACGT A^C[GA][CT]GT
137 ACATGT
BbvI 300 GCAGC GCAGC(8/12)
721 GCTGC
BfaI 523 CTAG C^TAG
713 CTAG
BstXI 376 CCACCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
774 CCAAATTCATGG
DpnII 484 GATC ^GATC
957 GATC
EcoRI 503 GAATTC G^AATTC
512 GAATTC
MaeI 523 CTAG C^TAG
713 CTAG
MboI 484 GATC ^GATC
957 GATC
MspA1I 799 CAGCGG C[AC]G^C[GT]G
927 CAGCTG
MwoI 712 GCTAGAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
795 GCAACAGCGGC
NdeII 484 GATC ^GATC
957 GATC
NspI 137 ACATGT [GA]CATG^[CT]
881 ACATGC
Sau3AI 484 GATC ^GATC
957 GATC
TaqI 188 TCGA T^CGA
840 TCGA
TfiI 12 GAATC G^A[AT]TC
282 GAATC
TseI 300 GCAGC G^C[AT]GC
721 GCTGC
TthHB8I 188 TCGA T^CGA
840 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsrI
BstF5I DdeI Fnu4HI FokI HinfI
ItaI MboII MnlI MseI NlaIII
NlaIV RsaI SfaNI SmlI Tru9I
Tsp509I TspRI