Restriction map for
pF1KB4098
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbrPI BbvCI BcgI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BsaJI BsaWI BseAI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CpoI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT14I FseI HgaI HpaI KpnI
KspI MaeI MaeII MluI MroI
NcoI NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 76 enzymes) #
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AccII 83 CGCG CG^CG
AcyI 46 GGCGCC G[GA]^CG[CT]C
Aor51HI 28 AGCGCT AGC^GCT
Asp700I 1117 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1164 GAGCTC G[AT]GC[AT]^C
AspI 442 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 62 CCCGAG C^[CT]CG[GA]G
AvaII 609 GGTCC G^G[AT]CC
AviII 23 TGCGCA TGC^GCA
BalI 1226 TGGCCA TGG^CCA
BanII 1164 GAGCTC G[GA]GC[CT]^C
BbeI 46 GGCGCC GGCGC^C
BciVI 1187 GTATCC GTATCC(6/5)
BclI 645 TGATCA T^GATCA
BcnI 965 CCCGG CC^[GC]GG
BglII 1038 AGATCT A^GATCT
BpmI 665 CTCCAG CTGGAG(16/14)
BsaBI 1039 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
BseRI 881 GAGGAG GAGGAG(10/8)
BsgI 938 CTGCAC GTGCAG(16/14)
BsiHKAI 1164 GAGCTC G[AT]GC[AT]^C
BsmAI 189 GAGAC GTCTC(1/5)
BsoBI 62 CCCGAG C^[CT]CG[GA]G
Bsp1407I 433 TGTACA T^GTACA
BspDI 479 ATCGAT AT^CGAT
BsrDI 775 GCAATG GCAATG(2/0)
BsrFI 49 GCCGGC [GA]^CCGG[CT]
BsrGI 433 TGTACA T^GTACA
BstBI 368 TTCGAA TT^CGAA
BstUI 83 CGCG CG^CG
BstYI 1038 AGATCT [GA]^GATC[CT]
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
ClaI 479 ATCGAT AT^CGAT
DraIII 718 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1226 TGGCCA [CT]^GGCC[GA]
EarI 246 CTCTTC CTCTTC(1/4)
Eco47III 28 AGCGCT AGC^GCT
EcoRI 301 GAATTC G^AATTC
EcoRV 217 GATATC GAT^ATC
EcoT22I 140 ATGCAT ATGCA^T
FbaI 645 TGATCA T^GATCA
FspI 23 TGCGCA TGC^GCA
Hin1I 46 GGCGCC G[GA]^CG[CT]C
HincII 439 GTTGAC GT[CT]^[GA]AC
HindII 439 GTTGAC GT[CT]^[GA]AC
HindIII 676 AAGCTT A^AGCTT
KasI 46 GGCGCC G^GCGCC
Ksp632I 246 CTCTTC CTCTTC(1/4)
MamI 1039 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 256 CAATTG C^AATTG
MflI 1038 AGATCT [GA]^GATC[CT]
MluNI 1226 TGGCCA TGG^CCA
MscI 1226 TGGCCA TGG^CCA
MslI 379 CACCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 100 CCGCTG C[AC]G^C[GT]G
MunI 256 CAATTG C^AATTG
MvnI 83 CGCG CG^CG
NaeI 49 GCCGGC GCC^GGC
NarI 46 GGCGCC GG^CGCC
NciI 965 CCCGG CC^[GC]GG
NdeI 748 CATATG CA^TATG
NgoMIV 49 GCCGGC G^CCGGC
NsiI 140 ATGCAT ATGCA^T
NspI 142 GCATGT [GA]CATG^[CT]
NspV 368 TTCGAA TT^CGAA
PflFI 442 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1164 GAGCTC GAGCT^C
ScaI 1025 AGTACT AGT^ACT
SfoI 46 GGCGCC GGC^GCC
SfuI 368 TTCGAA TT^CGAA
SspBI 433 TGTACA T^GTACA
SspI 467 AATATT AAT^ATT
Tth111I 442 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1038 AGATCT [GA]^GATC[CT]
XmnI 1117 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 301 GAATTC [GA]^AATT[CT]
697 AAATTT
AfaI 434 GTAC GT^AC
1026 GTAC
AlwI 583 GATCC GGATC(4/5)
477 GGATC
AlwNI 115 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
280 CAGAGCCTG
ApoI 301 GAATTC [GA]^AATT[CT]
697 AAATTT
BanI 46 GGCGCC G^G[CT][GA]CC
57 GGTGCC
BbsI 853 GAAGAC GAAGAC(2/6)
1101 GTCTTC
BmyI 58 GTGCCC G[AGT]GC[ACT]^C
1164 GAGCTC
BpuAI 853 GAAGAC GAAGAC(2/6)
1101 GTCTTC
BsmFI 96 GTCCC GGGAC(10/14)
391 GTCCC
BsmI 771 GAATGC GAATGC(1/-1)
969 GAATGC
Bsp1286I 58 GTGCCC G[AGT]GC[ACT]^C
1164 GAGCTC
BstF5I 509 GGATG GGATG(2/0)
635 GGATG
Cfr13I 9 GGCCC G^G[ACGT]CC
609 GGTCC
FokI 509 GGATG GGATG(9/13)
635 GGATG
HaeII 28 AGCGCT [GA]GCGC^[CT]
46 GGCGCC
HapII 50 CCGG C^CGG
966 CCGG
HpaII 50 CCGG C^CGG
966 CCGG
HphI 449 TCACC GGTGA(8/7)
738 TCACC
MspI 50 CCGG C^CGG
966 CCGG
RsaI 434 GTAC GT^AC
1026 GTAC
Sau96I 9 GGCCC G^G[ACGT]CC
609 GGTCC
SfcI 87 CTACAG C^T[GA][CT]AG
1112 CTATAG
TaqI 369 TCGA T^CGA
480 TCGA
TfiI 410 GAATC G^A[AT]TC
1044 GAATC
Tsp45I 448 GTCAC ^GT[GC]AC
542 GTGAC
TthHB8I 369 TCGA T^CGA
480 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BsrI BssKI BstNI Cac8I CfoI
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI SfaNI Tru9I
TseI Tsp509I TspRI