Restriction map for
pF1KB4099
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 214 AACGTT AA^CGTT
AcsI 192 AAATTT [GA]^AATT[CT]
AhdI 91 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 845 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 192 AAATTT [GA]^AATT[CT]
AspEI 91 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 478 GTGCTC G[AT]GC[AT]^C
AvaI 1265 CTCGAG C^[CT]CG[GA]G
BanI 415 GGTGCC G^G[CT][GA]CC
BcnI 1239 CCGGG CC^[GC]GG
BmyI 478 GTGCTC G[AGT]GC[ACT]^C
BsaI 930 GGTCTC GGTCTC(1/5)
BsiHKAI 478 GTGCTC G[AT]GC[AT]^C
BsmBI 1020 CGTCTC CGTCTC(1/5)
BsmFI 515 GTCCC GGGAC(10/14)
BsoBI 1265 CTCGAG C^[CT]CG[GA]G
Bsp1286I 478 GTGCTC G[AGT]GC[ACT]^C
BsrFI 5 ACCGGC [GA]^CCGG[CT]
BssSI 1094 CTCGTG CACGAG(-5/-1)
BstAPI 844 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 462 TTCGAA TT^CGAA
CfoI 1047 GCGC GCG^C
Cfr10I 5 ACCGGC [GA]^CCGG[CT]
DraI 196 TTTAAA TTT^AAA
DraII 201 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 91 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 201 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1260 GATATC GAT^ATC
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
NciI 1239 CCGGG CC^[GC]GG
NspV 462 TTCGAA TT^CGAA
PaeR7I 1265 CTCGAG C^TCGAG
PflMI 1201 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 201 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 214 AACGTT AA^CGTT
SfcI 483 CTACAG C^T[GA][CT]AG
SfuI 462 TTCGAA TT^CGAA
SmlI 1265 CTCGAG C^T[CT][GA]AG
Van91I 1201 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1265 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BbsI 1058 GAAGAC GAAGAC(2/6)
1084 GAAGAC
BfaI 13 CTAG C^TAG
370 CTAG
BpuAI 1058 GAAGAC GAAGAC(2/6)
1084 GAAGAC
BsaBI 780 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
948 GATTGACATC
BspMI 724 ACCTGC ACCTGC(4/8)
1176 ACCTGC
Cac8I 318 GCTGGC GC[ACGT]^[ACGT]GC
794 GCCTGC
DdeI 48 CTTAG C^T[ACGT]AG
292 CTGAG
DsaI 266 CCATGG C^C[GA][CT]GG
518 CCATGG
EarI 673 GAAGAG CTCTTC(1/4)
1065 GAAGAG
EcoT14I 266 CCATGG C^C[AT][AT]GG
518 CCATGG
HapII 6 CCGG C^CGG
1239 CCGG
HpaII 6 CCGG C^CGG
1239 CCGG
Ksp632I 673 GAAGAG CTCTTC(1/4)
1065 GAAGAG
MaeI 13 CTAG C^TAG
370 CTAG
MaeII 215 ACGT A^CGT
1088 ACGT
MamI 780 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
948 GATTGACATC
MslI 954 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1336 CATGAAGATG
MspI 6 CCGG C^CGG
1239 CCGG
NcoI 266 CCATGG C^CATGG
518 CCATGG
PvuII 565 CAGCTG CAG^CTG
1160 CAGCTG
SspI 226 AATATT AAT^ATT
283 AATATT
StyI 266 CCATGG C^C[AT][AT]GG
518 CCATGG
TaqI 463 TCGA T^CGA
1266 TCGA
TfiI 182 GAATC G^A[AT]TC
310 GATTC
TthHB8I 463 TCGA T^CGA
1266 TCGA
XcmI 854 CCAGGGTCGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1143 CCAAGTGAAAGATGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BpmI BsaJI BsiYI BslI
BsmAI Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI Tru9I
TseI Tsp45I Tsp509I TspRI