Restriction map for
pF1KB4161
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BstBI BstUI
BstXI Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FauI FbaI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MroI MslI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 213 GTATAC GT^[AC][GT]AC
AciI 1126 GCGG CCGC(-3/-1)
AflII 1186 CTTAAG C^TTAAG
AspHI 1242 GAGCTC G[AT]GC[AT]^C
BalI 363 TGGCCA TGG^CCA
BbsI 1196 GTCTTC GAAGAC(2/6)
BbvCI 382 GCTGAGG CCTCAGC(-5/-2)
BciVI 779 GTATCC GTATCC(6/5)
BfrI 1186 CTTAAG C^TTAAG
BpmI 1070 CTGGAG CTGGAG(16/14)
BpuAI 1196 GTCTTC GAAGAC(2/6)
BsgI 1079 GTGCAG GTGCAG(16/14)
BsiHKAI 1242 GAGCTC G[AT]GC[AT]^C
BsmBI 1105 GAGACG CGTCTC(1/5)
BspHI 1019 TCATGA T^CATGA
BspMI 858 ACCTGC ACCTGC(4/8)
BsrDI 1122 CATTGC GCAATG(2/0)
BssSI 638 CTCGTG CACGAG(-5/-1)
Bst1107I 213 GTATAC GTA^TAC
BstAPI 792 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1277 GGTCACC G^GT[ACGT]ACC
BstF5I 852 CATCC GGATG(2/0)
BstPI 1277 GGTCACC G^GT[ACGT]ACC
BstYI 1317 GGATCT [GA]^GATC[CT]
BstZ17I 213 GTATAC GTA^TAC
EarI 1245 CTCTTC CTCTTC(1/4)
EcoO65I 1277 GGTCACC G^GT[ACGT]ACC
EcoRV 1015 GATATC GAT^ATC
EcoT14I 957 CCATGG C^C[AT][AT]GG
FokI 852 CATCC GGATG(9/13)
FseI 473 GGCCGGCC GGCCGG^CC
HindIII 750 AAGCTT A^AGCTT
Ksp632I 1245 CTCTTC CTCTTC(1/4)
MfeI 1100 CAATTG C^AATTG
MflI 1317 GGATCT [GA]^GATC[CT]
MluNI 363 TGGCCA TGG^CCA
MscI 363 TGGCCA TGG^CCA
MspA1I 289 CAGCTG C[AC]G^C[GT]G
MunI 1100 CAATTG C^AATTG
MwoI 792 GCATCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 474 GCCGGC GCC^GGC
NcoI 957 CCATGG C^CATGG
NgoMIV 474 GCCGGC G^CCGGC
PvuII 289 CAGCTG CAG^CTG
RcaI 1019 TCATGA T^CATGA
SacI 1242 GAGCTC GAGCT^C
SapI 1244 GCTCTTC GCTCTTC(1/4)
SfaNI 792 GCATC GCATC(5/9)
StuI 935 AGGCCT AGG^CCT
StyI 957 CCATGG C^C[AT][AT]GG
XhoII 1317 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 340 AAATTT [GA]^AATT[CT]
1006 GAATTT
AfaI 625 GTAC GT^AC
835 GTAC
AlwI 294 GATCC GGATC(4/5)
1317 GGATC
ApaI 563 GGGCCC GGGCC^C
975 GGGCCC
ApoI 340 AAATTT [GA]^AATT[CT]
1006 GAATTT
BfaI 581 CTAG C^TAG
1283 CTAG
BsmAI 1105 GAGAC GTCTC(1/5)
1380 GAGAC
Cac8I 227 GCCTGC GC[ACGT]^[ACGT]GC
474 GCCGGC
DsaI 559 CCACGG C^C[GA][CT]GG
957 CCATGG
EaeI 363 TGGCCA [CT]^GGCC[GA]
476 CGGCCA
HgaI 1096 GACGC GACGC(5/10)
1107 GACGC
HphI 867 TCACC GGTGA(8/7)
1279 TCACC
MaeI 581 CTAG C^TAG
1283 CTAG
MaeII 467 ACGT A^CGT
522 ACGT
MseI 449 TTAA T^TAA
1187 TTAA
PleI 317 GAGTC GAGTC(4/5)
536 GAGTC
PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT]
758 GGGTCCC
RsaI 625 GTAC GT^AC
835 GTAC
SmlI 747 CTCAAG C^T[CT][GA]AG
1186 CTTAAG
TaqI 354 TCGA T^CGA
813 TCGA
Tru9I 449 TTAA T^TAA
1187 TTAA
TthHB8I 354 TCGA T^CGA
813 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AluI AlwNI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MspI MvaI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfcI SmaI TfiI TseI
Tsp45I Tsp509I TspRI XmaCI XmaI