Restriction map for
pF1KB4166
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbsI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 22 GTAGAC GT^[AC][GT]AC
AccII 703 CGCG CG^CG
AflIII 329 ACATGT A^C[GA][CT]GT
AlwI 345 GGATC GGATC(4/5)
AlwNI 983 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 358 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 291 TGGCCA TGG^CCA
BbeI 424 GGCGCC GGCGC^C
BbrPI 277 CACGTG CAC^GTG
BciVI 937 GGATAC GTATCC(6/5)
BfaI 953 CTAG C^TAG
BsaAI 277 CACGTG [CT]AC^GT[GA]
BseRI 847 CTCCTC GAGGAG(10/8)
BspLU11I 329 ACATGT A^CATGT
BsrI 521 ACTGG ACTGG(1/-1)
BstAPI 793 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 703 CGCG CG^CG
EarI 226 CTCTTC CTCTTC(1/4)
EcoNI 27 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 424 GGCGCC G^GCGCC
Ksp632I 226 CTCTTC CTCTTC(1/4)
MaeI 953 CTAG C^TAG
MluNI 291 TGGCCA TGG^CCA
MscI 291 TGGCCA TGG^CCA
MspA1I 349 CAGCTG C[AC]G^C[GT]G
MvnI 703 CGCG CG^CG
NarI 424 GGCGCC GG^CGCC
NheI 952 GCTAGC G^CTAGC
PmaCI 277 CACGTG CAC^GTG
PmlI 277 CACGTG CAC^GTG
PvuII 349 CAGCTG CAG^CTG
ScaI 917 AGTACT AGT^ACT
SfoI 424 GGCGCC GGC^GCC
SmlI 850 CTCAAG C^T[CT][GA]AG
StuI 908 AGGCCT AGG^CCT
Tsp509I 824 AATT ^AATT
XcmI 341 CCATGGATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 358 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 283 GACGCC G[GA]^CG[CT]C
424 GGCGCC
ApaI 1106 GGGCCC GGGCC^C
1156 GGGCCC
AvaI 5 CTCGGG C^[CT]CG[GA]G
1131 CTCGGG
BanII 1106 GGGCCC G[GA]GC[CT]^C
1156 GGGCCC
BbvCI 31 GCTGAGG CCTCAGC(-5/-2)
904 GCTGAGG
BglII 536 AGATCT A^GATCT
881 AGATCT
BpmI 368 CTGGAG CTGGAG(16/14)
709 CTGGAG
BsaHI 283 GACGCC G[GA]^CG[CT]C
424 GGCGCC
BsaI 61 GAGACC GGTCTC(1/5)
1173 GGTCTC
BsgI 670 CTGCAC GTGCAG(16/14)
925 GTGCAG
BsoBI 5 CTCGGG C^[CT]CG[GA]G
1131 CTCGGG
BspMI 211 ACCTGC ACCTGC(4/8)
668 ACCTGC
BstXI 579 CCACCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1160 CCAAGTCCATGG
BstYI 536 AGATCT [GA]^GATC[CT]
881 AGATCT
DraII 473 AGGGCCT [GA]G^G[ACGT]CC[CT]
1105 GGGGCCC
DraIII 138 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
640 CACATTGTG
EaeI 291 TGGCCA [CT]^GGCC[GA]
893 CGGCCA
EcoO109I 473 AGGGCCT [GA]G^G[ACGT]CC[CT]
1105 GGGGCCC
HaeII 424 GGCGCC [GA]GCGC^[CT]
1150 AGCGCC
HgaI 283 GACGC GACGC(5/10)
998 GCGTC
Hin1I 283 GACGCC G[GA]^CG[CT]C
424 GGCGCC
HinfI 150 GACTC G^A[ACGT]TC
371 GAGTC
MboII 595 GAAGA GAAGA(8/7)
227 TCTTC
MflI 536 AGATCT [GA]^GATC[CT]
881 AGATCT
MslI 192 CATCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
567 CATGGTGGTG
NaeI 698 GCCGGC GCC^GGC
891 GCCGGC
NcoI 341 CCATGG C^CATGG
1166 CCATGG
NgoMIV 698 GCCGGC G^CCGGC
891 GCCGGC
PleI 150 GACTC GAGTC(4/5)
371 GAGTC
PstI 751 CTGCAG CTGCA^G
791 CTGCAG
SfcI 751 CTGCAG C^T[GA][CT]AG
791 CTGCAG
TaqI 110 TCGA T^CGA
441 TCGA
TspRI 754 CAGTG CA[GC]TG[ACGT][ACGT]^
794 CAGTG
TthHB8I 110 TCGA T^CGA
441 TCGA
XhoII 536 AGATCT [GA]^GATC[CT]
881 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I