Restriction map for
pF1KB4220
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 617 GTAGAC GT^[AC][GT]AC
AccIII 428 TCCGGA T^CCGGA
AflII 109 CTTAAG C^TTAAG
AlwNI 568 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 208 CCTGAGG CC^T[ACGT]AGG
ApaI 899 GGGCCC GGGCC^C
AvaI 857 CTCGGG C^[CT]CG[GA]G
BalI 702 TGGCCA TGG^CCA
BamHI 892 GGATCC G^GATCC
BanI 665 GGCACC G^G[CT][GA]CC
BbsI 841 GTCTTC GAAGAC(2/6)
BcnI 303 CCGGG CC^[GC]GG
BfrI 109 CTTAAG C^TTAAG
BpmI 907 CTGGAG CTGGAG(16/14)
BpuAI 841 GTCTTC GAAGAC(2/6)
BsaBI 988 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 292 GAGACC GGTCTC(1/5)
BsaWI 428 TCCGGA [AT]^CCGG[AT]
BseAI 428 TCCGGA T^CCGGA
BsgI 606 GTGCAG GTGCAG(16/14)
BsmI 586 GCATTC GAATGC(1/-1)
BsoBI 857 CTCGGG C^[CT]CG[GA]G
BspEI 428 TCCGGA T^CCGGA
BsrDI 1030 GCAATG GCAATG(2/0)
BstEII 778 GGTAACC G^GT[ACGT]ACC
BstPI 778 GGTAACC G^GT[ACGT]ACC
BstYI 892 GGATCC [GA]^GATC[CT]
Bsu36I 208 CCTGAGG CC^T[ACGT]AGG
CfoI 300 GCGC GCG^C
DraIII 188 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 413 GACTTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 702 TGGCCA [CT]^GGCC[GA]
EarI 1018 GAAGAG CTCTTC(1/4)
Eco81I 208 CCTGAGG CC^T[ACGT]AGG
EcoO65I 778 GGTAACC G^GT[ACGT]ACC
EcoT14I 474 CCAAGG C^C[AT][AT]GG
HhaI 300 GCGC GCG^C
HinP1I 300 GCGC G^CGC
HindIII 202 AAGCTT A^AGCTT
Ksp632I 1018 GAAGAG CTCTTC(1/4)
MaeII 824 ACGT A^CGT
MamI 988 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 892 GGATCC [GA]^GATC[CT]
MluNI 702 TGGCCA TGG^CCA
MroI 428 TCCGGA T^CCGGA
MscI 702 TGGCCA TGG^CCA
NciI 303 CCGGG CC^[GC]GG
PleI 611 GAGTC GAGTC(4/5)
PpuMI 86 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 834 GAGCTC GAGCT^C
SfcI 140 CTGTAG C^T[GA][CT]AG
SmlI 109 CTTAAG C^T[CT][GA]AG
StyI 474 CCAAGG C^C[AT][AT]GG
Tsp45I 691 GTGAC ^GT[GC]AC
Tsp509I 389 AATT ^AATT
TspRI 190 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 892 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 343 CCGC CCGC(-3/-1)
654 CCGC
AfaI 176 GTAC GT^AC
503 GTAC
BanII 834 GAGCTC G[GA]GC[CT]^C
899 GGGCCC
BfaI 925 CTAG C^TAG
938 CTAG
BsmAI 292 GAGAC GTCTC(1/5)
3 GTCTC
BsmFI 183 GGGAC GGGAC(10/14)
88 GTCCC
EcoNI 403 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
873 CCTGGTCAAGG
HapII 303 CCGG C^CGG
429 CCGG
HpaII 303 CCGG C^CGG
429 CCGG
MaeI 925 CTAG C^TAG
938 CTAG
MslI 819 CATAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
913 CACCCTGATG
MspA1I 441 CAGCTG C[AC]G^C[GT]G
904 CAGCTG
MspI 303 CCGG C^CGG
429 CCGG
PflMI 357 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
567 CCAGACCCTGG
PvuII 441 CAGCTG CAG^CTG
904 CAGCTG
RsaI 176 GTAC GT^AC
503 GTAC
Van91I 357 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
567 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AlwI AspHI AvaII BbvI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI