Restriction map for
pF1KB4278
##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AflIII AgeI AhdI AlwNI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BglI BlnI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HaeIII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MscI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 67 GTCTAC GT^[AC][GT]AC
AccIII 377 TCCGGA T^CCGGA
AcsI 674 AAATTC [GA]^AATT[CT]
AfaI 398 GTAC GT^AC
AflII 793 CTTAAG C^TTAAG
Alw44I 981 GTGCAC G^TGCAC
AocI 880 CCTGAGG CC^T[ACGT]AGG
ApaLI 981 GTGCAC G^TGCAC
ApoI 674 AAATTC [GA]^AATT[CT]
AseI 706 ATTAAT AT^TAAT
AsnI 706 ATTAAT AT^TAAT
AvaII 42 GGTCC G^G[AT]CC
BbsI 343 GAAGAC GAAGAC(2/6)
BclI 972 TGATCA T^GATCA
BcnI 132 CCCGG CC^[GC]GG
BfrI 793 CTTAAG C^TTAAG
BglII 789 AGATCT A^GATCT
BlpI 559 GCTCAGC GC^T[ACGT]AGC
BpmI 729 CTGGAG CTGGAG(16/14)
Bpu1102I 559 GCTCAGC GC^T[ACGT]AGC
BpuAI 343 GAAGAC GAAGAC(2/6)
BsaJI 131 CCCCGG C^C[ACGT][ACGT]GG
BsaWI 377 TCCGGA [AT]^CCGG[AT]
BseAI 377 TCCGGA T^CCGGA
BsiYI 880 CCTGAGGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 880 CCTGAGGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 950 GTCTC GTCTC(1/5)
BspEI 377 TCCGGA T^CCGGA
BspMI 28 GCAGGT ACCTGC(4/8)
Bsu36I 880 CCTGAGG CC^T[ACGT]AGG
CelII 559 GCTCAGC GC^T[ACGT]AGC
CfoI 822 GCGC GCG^C
Cfr13I 42 GGTCC G^G[ACGT]CC
DraIII 147 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 82 GAAGAG CTCTTC(1/4)
Eco81I 880 CCTGAGG CC^T[ACGT]AGG
FbaI 972 TGATCA T^GATCA
HhaI 822 GCGC GCG^C
HinP1I 822 GCGC G^CGC
HindIII 569 AAGCTT A^AGCTT
HphI 276 GGTGA GGTGA(8/7)
Ksp632I 82 GAAGAG CTCTTC(1/4)
MaeII 65 ACGT A^CGT
MroI 377 TCCGGA T^CCGGA
MspA1I 562 CAGCTG C[AC]G^C[GT]G
MwoI 463 GCTACTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 132 CCCGG CC^[GC]GG
NlaIV 41 GGGTCC GG[ACGT]^[ACGT]CC
PaeR7I 98 CTCGAG C^TCGAG
PshBI 706 ATTAAT AT^TAAT
PvuII 562 CAGCTG CAG^CTG
RsaI 398 GTAC GT^AC
Sau96I 42 GGTCC G^G[ACGT]CC
SfcI 852 CTATAG C^T[GA][CT]AG
TaqI 99 TCGA T^CGA
TfiI 89 GATTC G^A[AT]TC
TthHB8I 99 TCGA T^CGA
XhoI 98 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 19 GAGCAC G[AT]GC[AT]^C
981 GTGCAC
AvaI 52 CTCGGG C^[CT]CG[GA]G
98 CTCGAG
BmyI 19 GAGCAC G[AGT]GC[ACT]^C
981 GTGCAC
BseRI 962 CTCCTC GAGGAG(10/8)
943 GAGGAG
BsiHKAI 19 GAGCAC G[AT]GC[AT]^C
981 GTGCAC
BsoBI 52 CTCGGG C^[CT]CG[GA]G
98 CTCGAG
Bsp1286I 19 GAGCAC G[AGT]GC[ACT]^C
981 GTGCAC
BspHI 33 TCATGA T^CATGA
366 TCATGA
BstF5I 359 CATCC GGATG(2/0)
419 GGATG
BstYI 211 GGATCT [GA]^GATC[CT]
789 AGATCT
Cac8I 321 GCTGGC GC[ACGT]^[ACGT]GC
571 GCTTGC
FokI 359 CATCC GGATG(9/13)
419 GGATG
HapII 133 CCGG C^CGG
378 CCGG
HpaII 133 CCGG C^CGG
378 CCGG
MflI 211 GGATCT [GA]^GATC[CT]
789 AGATCT
MspI 133 CCGG C^CGG
378 CCGG
NlaIII 34 CATG CATG^
367 CATG
RcaI 33 TCATGA T^CATGA
366 TCATGA
SmlI 98 CTCGAG C^T[CT][GA]AG
793 CTTAAG
SspI 244 AATATT AAT^ATT
352 AATATT
XhoII 211 GGATCT [GA]^GATC[CT]
789 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AlwI BbvI BfaI BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HinfI ItaI MaeI MboI
MboII MnlI MseI MvaI NdeII
PleI Sau3AI ScrFI Tru9I TseI
Tsp509I TspRI