################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BspDI BspEI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI Ksp632I MaeI MamI MboII MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SapI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI SspI SwaI Tru9I Tsp509I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Asp700I 259 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 722 CTCGAG C^[CT]CG[GA]G AviII 345 TGCGCA TGC^GCA BbvCI 1433 CCTCAGC CCTCAGC(-5/-2) BsaI 725 GAGACC GGTCTC(1/5) BsmBI 1318 GAGACG CGTCTC(1/5) BsmI 263 GCATTC GAATGC(1/-1) BsoBI 722 CTCGAG C^[CT]CG[GA]G Bsp1407I 701 TGTACA T^GTACA BspHI 977 TCATGA T^CATGA BsrBI 546 GAGCGG CCGCTC(-3/-3) BsrFI 340 GCCGGT [GA]^CCGG[CT] BsrGI 701 TGTACA T^GTACA BstXI 784 CCATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 340 GCCGGT [GA]^CCGG[CT] EaeI 906 TGGCCG [CT]^GGCC[GA] EcoNI 759 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 588 CCATGG C^C[AT][AT]GG FspI 345 TGCGCA TGC^GCA HaeII 215 AGCGCC [GA]GCGC^[CT] HincII 1240 GTCAAC GT[CT]^[GA]AC HindII 1240 GTCAAC GT[CT]^[GA]AC KspI 1202 CCGCGG CCGC^GG NcoI 588 CCATGG C^CATGG NdeI 1229 CATATG CA^TATG NspI 572 ACATGC [GA]CATG^[CT] PaeR7I 722 CTCGAG C^TCGAG PflMI 605 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 672 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 977 TCATGA T^CATGA SacII 1202 CCGCGG CCGC^GG SbfI 528 CCTGCAGG CCTGCA^GG SgrAI 339 CGCCGGTG C[GA]^CCGG[CT]G SmlI 722 CTCGAG C^T[CT][GA]AG Sse8387I 528 CCTGCAGG CCTGCA^GG SspBI 701 TGTACA T^GTACA StyI 588 CCATGG C^C[AT][AT]GG TfiI 1223 GAATC G^A[AT]TC Van91I 605 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 605 CCAAGTTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 722 CTCGAG C^TCGAG XmnI 259 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 1203 CGCG CG^CG 1270 CGCG AcyI 592 GGCGTC G[GA]^CG[CT]C 1069 GGCGTC AocI 268 CCTGAGG CC^T[ACGT]AGG 1392 CCTGAGG ApaI 205 GGGCCC GGGCC^C 508 GGGCCC BcnI 797 CCCGG CC^[GC]GG 953 CCCGG BglII 737 AGATCT A^GATCT 839 AGATCT BsaHI 592 GGCGTC G[GA]^CG[CT]C 1069 GGCGTC BseRI 43 GAGGAG GAGGAG(10/8) 433 GAGGAG BspMI 527 ACCTGC ACCTGC(4/8) 986 ACCTGC BstAPI 531 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1451 GCAACGCCTGC BstUI 1203 CGCG CG^CG 1270 CGCG BstYI 737 AGATCT [GA]^GATC[CT] 839 AGATCT Bsu36I 268 CCTGAGG CC^T[ACGT]AGG 1392 CCTGAGG DpnII 738 GATC ^GATC 840 GATC Eco81I 268 CCTGAGG CC^T[ACGT]AGG 1392 CCTGAGG Hin1I 592 GGCGTC G[GA]^CG[CT]C 1069 GGCGTC MboI 738 GATC ^GATC 840 GATC MflI 737 AGATCT [GA]^GATC[CT] 839 AGATCT MvnI 1203 CGCG CG^CG 1270 CGCG NciI 797 CCCGG CC^[GC]GG 953 CCCGG NdeII 738 GATC ^GATC 840 GATC PleI 162 GACTC GAGTC(4/5) 1405 GACTC PpuMI 113 GGGTCCT [GA]G^G[AT]CC[CT] 437 AGGTCCT Sau3AI 738 GATC ^GATC 840 GATC SfaNI 195 GCATC GCATC(5/9) 485 GCATC StuI 320 AGGCCT AGG^CCT 746 AGGCCT TaqI 723 TCGA T^CGA 1221 TCGA TthHB8I 723 TCGA T^CGA 1221 TCGA XhoII 737 AGATCT [GA]^GATC[CT] 839 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanI BanII BbvI BglI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MwoI NlaIII NlaIV PstI PvuII RsaI Sau96I ScrFI SfcI TseI Tsp45I TspRI