Restriction map for pF1KB4417
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BspDI       BspEI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KasI        KpnI        Ksp632I     MaeI        
MamI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SalI        SapI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SmaI        SnaBI       SpeI        SphI        SspI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Asp700I       259  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          722  CTCGAG               C^[CT]CG[GA]G
AviII         345  TGCGCA               TGC^GCA
BbvCI        1433  CCTCAGC              CCTCAGC(-5/-2)
BsaI          725  GAGACC               GGTCTC(1/5)
BsmBI        1318  GAGACG               CGTCTC(1/5)
BsmI          263  GCATTC               GAATGC(1/-1)
BsoBI         722  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      701  TGTACA               T^GTACA
BspHI         977  TCATGA               T^CATGA
BsrBI         546  GAGCGG               CCGCTC(-3/-3)
BsrFI         340  GCCGGT               [GA]^CCGG[CT]
BsrGI         701  TGTACA               T^GTACA
BstXI         784  CCATCAACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        340  GCCGGT               [GA]^CCGG[CT]
EaeI          906  TGGCCG               [CT]^GGCC[GA]
EcoNI         759  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       588  CCATGG               C^C[AT][AT]GG
FspI          345  TGCGCA               TGC^GCA
HaeII         215  AGCGCC               [GA]GCGC^[CT]
HincII       1240  GTCAAC               GT[CT]^[GA]AC
HindII       1240  GTCAAC               GT[CT]^[GA]AC
KspI         1202  CCGCGG               CCGC^GG
NcoI          588  CCATGG               C^CATGG
NdeI         1229  CATATG               CA^TATG
NspI          572  ACATGC               [GA]CATG^[CT]
PaeR7I        722  CTCGAG               C^TCGAG
PflMI         605  CCAAGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         672  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          977  TCATGA               T^CATGA
SacII        1202  CCGCGG               CCGC^GG
SbfI          528  CCTGCAGG             CCTGCA^GG
SgrAI         339  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI          722  CTCGAG               C^T[CT][GA]AG
Sse8387I      528  CCTGCAGG             CCTGCA^GG
SspBI         701  TGTACA               T^GTACA
StyI          588  CCATGG               C^C[AT][AT]GG
TfiI         1223  GAATC                G^A[AT]TC
Van91I        605  CCAAGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          605  CCAAGTTCTGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          722  CTCGAG               C^TCGAG
XmnI          259  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII        1203  CGCG                 CG^CG
             1270  CGCG                
AcyI          592  GGCGTC               G[GA]^CG[CT]C
             1069  GGCGTC              
AocI          268  CCTGAGG              CC^T[ACGT]AGG
             1392  CCTGAGG             
ApaI          205  GGGCCC               GGGCC^C
              508  GGGCCC              
BcnI          797  CCCGG                CC^[GC]GG
              953  CCCGG               
BglII         737  AGATCT               A^GATCT
              839  AGATCT              
BsaHI         592  GGCGTC               G[GA]^CG[CT]C
             1069  GGCGTC              
BseRI          43  GAGGAG               GAGGAG(10/8)
              433  GAGGAG              
BspMI         527  ACCTGC               ACCTGC(4/8)
              986  ACCTGC              
BstAPI        531  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1451  GCAACGCCTGC         
BstUI        1203  CGCG                 CG^CG
             1270  CGCG                
BstYI         737  AGATCT               [GA]^GATC[CT]
              839  AGATCT              
Bsu36I        268  CCTGAGG              CC^T[ACGT]AGG
             1392  CCTGAGG             
DpnII         738  GATC                 ^GATC
              840  GATC                
Eco81I        268  CCTGAGG              CC^T[ACGT]AGG
             1392  CCTGAGG             
Hin1I         592  GGCGTC               G[GA]^CG[CT]C
             1069  GGCGTC              
MboI          738  GATC                 ^GATC
              840  GATC                
MflI          737  AGATCT               [GA]^GATC[CT]
              839  AGATCT              
MvnI         1203  CGCG                 CG^CG
             1270  CGCG                
NciI          797  CCCGG                CC^[GC]GG
              953  CCCGG               
NdeII         738  GATC                 ^GATC
              840  GATC                
PleI          162  GACTC                GAGTC(4/5)
             1405  GACTC               
PpuMI         113  GGGTCCT              [GA]G^G[AT]CC[CT]
              437  AGGTCCT             
Sau3AI        738  GATC                 ^GATC
              840  GATC                
SfaNI         195  GCATC                GCATC(5/9)
              485  GCATC               
StuI          320  AGGCCT               AGG^CCT
              746  AGGCCT              
TaqI          723  TCGA                 T^CGA
             1221  TCGA                
TthHB8I       723  TCGA                 T^CGA
             1221  TCGA                
XhoII         737  AGATCT               [GA]^GATC[CT]
              839  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BanI        BanII       BbvI        BglI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NlaIII      
NlaIV       PstI        PvuII       RsaI        Sau96I      
ScrFI       SfcI        TseI        Tsp45I      TspRI       
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