Restriction map for
pF1KB4653
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BsgI BsiEI
BsiHKAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MscI
MseI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau96I SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 52 TCCGGA T^CCGGA
BanII 532 GAGCCC G[GA]GC[CT]^C
BciVI 189 GGATAC GTATCC(6/5)
BmyI 532 GAGCCC G[AGT]GC[ACT]^C
BsaWI 52 TCCGGA [AT]^CCGG[AT]
BseAI 52 TCCGGA T^CCGGA
BseRI 566 GAGGAG GAGGAG(10/8)
BsiWI 14 CGTACG C^GTACG
BsmAI 448 GAGAC GTCTC(1/5)
Bsp1286I 532 GAGCCC G[AGT]GC[ACT]^C
BspEI 52 TCCGGA T^CCGGA
BsrDI 244 GCAATG GCAATG(2/0)
BssKI 201 CCAGG ^CC[ACGT]GG
BstAPI 590 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 512 TTCGAA TT^CGAA
BstF5I 90 GGATG GGATG(2/0)
BstNI 201 CCAGG CC^[AT]GG
CfoI 242 GCGC GCG^C
DdeI 310 CTTAG C^T[ACGT]AG
EarI 585 GAAGAG CTCTTC(1/4)
EcoRII 201 CCAGG ^CC[AT]GG
FokI 90 GGATG GGATG(9/13)
HaeIII 199 GGCC GG^CC
HapII 53 CCGG C^CGG
HgaI 71 GCGTC GACGC(5/10)
HhaI 242 GCGC GCG^C
HinP1I 242 GCGC G^CGC
HinfI 49 GATTC G^A[ACGT]TC
HpaII 53 CCGG C^CGG
HphI 272 TCACC GGTGA(8/7)
Ksp632I 585 GAAGAG CTCTTC(1/4)
MroI 52 TCCGGA T^CCGGA
MslI 328 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 53 CCGG C^CGG
MvaI 201 CCAGG CC^[AT]GG
NspI 327 GCATGC [GA]CATG^[CT]
NspV 512 TTCGAA TT^CGAA
PshAI 66 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 585 GAAGAGC GCTCTTC(1/4)
ScrFI 201 CCAGG CC^[ACGT]GG
SfaNI 94 GATGC GCATC(5/9)
SfuI 512 TTCGAA TT^CGAA
SpeI 451 ACTAGT A^CTAGT
SphI 327 GCATGC GCATG^C
TfiI 49 GATTC G^A[AT]TC
Tsp45I 574 GTGAC ^GT[GC]AC
Tsp509I 155 AATT ^AATT
XcmI 332 CCAATGAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 15 GTAC GT^AC
394 GTAC
BbsI 9 GAAGAC GAAGAC(2/6)
63 GAAGAC
BbvI 33 GCTGC GCAGC(8/12)
596 GCTGC
BpuAI 9 GAAGAC GAAGAC(2/6)
63 GAAGAC
BsiYI 39 CCTGATCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
53 CCGGAGTGGGG
BslI 39 CCTGATCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
53 CCGGAGTGGGG
BspMI 20 ACCTGC ACCTGC(4/8)
67 ACCTGC
Cac8I 196 GCAGGC GC[ACGT]^[ACGT]GC
327 GCATGC
EcoRV 505 GATATC GAT^ATC
553 GATATC
Fnu4HI 33 GCTGC GC^[ACGT]GC
596 GCTGC
ItaI 33 GCTGC GC^[ACGT]GC
596 GCTGC
MaeII 13 ACGT A^CGT
493 ACGT
MaeIII 354 GTTAC ^GT[ACGT]AC
574 GTGAC
RsaI 15 GTAC GT^AC
394 GTAC
SfcI 178 CTACAG C^T[GA][CT]AG
234 CTACAG
TaqI 476 TCGA T^CGA
513 TCGA
TseI 33 GCTGC G^C[AT]GC
596 GCTGC
TspRI 540 CAGTG CA[GC]TG[ACGT][ACGT]^
561 CAGTG
TthHB8I 476 TCGA T^CGA
513 TCGA
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BfaI DpnII MaeI MboI
MboII MnlI MwoI NdeII NlaIII
Sau3AI