Restriction map for
pF1KB4659
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BssSI Bst1107I BstBI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AatII 831 GACGTC GACGT^C
AccI 226 GTAGAC GT^[AC][GT]AC
AlwI 1053 GATCC GGATC(4/5)
AviII 467 TGCGCA TGC^GCA
BalI 320 TGGCCA TGG^CCA
BbrPI 1360 CACGTG CAC^GTG
BbvCI 896 CCTCAGC CCTCAGC(-5/-2)
BfaI 701 CTAG C^TAG
BsaAI 1360 CACGTG [CT]AC^GT[GA]
Bsp1407I 1316 TGTACA T^GTACA
BsrDI 342 CATTGC GCAATG(2/0)
BsrFI 550 ACCGGC [GA]^CCGG[CT]
BsrGI 1316 TGTACA T^GTACA
BsrI 415 ACTGG ACTGG(1/-1)
BssHII 1384 GCGCGC G^CGCGC
Cfr10I 550 ACCGGC [GA]^CCGG[CT]
EarI 1324 CTCTTC CTCTTC(1/4)
FspI 467 TGCGCA TGC^GCA
Ksp632I 1324 CTCTTC CTCTTC(1/4)
MaeI 701 CTAG C^TAG
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
PmaCI 1360 CACGTG CAC^GTG
PmlI 1360 CACGTG CAC^GTG
SexAI 380 ACCTGGT A^CC[AT]GGT
SmaI 57 CCCGGG CCC^GGG
SspBI 1316 TGTACA T^GTACA
StuI 536 AGGCCT AGG^CCT
XcmI 1191 CCACTGCGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 57 CCCGGG C^CCGGG
XmaI 57 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 1317 GTAC GT^AC
1407 GTAC
AocI 652 CCTGAGG CC^T[ACGT]AGG
1069 CCTGAGG
BbeI 281 GGCGCC GGCGC^C
332 GGCGCC
BglI 346 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
679 GCCCCCCAGGC
BspMI 44 ACCTGC ACCTGC(4/8)
293 ACCTGC
BsrBI 1397 CCGCTC CCGCTC(-3/-3)
1252 GAGCGG
BstAPI 288 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
795 GCAGCAGCTGC
BstEII 421 GGTGACC G^GT[ACGT]ACC
888 GGTGACC
BstPI 421 GGTGACC G^GT[ACGT]ACC
888 GGTGACC
Bsu36I 652 CCTGAGG CC^T[ACGT]AGG
1069 CCTGAGG
Eco81I 652 CCTGAGG CC^T[ACGT]AGG
1069 CCTGAGG
EcoNI 159 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
648 CCTCCCTGAGG
EcoO65I 421 GGTGACC G^GT[ACGT]ACC
888 GGTGACC
HgaI 65 GCGTC GACGC(5/10)
1196 GCGTC
KasI 281 GGCGCC G^GCGCC
332 GGCGCC
MaeIII 422 GTGAC ^GT[ACGT]AC
889 GTGAC
NarI 281 GGCGCC GG^CGCC
332 GGCGCC
NcoI 508 CCATGG C^CATGG
1038 CCATGG
PaeR7I 122 CTCGAG C^TCGAG
181 CTCGAG
PpuMI 155 AGGTCCT [GA]G^G[AT]CC[CT]
728 GGGACCC
PstI 409 CTGCAG CTGCA^G
802 CTGCAG
RsaI 1317 GTAC GT^AC
1407 GTAC
SacI 31 GAGCTC GAGCT^C
577 GAGCTC
SfoI 281 GGCGCC GGC^GCC
332 GGCGCC
SphI 791 GCATGC GCATG^C
1388 GCATGC
TaqI 123 TCGA T^CGA
182 TCGA
Tsp45I 422 GTGAC ^GT[GC]AC
889 GTGAC
TthHB8I 123 TCGA T^CGA
182 TCGA
XhoI 122 CTCGAG C^TCGAG
181 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TseI TspRI Van91I