Restriction map for
pF1KB4674
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FseI FspI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MfeI MluI MslI MunI
NarI NdeI NheI NotI NruI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 177 TCCGGA T^CCGGA
AcsI 437 AAATTC [GA]^AATT[CT]
AcyI 68 GGCGTC G[GA]^CG[CT]C
AfaI 192 GTAC GT^AC
AlwNI 50 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 437 AAATTC [GA]^AATT[CT]
Asp700I 213 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 185 CCCGGG C^[CT]CG[GA]G
AvaII 548 GGTCC G^G[AT]CC
BalI 254 TGGCCA TGG^CCA
BbvCI 55 GCTGAGG CCTCAGC(-5/-2)
BcgI 63 GCACTGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 416 CTGGAG CTGGAG(16/14)
BsaBI 691 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 68 GGCGTC G[GA]^CG[CT]C
BseAI 177 TCCGGA T^CCGGA
BsmAI 332 GAGAC GTCTC(1/5)
BsoBI 185 CCCGGG C^[CT]CG[GA]G
BspEI 177 TCCGGA T^CCGGA
BspMI 147 GCAGGT ACCTGC(4/8)
BsrFI 22 GCCGGC [GA]^CCGG[CT]
BssSI 985 CACGAG CACGAG(-5/-1)
BstAPI 604 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1157 TTCGAA TT^CGAA
BstXI 1129 CCAGACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
DsaI 866 CCATGG C^C[GA][CT]GG
EaeI 254 TGGCCA [CT]^GGCC[GA]
EcoT22I 681 ATGCAT ATGCA^T
HgaI 69 GCGTC GACGC(5/10)
Hin1I 68 GGCGTC G[GA]^CG[CT]C
HindIII 880 AAGCTT A^AGCTT
HphI 283 GGTGA GGTGA(8/7)
MamI 691 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 254 TGGCCA TGG^CCA
MroI 177 TCCGGA T^CCGGA
MscI 254 TGGCCA TGG^CCA
MspA1I 740 CAGCGG C[AC]G^C[GT]G
NaeI 22 GCCGGC GCC^GGC
NcoI 866 CCATGG C^CATGG
NgoMIV 22 GCCGGC G^CCGGC
NsiI 681 ATGCAT ATGCA^T
NspV 1157 TTCGAA TT^CGAA
PstI 269 CTGCAG CTGCA^G
RsaI 192 GTAC GT^AC
SfuI 1157 TTCGAA TT^CGAA
SmaI 185 CCCGGG CCC^GGG
SspI 276 AATATT AAT^ATT
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
XmnI 213 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanII 181 GAGCCC G[GA]GC[CT]^C
575 GGGCTC
BcnI 185 CCCGG CC^[GC]GG
186 CCGGG
BfaI 820 CTAG C^TAG
1057 CTAG
BsaWI 177 TCCGGA [AT]^CCGG[AT]
545 TCCGGT
BsrDI 429 CATTGC GCAATG(2/0)
616 CATTGC
CfoI 83 GCGC GCG^C
94 GCGC
Cfr13I 135 GGCCC G^G[ACGT]CC
548 GGTCC
HhaI 83 GCGC GCG^C
94 GCGC
HinP1I 83 GCGC G^CGC
94 GCGC
MaeI 820 CTAG C^TAG
1057 CTAG
MboII 286 GAAGA GAAGA(8/7)
745 GAAGA
NciI 185 CCCGG CC^[GC]GG
186 CCGGG
PleI 305 GAGTC GAGTC(4/5)
594 GAGTC
Sau96I 135 GGCCC G^G[ACGT]CC
548 GGTCC
TaqI 125 TCGA T^CGA
1158 TCGA
Tsp45I 412 GTCAC ^GT[GC]AC
983 GTCAC
TthHB8I 125 TCGA T^CGA
1158 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BclI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I DdeI
DpnII EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MboI MflI
MnlI MseI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I TspRI XhoII