Restriction map for
pF1KB4697
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BciVI
BclI BcnI BfaI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I BsrBI BsrDI BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HinfI HpaI KasI
MaeI MamI MfeI MluI MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspI
SwaI TfiI Tth111I Van91I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 710 GGTACC G^GTACC
AccI 472 GTCTAC GT^[AC][GT]AC
AccIII 242 TCCGGA T^CCGGA
AcsI 658 GAATTC [GA]^AATT[CT]
AflII 664 CTTAAG C^TTAAG
AflIII 770 ACGTGT A^C[GA][CT]GT
ApaI 151 GGGCCC GGGCC^C
ApoI 658 GAATTC [GA]^AATT[CT]
Asp718I 710 GGTACC G^GTACC
AspHI 563 GAGCTC G[AT]GC[AT]^C
BalI 71 TGGCCA TGG^CCA
BbvCI 336 CCTCAGC CCTCAGC(-5/-2)
BcgI 425 GCATCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 664 CTTAAG C^TTAAG
BsaI 586 GAGACC GGTCTC(1/5)
BsaWI 242 TCCGGA [AT]^CCGG[AT]
BseAI 242 TCCGGA T^CCGGA
BseRI 298 CTCCTC GAGGAG(10/8)
BsiHKAI 563 GAGCTC G[AT]GC[AT]^C
BsmAI 586 GAGAC GTCTC(1/5)
Bsp1407I 827 TGTACA T^GTACA
BspEI 242 TCCGGA T^CCGGA
BspMI 903 GCAGGT ACCTGC(4/8)
BsrGI 827 TGTACA T^GTACA
BsrI 95 ACTGG ACTGG(1/-1)
BssHII 110 GCGCGC G^CGCGC
BstAPI 24 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 803 CCATCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoRI 658 GAATTC G^AATTC
HaeII 722 GGCGCT [GA]GCGC^[CT]
KpnI 710 GGTACC GGTAC^C
MluNI 71 TGGCCA TGG^CCA
MroI 242 TCCGGA T^CCGGA
MscI 71 TGGCCA TGG^CCA
MslI 804 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 421 GCCGGC GCC^GGC
NgoMIV 421 GCCGGC G^CCGGC
NspI 953 GCATGC [GA]CATG^[CT]
PaeR7I 726 CTCGAG C^TCGAG
PpuMI 926 AGGACCT [GA]G^G[AT]CC[CT]
SacI 563 GAGCTC GAGCT^C
SbfI 900 CCTGCAGG CCTGCA^GG
SphI 953 GCATGC GCATG^C
Sse8387I 900 CCTGCAGG CCTGCA^GG
SspBI 827 TGTACA T^GTACA
Tsp509I 659 AATT ^AATT
XcmI 131 CCAACCTGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 726 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 595 GGCGTC G[GA]^CG[CT]C
669 GGCGTC
AlwNI 25 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
925 CAGGACCTG
AvaI 498 CCCGAG C^[CT]CG[GA]G
726 CTCGAG
BsaHI 595 GGCGTC G[GA]^CG[CT]C
669 GGCGTC
BsiEI 12 CGACCG CG[GA][CT]^CG
41 CGGCCG
BsmFI 512 GGGAC GGGAC(10/14)
767 GGGAC
BsoBI 498 CCCGAG C^[CT]CG[GA]G
726 CTCGAG
BsrFI 421 GCCGGC [GA]^CCGG[CT]
713 ACCGGC
BstEII 493 GGTGACC G^GT[ACGT]ACC
855 GGTGACC
BstPI 493 GGTGACC G^GT[ACGT]ACC
855 GGTGACC
BstYI 221 GGATCT [GA]^GATC[CT]
386 GGATCT
Cfr10I 421 GCCGGC [GA]^CCGG[CT]
713 ACCGGC
DraII 151 GGGCCCC [GA]G^G[ACGT]CC[CT]
926 AGGACCT
EaeI 41 CGGCCG [CT]^GGCC[GA]
71 TGGCCA
EarI 430 CTCTTC CTCTTC(1/4)
678 CTCTTC
EcoO109I 151 GGGCCCC [GA]G^G[ACGT]CC[CT]
926 AGGACCT
EcoO65I 493 GGTGACC G^GT[ACGT]ACC
855 GGTGACC
Hin1I 595 GGCGTC G[GA]^CG[CT]C
669 GGCGTC
Ksp632I 430 CTCTTC CTCTTC(1/4)
678 CTCTTC
KspI 15 CCGCGG CCGC^GG
171 CCGCGG
MaeII 770 ACGT A^CGT
947 ACGT
MflI 221 GGATCT [GA]^GATC[CT]
386 GGATCT
MseI 644 TTAA T^TAA
665 TTAA
MspA1I 15 CCGCGG C[AC]G^C[GT]G
171 CCGCGG
SacII 15 CCGCGG CCGC^GG
171 CCGCGG
StuI 444 AGGCCT AGG^CCT
752 AGGCCT
TaqI 215 TCGA T^CGA
727 TCGA
Tru9I 644 TTAA T^TAA
665 TTAA
TspRI 235 CACTG CA[GC]TG[ACGT][ACGT]^
417 CAGTG
TthHB8I 215 TCGA T^CGA
727 TCGA
XhoII 221 GGATCT [GA]^GATC[CT]
386 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BanII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I