################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## Acc65I AccI AccII AccIII AciI AclI AcsI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 175 GACGTC GACGT^C AcyI 175 GACGTC G[GA]^CG[CT]C AflIII 414 ACGTGT A^C[GA][CT]GT AlwI 1030 GGATC GGATC(4/5) AlwNI 271 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 646 GAGCTC G[AT]GC[AT]^C AvaI 429 CTCGGG C^[CT]CG[GA]G BbsI 218 GTCTTC GAAGAC(2/6) BbvCI 629 CCTCAGC CCTCAGC(-5/-2) BciVI 614 GTATCC GTATCC(6/5) BfaI 200 CTAG C^TAG BpmI 643 CTGGAG CTGGAG(16/14) BpuAI 218 GTCTTC GAAGAC(2/6) BsaAI 413 TACGTG [CT]AC^GT[GA] BsaHI 175 GACGTC G[GA]^CG[CT]C BsaI 819 GAGACC GGTCTC(1/5) BsiHKAI 646 GAGCTC G[AT]GC[AT]^C BsmI 831 GAATGC GAATGC(1/-1) BsoBI 429 CTCGGG C^[CT]CG[GA]G BspMI 822 ACCTGC ACCTGC(4/8) BstXI 635 CCAAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1030 GGATCT [GA]^GATC[CT] DpnII 1031 GATC ^GATC DrdI 436 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 719 CTCTTC CTCTTC(1/4) EcoT14I 674 CCATGG C^C[AT][AT]GG EcoT22I 122 ATGCAT ATGCA^T Hin1I 175 GACGTC G[GA]^CG[CT]C HindIII 138 AAGCTT A^AGCTT Ksp632I 719 CTCTTC CTCTTC(1/4) MaeI 200 CTAG C^TAG MboI 1031 GATC ^GATC MfeI 209 CAATTG C^AATTG MflI 1030 GGATCT [GA]^GATC[CT] MunI 209 CAATTG C^AATTG NcoI 674 CCATGG C^CATGG NdeI 1067 CATATG CA^TATG NdeII 1031 GATC ^GATC NsiI 122 ATGCAT ATGCA^T PleI 988 GAGTC GAGTC(4/5) PstI 1034 CTGCAG CTGCA^G SacI 646 GAGCTC GAGCT^C Sau3AI 1031 GATC ^GATC SfcI 1034 CTGCAG C^T[GA][CT]AG StuI 593 AGGCCT AGG^CCT StyI 674 CCATGG C^C[AT][AT]GG TspRI 95 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 1030 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanII 358 GGGCTC G[GA]GC[CT]^C 646 GAGCTC BmyI 358 GGGCTC G[AGT]GC[ACT]^C 646 GAGCTC BseRI 757 GAGGAG GAGGAG(10/8) 890 GAGGAG BsiYI 861 CCCAGAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 862 CCAGAGATGGG BslI 861 CCCAGAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 862 CCAGAGATGGG BsmFI 870 GGGAC GGGAC(10/14) 715 GTCCC Bsp1286I 358 GGGCTC G[AGT]GC[ACT]^C 646 GAGCTC Cac8I 48 GCCAGC GC[ACGT]^[ACGT]GC 648 GCTCGC DsaI 354 CCGTGG C^C[GA][CT]GG 674 CCATGG MspA1I 50 CAGCTG C[AC]G^C[GT]G 1081 CAGCTG PvuII 50 CAGCTG CAG^CTG 1081 CAGCTG SfaNI 124 GCATC GCATC(5/9) 845 GCATC TaqI 80 TCGA T^CGA 744 TCGA TfiI 895 GATTC G^A[AT]TC 1039 GAATC TthHB8I 80 TCGA T^CGA 744 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI BbvI BsaJI BsmAI BsrI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboII MnlI MseI MslI MvaI MwoI NlaIII ScrFI SmlI Tru9I TseI Tsp45I Tsp509I