Restriction map for pF1KB4707
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AciI        
AclI        AcsI        AfaI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MluI        MluNI       MroI        MscI        MspI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NlaIV       NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         175  GACGTC               GACGT^C
AcyI          175  GACGTC               G[GA]^CG[CT]C
AflIII        414  ACGTGT               A^C[GA][CT]GT
AlwI         1030  GGATC                GGATC(4/5)
AlwNI         271  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         646  GAGCTC               G[AT]GC[AT]^C
AvaI          429  CTCGGG               C^[CT]CG[GA]G
BbsI          218  GTCTTC               GAAGAC(2/6)
BbvCI         629  CCTCAGC              CCTCAGC(-5/-2)
BciVI         614  GTATCC               GTATCC(6/5)
BfaI          200  CTAG                 C^TAG
BpmI          643  CTGGAG               CTGGAG(16/14)
BpuAI         218  GTCTTC               GAAGAC(2/6)
BsaAI         413  TACGTG               [CT]AC^GT[GA]
BsaHI         175  GACGTC               G[GA]^CG[CT]C
BsaI          819  GAGACC               GGTCTC(1/5)
BsiHKAI       646  GAGCTC               G[AT]GC[AT]^C
BsmI          831  GAATGC               GAATGC(1/-1)
BsoBI         429  CTCGGG               C^[CT]CG[GA]G
BspMI         822  ACCTGC               ACCTGC(4/8)
BstXI         635  CCAAAACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1030  GGATCT               [GA]^GATC[CT]
DpnII        1031  GATC                 ^GATC
DrdI          436  GACATGAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          719  CTCTTC               CTCTTC(1/4)
EcoT14I       674  CCATGG               C^C[AT][AT]GG
EcoT22I       122  ATGCAT               ATGCA^T
Hin1I         175  GACGTC               G[GA]^CG[CT]C
HindIII       138  AAGCTT               A^AGCTT
Ksp632I       719  CTCTTC               CTCTTC(1/4)
MaeI          200  CTAG                 C^TAG
MboI         1031  GATC                 ^GATC
MfeI          209  CAATTG               C^AATTG
MflI         1030  GGATCT               [GA]^GATC[CT]
MunI          209  CAATTG               C^AATTG
NcoI          674  CCATGG               C^CATGG
NdeI         1067  CATATG               CA^TATG
NdeII        1031  GATC                 ^GATC
NsiI          122  ATGCAT               ATGCA^T
PleI          988  GAGTC                GAGTC(4/5)
PstI         1034  CTGCAG               CTGCA^G
SacI          646  GAGCTC               GAGCT^C
Sau3AI       1031  GATC                 ^GATC
SfcI         1034  CTGCAG               C^T[GA][CT]AG
StuI          593  AGGCCT               AGG^CCT
StyI          674  CCATGG               C^C[AT][AT]GG
TspRI          95  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII        1030  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BanII         358  GGGCTC               G[GA]GC[CT]^C
              646  GAGCTC              
BmyI          358  GGGCTC               G[AGT]GC[ACT]^C
              646  GAGCTC              
BseRI         757  GAGGAG               GAGGAG(10/8)
              890  GAGGAG              
BsiYI         861  CCCAGAGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              862  CCAGAGATGGG         
BslI          861  CCCAGAGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              862  CCAGAGATGGG         
BsmFI         870  GGGAC                GGGAC(10/14)
              715  GTCCC               
Bsp1286I      358  GGGCTC               G[AGT]GC[ACT]^C
              646  GAGCTC              
Cac8I          48  GCCAGC               GC[ACGT]^[ACGT]GC
              648  GCTCGC              
DsaI          354  CCGTGG               C^C[GA][CT]GG
              674  CCATGG              
MspA1I         50  CAGCTG               C[AC]G^C[GT]G
             1081  CAGCTG              
PvuII          50  CAGCTG               CAG^CTG
             1081  CAGCTG              
SfaNI         124  GCATC                GCATC(5/9)
              845  GCATC               
TaqI           80  TCGA                 T^CGA
              744  TCGA                
TfiI          895  GATTC                G^A[AT]TC
             1039  GAATC               
TthHB8I        80  TCGA                 T^CGA
              744  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsmAI       BsrI        
BssKI       BstF5I      BstNI       DdeI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeII       MaeIII      MboII       MnlI        
MseI        MslI        MvaI        MwoI        NlaIII      
ScrFI       SmlI        Tru9I       TseI        Tsp45I      
Tsp509I     
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