Restriction map for
pF1KB4708
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbeI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 559 GTCTAC GT^[AC][GT]AC
AcyI 1315 GACGCC G[GA]^CG[CT]C
AocI 345 CCTGAGG CC^T[ACGT]AGG
Asp700I 369 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1150 GAGCAC G[AT]GC[AT]^C
AvaI 159 CCCGGG C^[CT]CG[GA]G
AviII 92 TGCGCA TGC^GCA
BamHI 687 GGATCC G^GATCC
BbsI 351 GAAGAC GAAGAC(2/6)
BbvCI 358 GCTGAGG CCTCAGC(-5/-2)
BpuAI 351 GAAGAC GAAGAC(2/6)
BsaHI 1315 GACGCC G[GA]^CG[CT]C
BsaWI 911 TCCGGT [AT]^CCGG[AT]
BsgI 706 CTGCAC GTGCAG(16/14)
BsiHKAI 1150 GAGCAC G[AT]GC[AT]^C
BsmAI 1212 GTCTC GTCTC(1/5)
BsmFI 1284 GTCCC GGGAC(10/14)
BsoBI 159 CCCGGG C^[CT]CG[GA]G
BspHI 851 TCATGA T^CATGA
BstYI 687 GGATCC [GA]^GATC[CT]
Bsu36I 345 CCTGAGG CC^T[ACGT]AGG
DsaI 306 CCACGG C^C[GA][CT]GG
EarI 1159 CTCTTC CTCTTC(1/4)
Eco81I 345 CCTGAGG CC^T[ACGT]AGG
EcoRI 238 GAATTC G^AATTC
FspI 92 TGCGCA TGC^GCA
HgaI 1315 GACGC GACGC(5/10)
Hin1I 1315 GACGCC G[GA]^CG[CT]C
Ksp632I 1159 CTCTTC CTCTTC(1/4)
MflI 687 GGATCC [GA]^GATC[CT]
NspI 701 ACATGC [GA]CATG^[CT]
PflMI 629 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1282 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 851 TCATGA T^CATGA
SfcI 561 CTACAG C^T[GA][CT]AG
SmaI 159 CCCGGG CCC^GGG
StuI 1049 AGGCCT AGG^CCT
TaqI 740 TCGA T^CGA
TthHB8I 740 TCGA T^CGA
Van91I 629 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1085 CCACCTGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 687 GGATCC [GA]^GATC[CT]
XmaCI 159 CCCGGG C^CCGGG
XmaI 159 CCCGGG C^CCGGG
XmnI 369 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 40 CGCG CG^CG
217 CGCG
AcsI 238 GAATTC [GA]^AATT[CT]
595 AAATTC
AfaI 744 GTAC GT^AC
840 GTAC
AflIII 389 ACGTGT A^C[GA][CT]GT
710 ACGTGT
AlwI 688 GATCC GGATC(4/5)
687 GGATC
ApaI 341 GGGCCC GGGCC^C
1262 GGGCCC
ApoI 238 GAATTC [GA]^AATT[CT]
595 AAATTC
BalI 546 TGGCCA TGG^CCA
606 TGGCCA
BanI 51 GGTGCC G^G[CT][GA]CC
619 GGTGCC
BbrPI 388 CACGTG CAC^GTG
709 CACGTG
BsaAI 388 CACGTG [CT]AC^GT[GA]
709 CACGTG
BsrDI 435 CATTGC GCAATG(2/0)
464 GCAATG
BstAPI 84 GCATAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
202 GCAACGGATGC
BstUI 40 CGCG CG^CG
217 CGCG
Cac8I 167 GCCAGC GC[ACGT]^[ACGT]GC
1073 GCCAGC
EaeI 546 TGGCCA [CT]^GGCC[GA]
606 TGGCCA
FauI 1200 CCCGC CCCGC(4/6)
171 GCGGG
MaeIII 1009 GTCAC ^GT[ACGT]AC
1058 GTGAC
MluNI 546 TGGCCA TGG^CCA
606 TGGCCA
MscI 546 TGGCCA TGG^CCA
606 TGGCCA
MseI 613 TTAA T^TAA
1136 TTAA
MvnI 40 CGCG CG^CG
217 CGCG
PmaCI 388 CACGTG CAC^GTG
709 CACGTG
PmlI 388 CACGTG CAC^GTG
709 CACGTG
PvuII 63 CAGCTG CAG^CTG
1075 CAGCTG
RsaI 744 GTAC GT^AC
840 GTAC
Tru9I 613 TTAA T^TAA
1136 TTAA
Tsp45I 1009 GTCAC ^GT[GC]AC
1058 GTGAC
Tsp509I 239 AATT ^AATT
596 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TfiI TseI TspRI