Restriction map for
pF1KB4835
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbsI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 526 GGTACC G^GTACC
AcsI 403 GAATTC [GA]^AATT[CT]
AfaI 527 GTAC GT^AC
AflIII 725 ACATGT A^C[GA][CT]GT
AlwNI 1222 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 160 GGGCCC GGGCC^C
ApoI 403 GAATTC [GA]^AATT[CT]
Asp718I 526 GGTACC G^GTACC
AspHI 654 GAGCTC G[AT]GC[AT]^C
BbvCI 63 GCTGAGG CCTCAGC(-5/-2)
BfaI 1021 CTAG C^TAG
BglI 162 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1017 AGATCT A^GATCT
BsiHKAI 654 GAGCTC G[AT]GC[AT]^C
BsmFI 748 GGGAC GGGAC(10/14)
BsmI 591 GAATGC GAATGC(1/-1)
BspLU11I 725 ACATGT A^CATGT
BsrDI 971 GCAATG GCAATG(2/0)
BsrFI 523 GCCGGT [GA]^CCGG[CT]
BsrI 907 CCAGT ACTGG(1/-1)
BssSI 208 CACGAG CACGAG(-5/-1)
BstXI 273 CCAGATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1017 AGATCT [GA]^GATC[CT]
Cfr10I 523 GCCGGT [GA]^CCGG[CT]
DsaI 449 CCACGG C^C[GA][CT]GG
EagI 10 CGGCCG C^GGCCG
EclXI 10 CGGCCG C^GGCCG
Eco52I 10 CGGCCG C^GGCCG
EcoRI 403 GAATTC G^AATTC
EcoT22I 277 ATGCAT ATGCA^T
KpnI 526 GGTACC GGTAC^C
MaeI 1021 CTAG C^TAG
MflI 1017 AGATCT [GA]^GATC[CT]
NsiI 277 ATGCAT ATGCA^T
PpuMI 934 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 527 GTAC GT^AC
SacI 654 GAGCTC GAGCT^C
SexAI 368 ACCTGGT A^CC[AT]GGT
SmaI 1196 CCCGGG CCC^GGG
Tsp509I 404 AATT ^AATT
XhoII 1017 AGATCT [GA]^GATC[CT]
XmaCI 1196 CCCGGG C^CCGGG
XmaI 1196 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 242 TCCGGA T^CCGGA
1406 TCCGGA
AvaII 935 GGACC G^G[AT]CC
1137 GGACC
BalI 446 TGGCCA TGG^CCA
980 TGGCCA
BbrPI 375 CACGTG CAC^GTG
1189 CACGTG
BcgI 210 CGAGTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
585 CGACCAGAATGC
BsaAI 375 CACGTG [CT]AC^GT[GA]
1189 CACGTG
BsaWI 242 TCCGGA [AT]^CCGG[AT]
1406 TCCGGA
BseAI 242 TCCGGA T^CCGGA
1406 TCCGGA
BsiEI 10 CGGCCG CG[GA][CT]^CG
140 CGGTCG
BspEI 242 TCCGGA T^CCGGA
1406 TCCGGA
BstAPI 1230 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1323 GCAGGAGGTGC
DpnII 15 GATC ^GATC
1018 GATC
DraII 160 GGGCCCC [GA]G^G[ACGT]CC[CT]
934 AGGACCT
EcoO109I 160 GGGCCCC [GA]G^G[ACGT]CC[CT]
934 AGGACCT
EcoT14I 306 CCAAGG C^C[AT][AT]GG
767 CCTTGG
MboI 15 GATC ^GATC
1018 GATC
MboII 1036 GAAGA GAAGA(8/7)
473 TCTTC
MluNI 446 TGGCCA TGG^CCA
980 TGGCCA
MroI 242 TCCGGA T^CCGGA
1406 TCCGGA
MscI 446 TGGCCA TGG^CCA
980 TGGCCA
NdeII 15 GATC ^GATC
1018 GATC
NspI 725 ACATGT [GA]CATG^[CT]
995 ACATGC
PflMI 470 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
993 CCACATGCTGG
PleI 888 GACTC GAGTC(4/5)
284 GAGTC
PmaCI 375 CACGTG CAC^GTG
1189 CACGTG
PmlI 375 CACGTG CAC^GTG
1189 CACGTG
Sau3AI 15 GATC ^GATC
1018 GATC
StuI 782 AGGCCT AGG^CCT
1023 AGGCCT
StyI 306 CCAAGG C^C[AT][AT]GG
767 CCTTGG
TaqI 143 TCGA T^CGA
347 TCGA
TthHB8I 143 TCGA T^CGA
347 TCGA
Van91I 470 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
993 CCACATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AvaI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PvuII Sau96I
ScrFI SfaNI TfiI TseI Tsp45I
TspRI