Restriction map for
pF1KB4983
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MaeII MamI
MluI MroI MspA1I MspI MvnI
NaeI NarI NciI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AciI 506 GCGG CCGC(-3/-1)
Alw44I 1144 GTGCAC G^TGCAC
AlwNI 1373 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1144 GTGCAC G^TGCAC
BalI 32 TGGCCA TGG^CCA
BstAPI 1489 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1083 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 224 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1007 GAATTC G^AATTC
EcoRV 768 GATATC GAT^ATC
HgaI 1501 GCGTC GACGC(5/10)
MfeI 1158 CAATTG C^AATTG
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
MunI 1158 CAATTG C^AATTG
NcoI 303 CCATGG C^CATGG
NdeI 295 CATATG CA^TATG
NspI 235 ACATGC [GA]CATG^[CT]
PflMI 1372 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1371 ACCAGGT A^CC[AT]GGT
SwaI 813 ATTTAAAT ATTT^AAAT
TfiI 575 GAATC G^A[AT]TC
Van91I 1372 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 322 TCTAGA T^CTAGA
XcmI 294 CCATATGGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 994 GATCC GGATC(4/5)
1320 GATCC
AspHI 1144 GTGCAC G[AT]GC[AT]^C
1355 GAGCAC
BmyI 1144 GTGCAC G[AGT]GC[ACT]^C
1355 GAGCAC
BsgI 659 GTGCAG GTGCAG(16/14)
707 GTGCAG
BsiHKAI 1144 GTGCAC G[AT]GC[AT]^C
1355 GAGCAC
BsiYI 565 CCTTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1372 CCAGGTGCTGG
BslI 565 CCTTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1372 CCAGGTGCTGG
BsmFI 75 GGGAC GGGAC(10/14)
966 GGGAC
Bsp1286I 1144 GTGCAC G[AGT]GC[ACT]^C
1355 GAGCAC
BstYI 993 AGATCC [GA]^GATC[CT]
1319 AGATCC
DsaI 303 CCATGG C^C[GA][CT]GG
545 CCACGG
EaeI 32 TGGCCA [CT]^GGCC[GA]
939 TGGCCG
HindIII 395 AAGCTT A^AGCTT
1475 AAGCTT
HphI 1060 GGTGA GGTGA(8/7)
1314 GGTGA
MflI 993 AGATCC [GA]^GATC[CT]
1319 AGATCC
MslI 445 CATGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
739 CATGTAGGTG
PleI 12 GAGTC GAGTC(4/5)
1445 GAGTC
PstI 83 CTGCAG CTGCA^G
386 CTGCAG
SapI 973 GAAGAGC GCTCTTC(1/4)
1216 GAAGAGC
ScaI 382 AGTACT AGT^ACT
1347 AGTACT
TaqI 54 TCGA T^CGA
1336 TCGA
Tsp45I 484 GTCAC ^GT[GC]AC
715 GTGAC
TthHB8I 54 TCGA T^CGA
1336 TCGA
XhoII 993 AGATCC [GA]^GATC[CT]
1319 AGATCC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BfaI BpmI BsaJI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp509I TspRI