Restriction map for
pF1KB5000
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HindIII KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 143 CGCG CG^CG
AcyI 320 GACGCC G[GA]^CG[CT]C
AocI 226 CCTAAGG CC^T[ACGT]AGG
AvaII 72 GGTCC G^G[AT]CC
BpmI 592 CTCCAG CTGGAG(16/14)
BsaHI 320 GACGCC G[GA]^CG[CT]C
BseRI 307 GAGGAG GAGGAG(10/8)
BssHII 142 GCGCGC G^CGCGC
BstF5I 18 CATCC GGATG(2/0)
BstNI 21 CCAGG CC^[AT]GG
BstUI 143 CGCG CG^CG
BstXI 543 CCACACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 226 CCTAAGG CC^T[ACGT]AGG
DraIII 441 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 207 TGGCCG [CT]^GGCC[GA]
Eco81I 226 CCTAAGG CC^T[ACGT]AGG
EcoNI 519 CCTTGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 348 GAATTC G^AATTC
EcoRII 21 CCAGG ^CC[AT]GG
EcoT14I 519 CCTTGG C^C[AT][AT]GG
FauI 119 CCCGC CCCGC(4/6)
FokI 18 CATCC GGATG(9/13)
HgaI 320 GACGC GACGC(5/10)
Hin1I 320 GACGCC G[GA]^CG[CT]C
HincII 265 GTTAAC GT[CT]^[GA]AC
HindII 265 GTTAAC GT[CT]^[GA]AC
HpaI 265 GTTAAC GTT^AAC
MaeII 176 ACGT A^CGT
MseI 266 TTAA T^TAA
MslI 256 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 75 CCGCTG C[AC]G^C[GT]G
MvaI 21 CCAGG CC^[AT]GG
MvnI 143 CGCG CG^CG
PleI 341 GAGTC GAGTC(4/5)
PstI 497 CTGCAG CTGCA^G
SfaNI 480 GATGC GCATC(5/9)
SfcI 497 CTGCAG C^T[GA][CT]AG
StyI 519 CCTTGG C^C[AT][AT]GG
TaqI 285 TCGA T^CGA
Tru9I 266 TTAA T^TAA
TthHB8I 285 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 348 GAATTC [GA]^AATT[CT]
382 AAATTT
AfaI 15 GTAC GT^AC
87 GTAC
AlwI 406 GATCC GGATC(4/5)
449 GGATC
ApoI 348 GAATTC [GA]^AATT[CT]
382 AAATTT
BanII 113 GGGCTC G[GA]GC[CT]^C
332 GGGCTC
BbvI 568 GCAGC GCAGC(8/12)
77 GCTGC
BcnI 128 CCCGG CC^[GC]GG
564 CCGGG
BglI 146 GCCGACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
200 GCCGTGGTGGC
BmyI 113 GGGCTC G[AGT]GC[ACT]^C
332 GGGCTC
BsiYI 324 CCACTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
519 CCTTGGAAAGG
BslI 324 CCACTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
519 CCTTGGAAAGG
Bsp1286I 113 GGGCTC G[AGT]GC[ACT]^C
332 GGGCTC
Cfr13I 72 GGTCC G^G[ACGT]CC
528 GGGCC
DpnII 406 GATC ^GATC
450 GATC
DsaI 201 CCGTGG C^C[GA][CT]GG
513 CCGTGG
Fnu4HI 77 GCTGC GC^[ACGT]GC
568 GCAGC
HapII 129 CCGG C^CGG
564 CCGG
HpaII 129 CCGG C^CGG
564 CCGG
HphI 364 GGTGA GGTGA(8/7)
452 TCACC
ItaI 77 GCTGC GC^[ACGT]GC
568 GCAGC
MaeIII 173 GTTAC ^GT[ACGT]AC
605 GTTAC
MboI 406 GATC ^GATC
450 GATC
MspI 129 CCGG C^CGG
564 CCGG
NciI 128 CCCGG CC^[GC]GG
564 CCGGG
NdeII 406 GATC ^GATC
450 GATC
NlaIII 54 CATG CATG^
259 CATG
NlaIV 71 GGGTCC GG[ACGT]^[ACGT]CC
114 GGCTCC
RsaI 15 GTAC GT^AC
87 GTAC
Sau3AI 406 GATC ^GATC
450 GATC
Sau96I 72 GGTCC G^G[ACGT]CC
528 GGGCC
TfiI 189 GATTC G^A[AT]TC
370 GATTC
TseI 77 GCTGC G^C[AT]GC
568 GCAGC
Tsp509I 349 AATT ^AATT
383 AATT
TspRI 325 CACTG CA[GC]TG[ACGT][ACGT]^
445 CAGTG
XcmI 540 CCACCACACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
543 CCACACTATTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI BsaJI BsrI BssKI
Cac8I CfoI DdeI HaeIII HhaI
HinP1I HinfI MboII MnlI MwoI
ScrFI