Restriction map for
pF1KB5005
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BpuAI BsaBI
BsaI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MseI MunI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 637 AAATTC [GA]^AATT[CT]
Alw44I 319 GTGCAC G^TGCAC
AlwNI 83 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1225 CCTAAGG CC^T[ACGT]AGG
ApaLI 319 GTGCAC G^TGCAC
ApoI 637 AAATTC [GA]^AATT[CT]
AspI 678 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 820 CACGTG CAC^GTG
BfaI 1171 CTAG C^TAG
BglI 142 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 872 AGATCT A^GATCT
BlpI 288 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 288 GCTGAGC GC^T[ACGT]AGC
BsaAI 820 CACGTG [CT]AC^GT[GA]
BsaWI 713 ACCGGA [AT]^CCGG[AT]
BsmAI 1244 GAGAC GTCTC(1/5)
BsmBI 1244 GAGACG CGTCTC(1/5)
BsmFI 1178 GGGAC GGGAC(10/14)
BspHI 701 TCATGA T^CATGA
BsrI 626 ACTGG ACTGG(1/-1)
BssSI 238 CTCGTG CACGAG(-5/-1)
BstYI 872 AGATCT [GA]^GATC[CT]
Bsu36I 1225 CCTAAGG CC^T[ACGT]AGG
CelII 288 GCTGAGC GC^T[ACGT]AGC
DraII 1031 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 261 GACCACGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1225 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1031 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 1176 GCGGG CCCGC(4/6)
HgaI 268 GCGTC GACGC(5/10)
HincII 601 GTCAAC GT[CT]^[GA]AC
HindII 601 GTCAAC GT[CT]^[GA]AC
HinfI 678 GACTC G^A[ACGT]TC
MaeI 1171 CTAG C^TAG
MflI 872 AGATCT [GA]^GATC[CT]
NcoI 170 CCATGG C^CATGG
NspI 1016 GCATGT [GA]CATG^[CT]
PaeR7I 832 CTCGAG C^TCGAG
PflFI 678 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 678 GACTC GAGTC(4/5)
PmaCI 820 CACGTG CAC^GTG
PmlI 820 CACGTG CAC^GTG
PpuMI 1031 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 701 TCATGA T^CATGA
SmlI 832 CTCGAG C^T[CT][GA]AG
StuI 720 AGGCCT AGG^CCT
TaqI 833 TCGA T^CGA
Tsp509I 638 AATT ^AATT
Tth111I 678 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 833 TCGA T^CGA
XcmI 920 CCAAGGGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 832 CTCGAG C^TCGAG
XhoII 872 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AspHI 319 GTGCAC G[AT]GC[AT]^C
469 GAGCAC
AvaI 556 CCCGAG C^[CT]CG[GA]G
832 CTCGAG
AviII 440 TGCGCA TGC^GCA
1127 TGCGCA
BalI 173 TGGCCA TGG^CCA
1043 TGGCCA
BanI 589 GGCGCC G^G[CT][GA]CC
1211 GGCGCC
BanII 108 GAGCCC G[GA]GC[CT]^C
235 GGGCTC
BbeI 589 GGCGCC GGCGC^C
1211 GGCGCC
BpmI 792 CTCCAG CTGGAG(16/14)
37 CTGGAG
BseRI 313 GAGGAG GAGGAG(10/8)
1120 GAGGAG
BsiHKAI 319 GTGCAC G[AT]GC[AT]^C
469 GAGCAC
BsoBI 556 CCCGAG C^[CT]CG[GA]G
832 CTCGAG
BspMI 69 ACCTGC ACCTGC(4/8)
947 ACCTGC
BsrFI 142 GCCGGC [GA]^CCGG[CT]
325 GCCGGC
BssHII 367 GCGCGC G^CGCGC
503 GCGCGC
BstEII 561 GGTCACC G^GT[ACGT]ACC
936 GGTGACC
BstPI 561 GGTCACC G^GT[ACGT]ACC
936 GGTGACC
BstXI 566 CCAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
920 CCAAGGGTGTGG
Cfr10I 142 GCCGGC [GA]^CCGG[CT]
325 GCCGGC
DpnII 480 GATC ^GATC
873 GATC
EcoNI 33 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
975 CCTGACTGAGG
EcoO65I 561 GGTCACC G^GT[ACGT]ACC
936 GGTGACC
FspI 440 TGCGCA TGC^GCA
1127 TGCGCA
KasI 589 GGCGCC G^GCGCC
1211 GGCGCC
MboI 480 GATC ^GATC
873 GATC
MluNI 173 TGGCCA TGG^CCA
1043 TGGCCA
MscI 173 TGGCCA TGG^CCA
1043 TGGCCA
MspA1I 301 CAGCTG C[AC]G^C[GT]G
865 CAGCTG
NaeI 142 GCCGGC GCC^GGC
325 GCCGGC
NarI 589 GGCGCC GG^CGCC
1211 GGCGCC
NdeII 480 GATC ^GATC
873 GATC
NgoMIV 142 GCCGGC G^CCGGC
325 GCCGGC
PvuII 301 CAGCTG CAG^CTG
865 CAGCTG
Sau3AI 480 GATC ^GATC
873 GATC
SfoI 589 GGCGCC GGC^GCC
1211 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MslI MspI MvaI MvnI MwoI
NlaIII NlaIV PflMI PstI RsaI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI Van91I