Restriction map for
pF1KE3523
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tsp509I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
Acc65I 638 GGTACC G^GTACC
AccII 970 CGCG CG^CG
AflIII 501 ACACGT A^C[GA][CT]GT
AlwI 96 GGATC GGATC(4/5)
Asp718I 638 GGTACC G^GTACC
AspI 967 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 584 CCCGGG C^[CT]CG[GA]G
AvaII 516 GGACC G^G[AT]CC
BanI 638 GGTACC G^G[CT][GA]CC
BanII 556 GAGCTC G[GA]GC[CT]^C
BbsI 928 GAAGAC GAAGAC(2/6)
BciVI 813 GGATAC GTATCC(6/5)
BfaI 229 CTAG C^TAG
BpmI 775 CTGGAG CTGGAG(16/14)
BpuAI 928 GAAGAC GAAGAC(2/6)
BsaI 965 GAGACC GGTCTC(1/5)
BsgI 701 GTGCAG GTGCAG(16/14)
BsmBI 972 CGTCTC CGTCTC(1/5)
BsmI 255 GAATGC GAATGC(1/-1)
BsoBI 584 CCCGGG C^[CT]CG[GA]G
BspHI 996 TCATGA T^CATGA
BsrFI 641 ACCGGC [GA]^CCGG[CT]
BstUI 970 CGCG CG^CG
BstYI 96 GGATCT [GA]^GATC[CT]
Cac8I 703 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1010 GCGC GCG^C
Cfr10I 641 ACCGGC [GA]^CCGG[CT]
DpnII 97 GATC ^GATC
DraI 237 TTTAAA TTT^AAA
DsaI 64 CCACGG C^C[GA][CT]GG
EaeI 820 TGGCCG [CT]^GGCC[GA]
FauI 474 GCGGG CCCGC(4/6)
HaeII 1009 AGCGCC [GA]GCGC^[CT]
HgaI 971 GCGTC GACGC(5/10)
HhaI 1010 GCGC GCG^C
HinP1I 1010 GCGC G^CGC
HincII 734 GTCAAC GT[CT]^[GA]AC
HindII 734 GTCAAC GT[CT]^[GA]AC
HindIII 293 AAGCTT A^AGCTT
KpnI 638 GGTACC GGTAC^C
MaeI 229 CTAG C^TAG
MaeII 503 ACGT A^CGT
MboI 97 GATC ^GATC
MflI 96 GGATCT [GA]^GATC[CT]
MseI 238 TTAA T^TAA
MslI 246 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 970 CGCG CG^CG
NdeI 693 CATATG CA^TATG
NdeII 97 GATC ^GATC
PflFI 967 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 515 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 996 TCATGA T^CATGA
SacI 556 GAGCTC GAGCT^C
SapI 123 GAAGAGC GCTCTTC(1/4)
Sau3AI 97 GATC ^GATC
SfcI 806 CTACAG C^T[GA][CT]AG
SmaI 584 CCCGGG CCC^GGG
SpeI 228 ACTAGT A^CTAGT
TaqI 879 TCGA T^CGA
Tru9I 238 TTAA T^TAA
Tth111I 967 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 879 TCGA T^CGA
XhoII 96 GGATCT [GA]^GATC[CT]
XmaCI 584 CCCGGG C^CCGGG
XmaI 584 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 331 GTGCTC G[AT]GC[AT]^C
556 GAGCTC
BsiHKAI 331 GTGCTC G[AT]GC[AT]^C
556 GAGCTC
BsiYI 132 CCCTTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
804 CCCTACAGAGG
BslI 132 CCCTTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
804 CCCTACAGAGG
BsrI 564 ACTGG ACTGG(1/-1)
405 CCAGT
DraII 476 GGGGCCT [GA]G^G[ACGT]CC[CT]
515 GGGACCT
EcoO109I 476 GGGGCCT [GA]G^G[ACGT]CC[CT]
515 GGGACCT
MspA1I 772 CAGCTG C[AC]G^C[GT]G
1024 CAGCTG
NspI 318 ACATGC [GA]CATG^[CT]
386 ACATGC
PvuII 772 CAGCTG CAG^CTG
1024 CAGCTG
SspI 325 AATATT AAT^ATT
674 AATATT
Tsp45I 545 GTCAC ^GT[GC]AC
628 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BmyI BsaJI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
TseI TspRI