Restriction map for
pF1KB5141
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BbvCI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NarI NdeI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 1286 GAATTC [GA]^AATT[CT]
AocI 781 CCTAAGG CC^T[ACGT]AGG
ApaI 970 GGGCCC GGGCC^C
ApoI 1286 GAATTC [GA]^AATT[CT]
Asp700I 1533 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1156 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1238 TGGCCA TGG^CCA
BamHI 410 GGATCC G^GATCC
BbsI 1261 GAAGAC GAAGAC(2/6)
BcgI 1424 GCATCTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1067 TGATCA T^GATCA
BglI 82 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1261 GAAGAC GAAGAC(2/6)
BsiEI 80 CGGCCG CG[GA][CT]^CG
BsmBI 594 CGTCTC CGTCTC(1/5)
BspLU11I 1388 ACATGT A^CATGT
BstAPI 833 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 781 CCTAAGG CC^T[ACGT]AGG
EagI 80 CGGCCG C^GGCCG
EarI 871 GAAGAG CTCTTC(1/4)
EclXI 80 CGGCCG C^GGCCG
Eco52I 80 CGGCCG C^GGCCG
Eco81I 781 CCTAAGG CC^T[ACGT]AGG
EcoRI 1286 GAATTC G^AATTC
FbaI 1067 TGATCA T^GATCA
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
Ksp632I 871 GAAGAG CTCTTC(1/4)
MluNI 1238 TGGCCA TGG^CCA
MscI 1238 TGGCCA TGG^CCA
MseI 1271 TTAA T^TAA
NcoI 1151 CCATGG C^CATGG
NheI 454 GCTAGC G^CTAGC
NotI 79 GCGGCCGC GC^GGCCGC
NspI 1388 ACATGT [GA]CATG^[CT]
PaeR7I 229 CTCGAG C^TCGAG
PflFI 1156 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 414 CCTGCAGG CCTGCA^GG
ScaI 977 AGTACT AGT^ACT
SfiI 81 GGCCGCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 414 CCTGCAGG CCTGCA^GG
StuI 89 AGGCCT AGG^CCT
TfiI 935 GATTC G^A[AT]TC
Tru9I 1271 TTAA T^TAA
Tth111I 1156 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1019 CCAAGGGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 229 CTCGAG C^TCGAG
XmnI 1533 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 289 GACGCC G[GA]^CG[CT]C
679 GACGCC
AflIII 914 ACGTGT A^C[GA][CT]GT
1388 ACATGT
AlwNI 51 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1133 CAGTGTCTG
BfaI 455 CTAG C^TAG
560 CTAG
BsaBI 630 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1482 GATGACTATC
BsaHI 289 GACGCC G[GA]^CG[CT]C
679 GACGCC
BsgI 1075 CTGCAC GTGCAG(16/14)
754 GTGCAG
BsmAI 1417 GAGAC GTCTC(1/5)
595 GTCTC
BsrFI 125 GCCGGC [GA]^CCGG[CT]
214 GCCGGC
BstXI 983 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1346 CCAAAGCTGTGG
Cfr10I 125 GCCGGC [GA]^CCGG[CT]
214 GCCGGC
DsaI 551 CCACGG C^C[GA][CT]GG
1151 CCATGG
EaeI 80 CGGCCG [CT]^GGCC[GA]
1238 TGGCCA
EcoNI 324 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
702 CCTGGACGAGG
EcoT14I 1019 CCAAGG C^C[AT][AT]GG
1151 CCATGG
HaeII 15 GGCGCT [GA]GCGC^[CT]
723 GGCGCT
HgaI 289 GACGC GACGC(5/10)
679 GACGC
Hin1I 289 GACGCC G[GA]^CG[CT]C
679 GACGCC
MaeI 455 CTAG C^TAG
560 CTAG
MamI 630 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1482 GATGACTATC
NaeI 125 GCCGGC GCC^GGC
214 GCCGGC
NgoMIV 125 GCCGGC G^CCGGC
214 GCCGGC
PpuMI 195 GGGACCC [GA]G^G[AT]CC[CT]
442 GGGTCCT
PvuII 659 CAGCTG CAG^CTG
1096 CAGCTG
StyI 1019 CCAAGG C^C[AT][AT]GG
1151 CCATGG
Tsp45I 563 GTGAC ^GT[GC]AC
612 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp509I TspRI TthHB8I Van91I
XhoII