Restriction map for
pF1KB5177
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AciI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BcgI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tth111I Van91I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 162 GTATAC GT^[AC][GT]AC
AclI 494 AACGTT AA^CGTT
AseI 244 ATTAAT AT^TAAT
AsnI 244 ATTAAT AT^TAAT
Asp700I 211 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 9 CCCGGG C^[CT]CG[GA]G
BanI 48 GGTGCC G^G[CT][GA]CC
BbvCI 143 CCTCAGC CCTCAGC(-5/-2)
BbvI 355 GCAGC GCAGC(8/12)
BclI 393 TGATCA T^GATCA
BmyI 636 GGGCAC G[AGT]GC[ACT]^C
BsaBI 304 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 584 GAGACC GGTCTC(1/5)
BsaJI 9 CCCGGG C^C[ACGT][ACGT]GG
BsmAI 584 GAGAC GTCTC(1/5)
BsmFI 66 GGGAC GGGAC(10/14)
BsoBI 9 CCCGGG C^[CT]CG[GA]G
Bsp1286I 636 GGGCAC G[AGT]GC[ACT]^C
BspMI 277 ACCTGC ACCTGC(4/8)
BsrI 73 CCAGT ACTGG(1/-1)
Bst1107I 162 GTATAC GTA^TAC
BstZ17I 162 GTATAC GTA^TAC
DdeI 144 CTCAG C^T[ACGT]AG
EcoT22I 335 ATGCAT ATGCA^T
FbaI 393 TGATCA T^GATCA
Fnu4HI 355 GCAGC GC^[ACGT]GC
HapII 10 CCGG C^CGG
HgaI 432 GCGTC GACGC(5/10)
HindIII 550 AAGCTT A^AGCTT
HpaII 10 CCGG C^CGG
ItaI 355 GCAGC GC^[ACGT]GC
MaeII 495 ACGT A^CGT
MaeIII 152 GTGAC ^GT[ACGT]AC
MamI 304 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 211 GAAGA GAAGA(8/7)
MslI 42 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 146 CAGCTG C[AC]G^C[GT]G
MspI 10 CCGG C^CGG
NlaIV 48 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 335 ATGCAT ATGCA^T
PshBI 244 ATTAAT AT^TAAT
Psp1406I 494 AACGTT AA^CGTT
PstI 279 CTGCAG CTGCA^G
PvuII 146 CAGCTG CAG^CTG
SfaNI 205 GATGC GCATC(5/9)
SfcI 279 CTGCAG C^T[GA][CT]AG
SmaI 9 CCCGGG CCC^GGG
TseI 355 GCAGC G^C[AT]GC
Tsp45I 152 GTGAC ^GT[GC]AC
XcmI 440 CCATTGGTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
XmnI 211 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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BciVI 13 GGATAC GTATCC(6/5)
109 GGATAC
BcnI 9 CCCGG CC^[GC]GG
10 CCGGG
BseRI 100 CTCCTC GAGGAG(10/8)
20 GAGGAG
BsiYI 10 CCGGGATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
79 CCAGAAACAGG
BslI 10 CCGGGATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
79 CCAGAAACAGG
BspHI 383 TCATGA T^CATGA
423 TCATGA
BstF5I 192 GGATG GGATG(2/0)
204 GGATG
BstNI 52 CCAGG CC^[AT]GG
368 CCTGG
Cac8I 120 GCCAGC GC[ACGT]^[ACGT]GC
538 GCTTGC
Cfr13I 88 GGCCC G^G[ACGT]CC
118 GGGCC
DpnII 310 GATC ^GATC
394 GATC
EcoRII 52 CCAGG ^CC[AT]GG
368 CCTGG
FokI 192 GGATG GGATG(9/13)
204 GGATG
HinfI 154 GACTC G^A[ACGT]TC
465 GAGTC
HphI 151 GGTGA GGTGA(8/7)
196 GGTGA
MboI 310 GATC ^GATC
394 GATC
MvaI 52 CCAGG CC^[AT]GG
368 CCTGG
NciI 9 CCCGG CC^[GC]GG
10 CCGGG
NdeII 310 GATC ^GATC
394 GATC
PleI 154 GACTC GAGTC(4/5)
465 GAGTC
RcaI 383 TCATGA T^CATGA
423 TCATGA
Sau3AI 310 GATC ^GATC
394 GATC
Sau96I 88 GGCCC G^G[ACGT]CC
118 GGGCC
TaqI 104 TCGA T^CGA
555 TCGA
TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^
174 CAGTG
TthHB8I 104 TCGA T^CGA
555 TCGA
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AcsI AluI ApoI BsmI BssKI
HaeIII MnlI MseI MwoI NlaIII
ScrFI Tru9I Tsp509I