Restriction map for
pF1KB5179
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AvaII AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KspI MaeI MluI MroI MslI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1006 GGTACC G^GTACC
AccI 868 GTCGAC GT^[AC][GT]AC
AcsI 1053 GAATTT [GA]^AATT[CT]
AfaI 1007 GTAC GT^AC
AocI 763 CCTCAGG CC^T[ACGT]AGG
ApoI 1053 GAATTT [GA]^AATT[CT]
Asp700I 1050 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1006 GGTACC G^GTACC
AspHI 824 GTGCTC G[AT]GC[AT]^C
BalI 1131 TGGCCA TGG^CCA
BamHI 144 GGATCC G^GATCC
BbsI 382 GAAGAC GAAGAC(2/6)
BciVI 714 GGATAC GTATCC(6/5)
BpmI 395 CTGGAG CTGGAG(16/14)
BpuAI 382 GAAGAC GAAGAC(2/6)
BsaBI 1087 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 556 GTGCAG GTGCAG(16/14)
BsiHKAI 824 GTGCTC G[AT]GC[AT]^C
BsmFI 1145 GGGAC GGGAC(10/14)
BssSI 25 CACGAG CACGAG(-5/-1)
BstAPI 558 GCAGTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 458 CCATTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 763 CCTCAGG CC^T[ACGT]AGG
Cac8I 693 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 760 GGGCC G^G[ACGT]CC
DsaI 1156 CCATGG C^C[GA][CT]GG
EaeI 1131 TGGCCA [CT]^GGCC[GA]
Eco81I 763 CCTCAGG CC^T[ACGT]AGG
HapII 933 CCGG C^CGG
HgaI 788 GACGC GACGC(5/10)
HindIII 1174 AAGCTT A^AGCTT
HpaII 933 CCGG C^CGG
KpnI 1006 GGTACC GGTAC^C
MaeII 314 ACGT A^CGT
MamI 1087 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 605 CAATTG C^AATTG
MluNI 1131 TGGCCA TGG^CCA
MscI 1131 TGGCCA TGG^CCA
MspA1I 878 CAGCTG C[AC]G^C[GT]G
MspI 933 CCGG C^CGG
MunI 605 CAATTG C^AATTG
NcoI 1156 CCATGG C^CATGG
PflMI 237 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1121 CTGCAG CTGCA^G
PvuII 878 CAGCTG CAG^CTG
RsaI 1007 GTAC GT^AC
SalI 868 GTCGAC G^TCGAC
Sau96I 760 GGGCC G^G[ACGT]CC
TspRI 111 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 237 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1050 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 1065 CCGC CCGC(-3/-1)
815 GCGG
BanI 838 GGTGCC G^G[CT][GA]CC
1006 GGTACC
BglI 484 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
503 GCCGTAATGGC
BsaI 398 GAGACC GGTCTC(1/5)
951 GGTCTC
BsiEI 71 CGGTCG CG[GA][CT]^CG
870 CGACCG
BsmAI 398 GAGAC GTCTC(1/5)
952 GTCTC
BssKI 402 CCAGG ^CC[ACGT]GG
1069 CCTGG
BstNI 402 CCAGG CC^[AT]GG
1069 CCTGG
EarI 631 GAAGAG CTCTTC(1/4)
1017 GAAGAG
EcoNI 135 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
705 CCTTGATGAGG
EcoRII 402 CCAGG ^CC[AT]GG
1069 CCTGG
HincII 229 GTTGAC GT[CT]^[GA]AC
868 GTCGAC
HindII 229 GTTGAC GT[CT]^[GA]AC
868 GTCGAC
Ksp632I 631 GAAGAG CTCTTC(1/4)
1017 GAAGAG
MvaI 402 CCAGG CC^[AT]GG
1069 CCTGG
PleI 67 GAGTC GAGTC(4/5)
596 GAGTC
ScrFI 402 CCAGG CC^[ACGT]GG
1069 CCTGG
SfcI 730 CTACAG C^T[GA][CT]AG
1121 CTGCAG
SmlI 353 CTCAAG C^T[CT][GA]AG
545 CTCAAG
TfiI 280 GATTC G^A[AT]TC
535 GATTC
Tsp45I 101 GTGAC ^GT[GC]AC
339 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BstF5I BstYI DdeI DpnII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MwoI NdeII NlaIII
NlaIV Sau3AI SfaNI StyI TaqI
Tru9I TseI Tsp509I TthHB8I XhoII