##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1286I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MunI        MvnI        MwoI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        Tru9I       TseI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI           21  CCGC                 CCGC(-3/-1)
AcsI          116  AAATTC               [GA]^AATT[CT]
AfaI          141  GTAC                 GT^AC
ApoI          116  AAATTC               [GA]^AATT[CT]
AvaI           88  CCCGGG               C^[CT]CG[GA]G
BsaWI         153  ACCGGA               [AT]^CCGG[AT]
BsiYI          87  CCCCGGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           87  CCCCGGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         355  GGGAC                GGGAC(10/14)
BsoBI          88  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      140  TGTACA               T^GTACA
BspHI          35  TCATGA               T^CATGA
BsrGI         140  TGTACA               T^GTACA
BsrI          208  ACTGG                ACTGG(1/-1)
BstF5I        156  GGATG                GGATG(2/0)
BstNI         234  CCTGG                CC^[AT]GG
Cac8I         379  GCTGGC               GC[ACGT]^[ACGT]GC
DdeI          107  CTAAG                C^T[ACGT]AG
EcoRII        234  CCTGG                ^CC[AT]GG
FokI          156  GGATG                GGATG(9/13)
HphI           84  TCACC                GGTGA(8/7)
MaeIII         13  GTAAC                ^GT[ACGT]AC
MslI          187  CATTTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          234  CCTGG                CC^[AT]GG
RcaI           35  TCATGA               T^CATGA
RsaI          141  GTAC                 GT^AC
SmaI           88  CCCGGG               CCC^GGG
SmlI          306  CTCAAG               C^T[CT][GA]AG
SspBI         140  TGTACA               T^GTACA
Tsp509I       117  AATT                 ^AATT
TspRI         101  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmaCI          88  CCCGGG               C^CCGGG
XmaI           88  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BcnI           88  CCCGG                CC^[GC]GG
               89  CCGGG               
BfaI           26  CTAG                 C^TAG
              130  CTAG                
HaeIII        283  GGCC                 GG^CC
              366  GGCC                
HapII          89  CCGG                 C^CGG
              154  CCGG                
HinfI         176  GATTC                G^A[ACGT]TC
              229  GATTC               
HpaII          89  CCGG                 C^CGG
              154  CCGG                
MaeI           26  CTAG                 C^TAG
              130  CTAG                
MboII         294  GAAGA                GAAGA(8/7)
              342  GAAGA               
MnlI          305  CCTC                 CCTC(7/6)
               57  GAGG                
MspI           89  CCGG                 C^CGG
              154  CCGG                
NciI           88  CCCGG                CC^[GC]GG
               89  CCGGG               
TfiI          176  GATTC                G^A[AT]TC
              229  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BsaJI       BssKI       NlaIII      ScrFI