Restriction map for
pF1KB5183
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbeI BbrPI BbsI BcgI
BciVI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 562 GTCTAC GT^[AC][GT]AC
AhdI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1164 GTGCAC G^TGCAC
ApaI 20 GGGCCC GGGCC^C
ApaLI 1164 GTGCAC G^TGCAC
AspEI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1164 GTGCAC G[AT]GC[AT]^C
AspI 844 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 23 CCCGGG C^[CT]CG[GA]G
BalI 488 TGGCCA TGG^CCA
BamHI 935 GGATCC G^GATCC
BanI 1186 GGTGCC G^G[CT][GA]CC
BanII 20 GGGCCC G[GA]GC[CT]^C
BbvCI 144 GCTGAGG CCTCAGC(-5/-2)
BclI 455 TGATCA T^GATCA
BfaI 550 CTAG C^TAG
BglII 14 AGATCT A^GATCT
BsgI 554 GTGCAG GTGCAG(16/14)
BsiHKAI 1164 GTGCAC G[AT]GC[AT]^C
BsoBI 23 CCCGGG C^[CT]CG[GA]G
BsrFI 441 GCCGGC [GA]^CCGG[CT]
CfoI 220 GCGC GCG^C
Cfr10I 441 GCCGGC [GA]^CCGG[CT]
DsaI 42 CCGCGG C^C[GA][CT]GG
Eam1105I 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1243 GAAGAG CTCTTC(1/4)
EcoT14I 668 CCTTGG C^C[AT][AT]GG
FbaI 455 TGATCA T^GATCA
HhaI 220 GCGC GCG^C
HinP1I 220 GCGC G^CGC
HincII 1030 GTCAAC GT[CT]^[GA]AC
HindII 1030 GTCAAC GT[CT]^[GA]AC
Ksp632I 1243 GAAGAG CTCTTC(1/4)
KspI 42 CCGCGG CCGC^GG
MaeI 550 CTAG C^TAG
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MslI 612 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 441 GCCGGC GCC^GGC
NgoMIV 441 GCCGGC G^CCGGC
PflFI 844 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 939 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 878 AGGACCC [GA]G^G[AT]CC[CT]
PstI 98 CTGCAG CTGCA^G
SacII 42 CCGCGG CCGC^GG
SapI 1243 GAAGAGC GCTCTTC(1/4)
SexAI 1148 ACCTGGT A^CC[AT]GGT
SfcI 98 CTGCAG C^T[GA][CT]AG
SmaI 23 CCCGGG CCC^GGG
StuI 1004 AGGCCT AGG^CCT
StyI 668 CCTTGG C^C[AT][AT]GG
TaqI 692 TCGA T^CGA
Tsp509I 1236 AATT ^AATT
Tth111I 844 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 692 TCGA T^CGA
Van91I 939 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 43 CGCG CG^CG
219 CGCG
BlpI 370 GCTGAGC GC^T[ACGT]AGC
403 GCTCAGC
Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC
403 GCTCAGC
BsaI 241 GGTCTC GGTCTC(1/5)
867 GGTCTC
BspHI 503 TCATGA T^CATGA
1229 TCATGA
BsrBI 373 GAGCGG CCGCTC(-3/-3)
985 GAGCGG
BsrDI 399 CATTGC GCAATG(2/0)
1140 CATTGC
BstUI 43 CGCG CG^CG
219 CGCG
CelII 370 GCTGAGC GC^T[ACGT]AGC
403 GCTCAGC
DraII 134 AGGCCCT [GA]G^G[ACGT]CC[CT]
878 AGGACCC
EcoO109I 134 AGGCCCT [GA]G^G[ACGT]CC[CT]
878 AGGACCC
MvnI 43 CGCG CG^CG
219 CGCG
RcaI 503 TCATGA T^CATGA
1229 TCATGA
SmlI 574 CTCAAG C^T[CT][GA]AG
1024 CTCAAG
Tsp45I 964 GTGAC ^GT[GC]AC
1048 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
TseI TspRI XcmI XhoII