##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BcgI        BciVI       BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

BbsI          184  GTCTTC               GAAGAC(2/6)
BbvCI         594  GCTGAGG              CCTCAGC(-5/-2)
BclI          248  TGATCA               T^GATCA
BmyI          101  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         184  GTCTTC               GAAGAC(2/6)
BsgI           71  GTGCAG               GTGCAG(16/14)
BsmAI         270  GTCTC                GTCTC(1/5)
Bsp1286I      101  GGGCAC               G[AGT]GC[ACT]^C
BsrDI         303  CATTGC               GCAATG(2/0)
BsrI          160  CCAGT                ACTGG(1/-1)
BssSI         679  CTCGTG               CACGAG(-5/-1)
BstAPI        298  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        413  GGATG                GGATG(2/0)
CfoI           65  GCGC                 GCG^C
Cfr13I        221  GGGCC                G^G[ACGT]CC
DraII         220  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI           89  CCGTGG               C^C[GA][CT]GG
EaeI          293  TGGCCG               [CT]^GGCC[GA]
EcoO109I      220  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       630  CCTTGG               C^C[AT][AT]GG
FauI          218  GCGGG                CCCGC(4/6)
FbaI          248  TGATCA               T^GATCA
FokI          413  GGATG                GGATG(9/13)
HapII         357  CCGG                 C^CGG
HhaI           65  GCGC                 GCG^C
HinP1I         65  GCGC                 G^CGC
HpaII         357  CCGG                 C^CGG
MaeIII        517  GTTAC                ^GT[ACGT]AC
MspI          357  CCGG                 C^CGG
NlaIV         220  GGGGCC               GG[ACGT]^[ACGT]CC
PstI          620  CTGCAG               CTGCA^G
Sau96I        221  GGGCC                G^G[ACGT]CC
SfaNI         414  GATGC                GCATC(5/9)
SfcI          620  CTGCAG               C^T[GA][CT]AG
StyI          630  CCTTGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI          107  AGCT                 AG^CT
              618  AGCT                
AlwI          402  GATCC                GGATC(4/5)
              555  GATCC               
AseI          511  ATTAAT               AT^TAAT
              535  ATTAAT              
AsnI          511  ATTAAT               AT^TAAT
              535  ATTAAT              
BsmFI          78  GGGAC                GGGAC(10/14)
              493  GTCCC               
DdeI          115  CTGAG                C^T[ACGT]AG
              595  CTGAG               
EarI          190  CTCTTC               CTCTTC(1/4)
              684  GAAGAG              
HphI          330  GGTGA                GGTGA(8/7)
              708  GGTGA               
Ksp632I       190  CTCTTC               CTCTTC(1/4)
              684  GAAGAG              
MaeII          44  ACGT                 A^CGT
              253  ACGT                
MslI          171  CATCGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              279  CATGTTGGTG          
NlaIII        279  CATG                 CATG^
              477  CATG                
PshBI         511  ATTAAT               AT^TAAT
              535  ATTAAT              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstNI       Cac8I       DpnII       EcoRII      
Fnu4HI      HaeIII      HinfI       ItaI        MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       PleI        Sau3AI      ScrFI       TaqI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I