Restriction map for
pF1KB5236
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MaeII MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 586 GACGCC G[GA]^CG[CT]C
AfaI 582 GTAC GT^AC
AspHI 848 GTGCTC G[AT]GC[AT]^C
BanII 670 GAGCCC G[GA]GC[CT]^C
BciVI 295 GGATAC GTATCC(6/5)
BclI 45 TGATCA T^GATCA
BcnI 697 CCCGG CC^[GC]GG
BfaI 956 CTAG C^TAG
BglI 960 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 27 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 586 GACGCC G[GA]^CG[CT]C
BsaI 194 GAGACC GGTCTC(1/5)
BsiHKAI 848 GTGCTC G[AT]GC[AT]^C
BspHI 75 TCATGA T^CATGA
BsrFI 619 GCCGGC [GA]^CCGG[CT]
BssSI 523 CACGAG CACGAG(-5/-1)
Cfr10I 619 GCCGGC [GA]^CCGG[CT]
DsaI 290 CCATGG C^C[GA][CT]GG
EaeI 774 TGGCCG [CT]^GGCC[GA]
EarI 3 GAAGAG CTCTTC(1/4)
FbaI 45 TGATCA T^GATCA
Hin1I 586 GACGCC G[GA]^CG[CT]C
HindIII 830 AAGCTT A^AGCTT
HphI 420 GGTGA GGTGA(8/7)
Ksp632I 3 GAAGAG CTCTTC(1/4)
MaeI 956 CTAG C^TAG
MaeIII 169 GTTAC ^GT[ACGT]AC
MamI 27 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 236 TTAA T^TAA
NaeI 619 GCCGGC GCC^GGC
NciI 697 CCCGG CC^[GC]GG
NcoI 290 CCATGG C^CATGG
NgoMIV 619 GCCGGC G^CCGGC
NlaIV 109 GGCCCC GG[ACGT]^[ACGT]CC
PvuII 577 CAGCTG CAG^CTG
RcaI 75 TCATGA T^CATGA
RsaI 582 GTAC GT^AC
SmlI 265 CTCAAG C^T[CT][GA]AG
TfiI 218 GAATC G^A[AT]TC
Tru9I 236 TTAA T^TAA
XcmI 96 CCAAGCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 8 GTCTAC GT^[AC][GT]AC
1039 GTCTAC
AcsI 184 AAATTT [GA]^AATT[CT]
890 AAATTC
AlwI 792 GATCC GGATC(4/5)
72 GGATC
ApoI 184 AAATTT [GA]^AATT[CT]
890 AAATTC
AvaII 242 GGACC G^G[AT]CC
546 GGACC
BsmAI 194 GAGAC GTCTC(1/5)
836 GTCTC
BsrDI 164 GCAATG GCAATG(2/0)
614 GCAATG
EcoT14I 140 CCAAGG C^C[AT][AT]GG
290 CCATGG
HaeII 503 GGCGCT [GA]GCGC^[CT]
968 GGCGCT
HapII 620 CCGG C^CGG
698 CCGG
HgaI 586 GACGC GACGC(5/10)
938 GCGTC
HpaII 620 CCGG C^CGG
698 CCGG
MslI 154 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
173 CACTGCCATG
MspA1I 577 CAGCTG C[AC]G^C[GT]G
906 CCGCTG
MspI 620 CCGG C^CGG
698 CCGG
PleI 6 GAGTC GAGTC(4/5)
701 GAGTC
PstI 972 CTGCAG CTGCA^G
989 CTGCAG
StyI 140 CCAAGG C^C[AT][AT]GG
290 CCATGG
TaqI 192 TCGA T^CGA
572 TCGA
TthHB8I 192 TCGA T^CGA
572 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp509I
TspRI