Restriction map for
pF1KB5238
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MaeI MfeI
MflI MluI MroI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 535 GACGTC GACGT^C
AfaI 144 GTAC GT^AC
Alw44I 243 GTGCAC G^TGCAC
AlwI 294 GATCC GGATC(4/5)
ApaLI 243 GTGCAC G^TGCAC
Asp700I 255 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 243 GTGCAC G[AT]GC[AT]^C
AvaII 436 GGTCC G^G[AT]CC
BalI 609 TGGCCA TGG^CCA
BbrPI 400 CACGTG CAC^GTG
BcgI 530 GCATGGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 128 CCGGG CC^[GC]GG
BglI 311 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 190 CTGGAG CTGGAG(16/14)
BsaAI 400 CACGTG [CT]AC^GT[GA]
BsaBI 591 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 468 CTGCAC GTGCAG(16/14)
BsiEI 177 CGGCCG CG[GA][CT]^CG
BsiHKAI 243 GTGCAC G[AT]GC[AT]^C
BssSI 139 CTCGTG CACGAG(-5/-1)
DpnII 294 GATC ^GATC
DraIII 286 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 177 CGGCCG C^GGCCG
EarI 114 CTCTTC CTCTTC(1/4)
EclXI 177 CGGCCG C^GGCCG
Eco52I 177 CGGCCG C^GGCCG
EcoNI 281 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 422 CCATGG C^C[AT][AT]GG
HaeII 6 GGCGCT [GA]GCGC^[CT]
HgaI 157 GACGC GACGC(5/10)
HinfI 553 GAATC G^A[ACGT]TC
Ksp632I 114 CTCTTC CTCTTC(1/4)
KspI 74 CCGCGG CCGC^GG
MaeIII 515 GTCAC ^GT[ACGT]AC
MamI 591 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 294 GATC ^GATC
MluNI 609 TGGCCA TGG^CCA
MscI 609 TGGCCA TGG^CCA
NciI 128 CCGGG CC^[GC]GG
NcoI 422 CCATGG C^CATGG
NdeII 294 GATC ^GATC
NspI 501 GCATGT [GA]CATG^[CT]
PmaCI 400 CACGTG CAC^GTG
PmlI 400 CACGTG CAC^GTG
PstI 87 CTGCAG CTGCA^G
PvuII 221 CAGCTG CAG^CTG
RsaI 144 GTAC GT^AC
SacII 74 CCGCGG CCGC^GG
Sau3AI 294 GATC ^GATC
SfcI 87 CTGCAG C^T[GA][CT]AG
StyI 422 CCATGG C^C[AT][AT]GG
TfiI 553 GAATC G^A[AT]TC
Tsp45I 515 GTCAC ^GT[GC]AC
XmnI 255 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 157 GACGCC G[GA]^CG[CT]C
535 GACGTC
AluI 194 AGCT AG^CT
222 AGCT
BanII 96 GGGCTC G[GA]GC[CT]^C
236 GAGCCC
BsaHI 157 GACGCC G[GA]^CG[CT]C
535 GACGTC
BsaJI 74 CCGCGG C^C[ACGT][ACGT]GG
422 CCATGG
BseRI 120 CTCCTC GAGGAG(10/8)
123 CTCCTC
BsmFI 46 GTCCC GGGAC(10/14)
211 GTCCC
BsrBI 136 CCGCTC CCGCTC(-3/-3)
172 CCGCTC
BstF5I 581 CATCC GGATG(2/0)
492 GGATG
DdeI 224 CTGAG C^T[ACGT]AG
566 CTAAG
DsaI 74 CCGCGG C^C[GA][CT]GG
422 CCATGG
EaeI 177 CGGCCG [CT]^GGCC[GA]
609 TGGCCA
FokI 581 CATCC GGATG(9/13)
492 GGATG
HapII 101 CCGG C^CGG
128 CCGG
Hin1I 157 GACGCC G[GA]^CG[CT]C
535 GACGTC
HpaII 101 CCGG C^CGG
128 CCGG
MspI 101 CCGG C^CGG
128 CCGG
TspRI 246 CACTG CA[GC]TG[ACGT][ACGT]^
392 CACTG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI BbvI BmyI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HphI ItaI MaeII MboII MnlI
MspA1I MvaI MvnI MwoI NlaIII
NlaIV Sau96I ScrFI TseI