Restriction map for
pF1KB5271
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BseRI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BstAPI BstEII
BstPI BstXI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MscI MspA1I MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tsp45I Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1148 GTATAC GT^[AC][GT]AC
AccII 635 CGCG CG^CG
AccIII 606 TCCGGA T^CCGGA
AlwI 778 GGATC GGATC(4/5)
AseI 397 ATTAAT AT^TAAT
AsnI 397 ATTAAT AT^TAAT
Asp700I 395 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 485 GAGCAC G[AT]GC[AT]^C
AvrII 495 CCTAGG C^CTAGG
BbsI 36 GAAGAC GAAGAC(2/6)
BbvI 682 GCAGC GCAGC(8/12)
BlnI 495 CCTAGG C^CTAGG
BmyI 485 GAGCAC G[AGT]GC[ACT]^C
BpmI 89 CTGGAG CTGGAG(16/14)
BpuAI 36 GAAGAC GAAGAC(2/6)
BsaI 254 GAGACC GGTCTC(1/5)
BsaWI 606 TCCGGA [AT]^CCGG[AT]
BseAI 606 TCCGGA T^CCGGA
BsiEI 44 CGGTCG CG[GA][CT]^CG
BsiHKAI 485 GAGCAC G[AT]GC[AT]^C
BsmFI 716 GTCCC GGGAC(10/14)
Bsp1286I 485 GAGCAC G[AGT]GC[ACT]^C
BspEI 606 TCCGGA T^CCGGA
BspMI 1038 ACCTGC ACCTGC(4/8)
BssSI 317 CTCGTG CACGAG(-5/-1)
Bst1107I 1148 GTATAC GTA^TAC
BstBI 1022 TTCGAA TT^CGAA
BstUI 635 CGCG CG^CG
BstZ17I 1148 GTATAC GTA^TAC
CfoI 634 GCGC GCG^C
DraII 14 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 407 CCATGG C^C[GA][CT]GG
EcoNI 57 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 14 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 29 GCGGG CCCGC(4/6)
HapII 607 CCGG C^CGG
HhaI 634 GCGC GCG^C
HinP1I 634 GCGC G^CGC
HpaII 607 CCGG C^CGG
HphI 243 GGTGA GGTGA(8/7)
MaeIII 444 GTTAC ^GT[ACGT]AC
MroI 606 TCCGGA T^CCGGA
MslI 924 CACTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 607 CCGG C^CGG
MvnI 635 CGCG CG^CG
MwoI 17 GCCTGGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 407 CCATGG C^CATGG
NlaIII 408 CATG CATG^
NspV 1022 TTCGAA TT^CGAA
PflMI 745 CCATTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 39 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 397 ATTAAT AT^TAAT
SfuI 1022 TTCGAA TT^CGAA
StuI 65 AGGCCT AGG^CCT
TseI 682 GCAGC G^C[AT]GC
Van91I 745 CCATTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 842 CCACTTTAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 395 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 548 GTAC GT^AC
638 GTAC
AvaII 100 GGTCC G^G[AT]CC
411 GGTCC
BsmAI 254 GAGAC GTCTC(1/5)
307 GAGAC
BsrBI 4 CCGCTC CCGCTC(-3/-3)
24 GAGCGG
BsrI 97 ACTGG ACTGG(1/-1)
466 CCAGT
BstF5I 213 CATCC GGATG(2/0)
140 GGATG
Cac8I 357 GCTTGC GC[ACGT]^[ACGT]GC
957 GCTTGC
EcoRI 208 GAATTC G^AATTC
1025 GAATTC
FokI 213 CATCC GGATG(9/13)
140 GGATG
HgaI 387 GACGC GACGC(5/10)
714 GCGTC
HincII 553 GTTGAC GT[CT]^[GA]AC
736 GTTGAC
HindII 553 GTTGAC GT[CT]^[GA]AC
736 GTTGAC
NlaIV 342 GGTTCC GG[ACGT]^[ACGT]CC
1106 GGAGCC
RsaI 548 GTAC GT^AC
638 GTAC
SfaNI 141 GATGC GCATC(5/9)
234 GCATC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BsaJI BsiYI BslI BssKI BstNI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MvaI NdeII SapI Sau3AI
Sau96I ScrFI SspI StyI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I