Restriction map for
pF1KA0292
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII AvrII BamHI BbrPI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BssHII BssSI CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco52I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SgrAI SnaBI SpeI
SphI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1326 GGTACC G^GTACC
AccI 990 GTATAC GT^[AC][GT]AC
AflIII 1415 ACATGT A^C[GA][CT]GT
AhdI 2621 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5631 AGCGCT AGC^GCT
Asp718I 1326 GGTACC G^GTACC
AspEI 2621 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 3 GCAGTCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2178 GTATCC GTATCC(6/5)
BsaAI 4273 CACGTA [CT]AC^GT[GA]
BsmBI 2695 CGTCTC CGTCTC(1/5)
Bsp1407I 4939 TGTACA T^GTACA
BspLU11I 1415 ACATGT A^CATGT
BspMI 3229 GCAGGT ACCTGC(4/8)
BsrFI 1677 ACCGGC [GA]^CCGG[CT]
BsrGI 4939 TGTACA T^GTACA
Bst1107I 990 GTATAC GTA^TAC
BstBI 4107 TTCGAA TT^CGAA
BstZ17I 990 GTATAC GTA^TAC
Cfr10I 1677 ACCGGC [GA]^CCGG[CT]
Eam1105I 2621 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5631 AGCGCT AGC^GCT
EcoRI 3237 GAATTC G^AATTC
EcoRV 3919 GATATC GAT^ATC
EcoT22I 1946 ATGCAT ATGCA^T
KpnI 1326 GGTACC GGTAC^C
KspI 2883 CCGCGG CCGC^GG
MaeII 4274 ACGT A^CGT
NsiI 1946 ATGCAT ATGCA^T
NspV 4107 TTCGAA TT^CGAA
PflMI 4854 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 2883 CCGCGG CCGC^GG
SapI 5302 GAAGAGC GCTCTTC(1/4)
SfuI 4107 TTCGAA TT^CGAA
SmaI 1826 CCCGGG CCC^GGG
SspBI 4939 TGTACA T^GTACA
SspI 4087 AATATT AAT^ATT
StuI 328 AGGCCT AGG^CCT
Van91I 4854 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1826 CCCGGG C^CCGGG
XmaI 1826 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccII 2884 CGCG CG^CG
5841 CGCG
AcyI 5363 GGCGCC G[GA]^CG[CT]C
5680 GGCGCC
AocI 1048 CCTGAGG CC^T[ACGT]AGG
4513 CCTGAGG
Asp700I 326 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2263 GAAGTGCTTC
AspI 4243 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5082 GACAGAGTC
AvaI 1826 CCCGGG C^[CT]CG[GA]G
3200 CTCGGG
BbeI 5363 GGCGCC GGCGC^C
5680 GGCGCC
BsaHI 5363 GGCGCC G[GA]^CG[CT]C
5680 GGCGCC
BsaWI 3131 ACCGGA [AT]^CCGG[AT]
5141 ACCGGA
BsgI 1491 CTGCAC GTGCAG(16/14)
343 GTGCAG
BsmI 798 GAATGC GAATGC(1/-1)
3883 GCATTC
BsoBI 1826 CCCGGG C^[CT]CG[GA]G
3200 CTCGGG
BspHI 3027 TCATGA T^CATGA
3755 TCATGA
BstUI 2884 CGCG CG^CG
5841 CGCG
Bsu36I 1048 CCTGAGG CC^T[ACGT]AGG
4513 CCTGAGG
DraIII 823 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
1682 CACAAGGTG
Eco81I 1048 CCTGAGG CC^T[ACGT]AGG
4513 CCTGAGG
Hin1I 5363 GGCGCC G[GA]^CG[CT]C
5680 GGCGCC
KasI 5363 GGCGCC G^GCGCC
5680 GGCGCC
MvnI 2884 CGCG CG^CG
5841 CGCG
NarI 5363 GGCGCC GG^CGCC
5680 GGCGCC
NcoI 2214 CCATGG C^CATGG
2747 CCATGG
NheI 208 GCTAGC G^CTAGC
2395 GCTAGC
PflFI 4243 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5082 GACAGAGTC
RcaI 3027 TCATGA T^CATGA
3755 TCATGA
SbfI 2256 CCTGCAGG CCTGCA^GG
5622 CCTGCAGG
ScaI 1378 AGTACT AGT^ACT
2311 AGTACT
SfiI 329 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2041 GGCCAGAGTGGCC
SfoI 5363 GGCGCC GGC^GCC
5680 GGCGCC
Sse8387I 2256 CCTGCAGG CCTGCA^GG
5622 CCTGCAGG
TaqI 4108 TCGA T^CGA
5470 TCGA
Tth111I 4243 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5082 GACAGAGTC
TthHB8I 4108 TCGA T^CGA
5470 TCGA
XcmI 3645 CCATGAGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4850 CCACCCAGCCACTGG
XmnI 326 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2263 GAAGTGCTTC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII