Restriction map for
pF1KA0806
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# NO CUT ENZYMES ( 87 enzymes) #
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AccI AccIII AclI AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI AspEI AviII BamHI
BbrPI BcgI BglI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KspI MamI MluI
MroI NdeI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI Sse8387I
SspBI SwaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1972 GACGTC GACGT^C
Acc65I 2689 GGTACC G^GTACC
AccII 132 CGCG CG^CG
Asp718I 2689 GGTACC G^GTACC
AspI 2796 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 15 CCTAGG C^CTAGG
BanII 2533 GAGCTC G[GA]GC[CT]^C
BclI 2790 TGATCA T^GATCA
BlnI 15 CCTAGG C^CTAGG
BsgI 1886 CTGCAC GTGCAG(16/14)
BsmBI 2176 CGTCTC CGTCTC(1/5)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BssSI 827 CACGAG CACGAG(-5/-1)
BstUI 132 CGCG CG^CG
BstXI 2804 CCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
DraI 1302 TTTAAA TTT^AAA
DraIII 1590 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1850 CCATGG C^C[GA][CT]GG
EcoNI 3122 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 914 ATGCAT ATGCA^T
FbaI 2790 TGATCA T^GATCA
KpnI 2689 GGTACC GGTAC^C
MfeI 2361 CAATTG C^AATTG
MunI 2361 CAATTG C^AATTG
MvnI 132 CGCG CG^CG
NaeI 6 GCCGGC GCC^GGC
NcoI 1850 CCATGG C^CATGG
NgoMIV 6 GCCGGC G^CCGGC
NheI 1428 GCTAGC G^CTAGC
NsiI 914 ATGCAT ATGCA^T
PaeR7I 1937 CTCGAG C^TCGAG
PflFI 2796 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2740 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 163 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 964 CAGCTG CAG^CTG
SacI 2533 GAGCTC GAGCT^C
SgrAI 5 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2693 CCCGGG CCC^GGG
SpeI 1684 ACTAGT A^CTAGT
SspI 361 AATATT AAT^ATT
StuI 3012 AGGCCT AGG^CCT
Tth111I 2796 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2376 CCAGAGTAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1937 CTCGAG C^TCGAG
XmaCI 2693 CCCGGG C^CCGGG
XmaI 2693 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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Alw44I 1412 GTGCAC G^TGCAC
1554 GTGCAC
AlwNI 34 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2794 CAGACACTG
ApaLI 1412 GTGCAC G^TGCAC
1554 GTGCAC
Asp700I 1209 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1309 GAACTGATTC
BalI 1853 TGGCCA TGG^CCA
2328 TGGCCA
BbeI 3 GGCGCC GGCGC^C
9 GGCGCC
BbvCI 450 CCTCAGC CCTCAGC(-5/-2)
2773 GCTGAGG
BciVI 1629 GGATAC GTATCC(6/5)
2856 GGATAC
BglII 629 AGATCT A^GATCT
724 AGATCT
BsaI 121 GGTCTC GGTCTC(1/5)
2047 GGTCTC
BspMI 2293 ACCTGC ACCTGC(4/8)
118 GCAGGT
BsrBI 89 CCGCTC CCGCTC(-3/-3)
2964 GAGCGG
BsrDI 78 CATTGC GCAATG(2/0)
2975 CATTGC
BstAPI 1953 GCACGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2634 GCAACTTATGC
DraII 2740 AGGACCC [GA]G^G[ACGT]CC[CT]
3077 AGGGCCT
DrdI 1030 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1151 GACTCACAAGTC
EaeI 1853 TGGCCA [CT]^GGCC[GA]
2328 TGGCCA
EarI 73 CTCTTC CTCTTC(1/4)
2370 CTCTTC
EcoO109I 2740 AGGACCC [GA]G^G[ACGT]CC[CT]
3077 AGGGCCT
HgaI 1946 GACGC GACGC(5/10)
20 GCGTC
KasI 3 GGCGCC G^GCGCC
9 GGCGCC
Ksp632I 73 CTCTTC CTCTTC(1/4)
2370 CTCTTC
MluNI 1853 TGGCCA TGG^CCA
2328 TGGCCA
MscI 1853 TGGCCA TGG^CCA
2328 TGGCCA
NarI 3 GGCGCC GG^CGCC
9 GGCGCC
NspI 497 GCATGC [GA]CATG^[CT]
1949 GCATGC
PflMI 651 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1589 CCACTGTGTGG
SfoI 3 GGCGCC GGC^GCC
9 GGCGCC
SphI 497 GCATGC GCATG^C
1949 GCATGC
Van91I 651 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1589 CCACTGTGTGG
XbaI 52 TCTAGA T^CTAGA
1242 TCTAGA
XmnI 1209 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1309 GAACTGATTC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII