Restriction map for
pF1KA1275
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccII AccIII AflII AflIII
AhdI AscI Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BstEII BstPI
BstUI CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 2400 GACGTC GACGT^C
AclI 2875 AACGTT AA^CGTT
AcyI 2400 GACGTC G[GA]^CG[CT]C
AgeI 3396 ACCGGT A^CCGGT
Alw44I 1344 GTGCAC G^TGCAC
Aor51HI 2339 AGCGCT AGC^GCT
ApaI 2659 GGGCCC GGGCC^C
ApaLI 1344 GTGCAC G^TGCAC
AseI 2199 ATTAAT AT^TAAT
AsnI 2199 ATTAAT AT^TAAT
AspI 3030 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3416 CTCGAG C^[CT]CG[GA]G
BanI 2684 GGCACC G^G[CT][GA]CC
BbvCI 822 GCTGAGG CCTCAGC(-5/-2)
BciVI 2565 GTATCC GTATCC(6/5)
BclI 1980 TGATCA T^GATCA
BcnI 1869 CCCGG CC^[GC]GG
BsaHI 2400 GACGTC G[GA]^CG[CT]C
BsaI 3215 GGTCTC GGTCTC(1/5)
BsaWI 3396 ACCGGT [AT]^CCGG[AT]
BsgI 379 CTGCAC GTGCAG(16/14)
BsmBI 1924 CGTCTC CGTCTC(1/5)
BsmI 3476 GCATTC GAATGC(1/-1)
BsoBI 3416 CTCGAG C^[CT]CG[GA]G
BsrBI 619 GAGCGG CCGCTC(-3/-3)
BsrDI 74 GCAATG GCAATG(2/0)
BssSI 2752 CACGAG CACGAG(-5/-1)
BstAPI 501 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1148 TTCGAA TT^CGAA
EaeI 512 TGGCCG [CT]^GGCC[GA]
Eco47III 2339 AGCGCT AGC^GCT
EcoNI 2827 CCTACCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1980 TGATCA T^GATCA
HaeII 2339 AGCGCT [GA]GCGC^[CT]
Hin1I 2400 GACGTC G[GA]^CG[CT]C
MfeI 1933 CAATTG C^AATTG
MunI 1933 CAATTG C^AATTG
NciI 1869 CCCGG CC^[GC]GG
NspV 1148 TTCGAA TT^CGAA
PaeR7I 3416 CTCGAG C^TCGAG
PflFI 3030 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3396 ACCGGT A^CCGGT
PpuMI 374 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2199 ATTAAT AT^TAAT
Psp1406I 2875 AACGTT AA^CGTT
SfuI 1148 TTCGAA TT^CGAA
SphI 491 GCATGC GCATG^C
SwaI 1358 ATTTAAAT ATTT^AAAT
Tth111I 3030 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2556 TCTAGA T^CTAGA
XhoI 3416 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 291 GATCC GGATC(4/5)
807 GGATC
AvaII 375 GGTCC G^G[AT]CC
3230 GGTCC
BglII 4 AGATCT A^GATCT
2545 AGATCT
Bst1107I 538 GTATAC GTA^TAC
1693 GTATAC
BstXI 602 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2318 CCAAAGAGTTGG
BstZ17I 538 GTATAC GTA^TAC
1693 GTATAC
DraI 1359 TTTAAA TTT^AAA
1662 TTTAAA
HincII 1777 GTTGAC GT[CT]^[GA]AC
2403 GTCAAC
HindII 1777 GTTGAC GT[CT]^[GA]AC
2403 GTCAAC
NcoI 2611 CCATGG C^CATGG
3092 CCATGG
NspI 296 ACATGC [GA]CATG^[CT]
491 GCATGC
PflMI 1929 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2974 CCAGAAAGTGG
PstI 1054 CTGCAG CTGCA^G
1770 CTGCAG
Van91I 1929 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2974 CCAGAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI AocI ApoI Asp700I AspHI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI