Restriction map for
pF1KA1526
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# NO CUT ENZYMES ( 86 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
BamHI BciVI BclI BglII BsaBI
BseAI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I Van91I XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflII 1036 CTTAAG C^TTAAG
AflIII 500 ACGTGT A^C[GA][CT]GT
AocI 771 CCTGAGG CC^T[ACGT]AGG
Aor51HI 132 AGCGCT AGC^GCT
ApaI 1218 GGGCCC GGGCC^C
AviII 224 TGCGCA TGC^GCA
AvrII 2521 CCTAGG C^CTAGG
BalI 2127 TGGCCA TGG^CCA
BbrPI 2584 CACGTG CAC^GTG
BbvCI 1485 GCTGAGG CCTCAGC(-5/-2)
BfrI 1036 CTTAAG C^TTAAG
BlnI 2521 CCTAGG C^CTAGG
BsiWI 114 CGTACG C^GTACG
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BssHII 199 GCGCGC G^CGCGC
BstAPI 807 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 632 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1708 AGATCC [GA]^GATC[CT]
Bsu36I 771 CCTGAGG CC^T[ACGT]AGG
Eco47III 132 AGCGCT AGC^GCT
Eco81I 771 CCTGAGG CC^T[ACGT]AGG
EcoNI 1425 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 224 TGCGCA TGC^GCA
HindIII 1731 AAGCTT A^AGCTT
MflI 1708 AGATCC [GA]^GATC[CT]
MluNI 2127 TGGCCA TGG^CCA
MscI 2127 TGGCCA TGG^CCA
MseI 1037 TTAA T^TAA
NaeI 1225 GCCGGC GCC^GGC
NgoMIV 1225 GCCGGC G^CCGGC
PmaCI 2584 CACGTG CAC^GTG
PmlI 2584 CACGTG CAC^GTG
PstI 2078 CTGCAG CTGCA^G
SacI 30 GAGCTC GAGCT^C
SapI 312 GCTCTTC GCTCTTC(1/4)
StuI 2657 AGGCCT AGG^CCT
Tru9I 1037 TTAA T^TAA
XbaI 978 TCTAGA T^CTAGA
XhoII 1708 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AatII 1072 GACGTC GACGT^C
2200 GACGTC
BbeI 263 GGCGCC GGCGC^C
2497 GGCGCC
BbsI 211 GTCTTC GAAGAC(2/6)
1858 GTCTTC
BcgI 206 GCAACGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2480 GCATCGCCATCG
BlpI 147 GCTGAGC GC^T[ACGT]AGC
2549 GCTCAGC
Bpu1102I 147 GCTGAGC GC^T[ACGT]AGC
2549 GCTCAGC
BpuAI 211 GTCTTC GAAGAC(2/6)
1858 GTCTTC
BsaAI 499 TACGTG [CT]AC^GT[GA]
2584 CACGTG
BsaWI 252 TCCGGT [AT]^CCGG[AT]
791 ACCGGA
BsmBI 1359 CGTCTC CGTCTC(1/5)
1959 CGTCTC
BspMI 344 ACCTGC ACCTGC(4/8)
1295 ACCTGC
BssSI 445 CACGAG CACGAG(-5/-1)
1265 CACGAG
CelII 147 GCTGAGC GC^T[ACGT]AGC
2549 GCTCAGC
DraIII 484 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1477 CACCTGGTG
EarI 313 CTCTTC CTCTTC(1/4)
1938 CTCTTC
HincII 571 GTCAAC GT[CT]^[GA]AC
2014 GTCAAC
HindII 571 GTCAAC GT[CT]^[GA]AC
2014 GTCAAC
KasI 263 GGCGCC G^GCGCC
2497 GGCGCC
Ksp632I 313 CTCTTC CTCTTC(1/4)
1938 CTCTTC
NarI 263 GGCGCC GG^CGCC
2497 GGCGCC
PleI 2274 GACTC GAGTC(4/5)
1501 GAGTC
PpuMI 1814 AGGACCT [GA]G^G[AT]CC[CT]
1912 GGGACCT
PvuII 121 CAGCTG CAG^CTG
307 CAGCTG
SfoI 263 GGCGCC GGC^GCC
2497 GGCGCC
SmaI 590 CCCGGG CCC^GGG
1531 CCCGGG
XmaCI 590 CCCGGG C^CCGGG
1531 CCCGGG
XmaI 590 CCCGGG C^CCGGG
1531 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AspEI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI