Restriction map for
pF1KA1628
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbrPI BcgI
BciVI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EarI EcoNI
EcoRI EcoRV FseI FspI HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SalI SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Asp700I 2439 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3513 GGATCC G^GATCC
BbsI 2416 GAAGAC GAAGAC(2/6)
BclI 2819 TGATCA T^GATCA
BpuAI 2416 GAAGAC GAAGAC(2/6)
BsaAI 1504 TACGTG [CT]AC^GT[GA]
BsmBI 91 GAGACG CGTCTC(1/5)
BsmI 2379 GAATGC GAATGC(1/-1)
Bsp1407I 2651 TGTACA T^GTACA
BsrBI 1348 GAGCGG CCGCTC(-3/-3)
BsrGI 2651 TGTACA T^GTACA
BssSI 2149 CTCGTG CACGAG(-5/-1)
BstAPI 3607 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1968 CCAGATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2476 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 313 GACGAGTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 2730 CCATGG C^C[AT][AT]GG
EcoT22I 1846 ATGCAT ATGCA^T
FbaI 2819 TGATCA T^GATCA
HindIII 412 AAGCTT A^AGCTT
NcoI 2730 CCATGG C^CATGG
NsiI 1846 ATGCAT ATGCA^T
PshAI 399 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2028 CGATCG CGAT^CG
SmlI 2083 CTCAAG C^T[CT][GA]AG
SspBI 2651 TGTACA T^GTACA
StyI 2730 CCATGG C^C[AT][AT]GG
TaqI 1791 TCGA T^CGA
TthHB8I 1791 TCGA T^CGA
XmnI 2439 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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Aor51HI 1092 AGCGCT AGC^GCT
2516 AGCGCT
AspI 727 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2577 GACACCGTC
BglII 1970 AGATCT A^GATCT
3179 AGATCT
BstEII 207 GGTGACC G^GT[ACGT]ACC
2391 GGTCACC
BstPI 207 GGTGACC G^GT[ACGT]ACC
2391 GGTCACC
Eco47III 1092 AGCGCT AGC^GCT
2516 AGCGCT
EcoO65I 207 GGTGACC G^GT[ACGT]ACC
2391 GGTCACC
HincII 42 GTTGAC GT[CT]^[GA]AC
2341 GTCAAC
HindII 42 GTTGAC GT[CT]^[GA]AC
2341 GTCAAC
KspI 21 CCGCGG CCGC^GG
1084 CCGCGG
PflFI 727 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2577 GACACCGTC
SacI 982 GAGCTC GAGCT^C
3011 GAGCTC
SacII 21 CCGCGG CCGC^GG
1084 CCGCGG
SexAI 536 ACCAGGT A^CC[AT]GGT
3380 ACCAGGT
StuI 2964 AGGCCT AGG^CCT
3717 AGGCCT
Tsp509I 894 AATT ^AATT
3050 AATT
Tth111I 727 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2577 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfoI SmaI Sse8387I
TfiI TseI Tsp45I TspRI Van91I
XcmI XhoII XmaCI XmaI