Restriction map for
pF1KA1643
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# NO CUT ENZYMES ( 75 enzymes) #
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AccIII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI AviII
AvrII BbrPI BciVI BclI BfaI
BfrI BlnI BsaBI BseAI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI DraI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HpaI
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tsp509I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 490 GACGTC GACGT^C
Acc65I 623 GGTACC G^GTACC
AccI 1154 GTAGAC GT^[AC][GT]AC
AhdI 1057 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 474 AGCGCT AGC^GCT
Asp718I 623 GGTACC G^GTACC
AspEI 1057 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2373 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 42 GGATCC G^GATCC
BbvCI 477 GCTGAGG CCTCAGC(-5/-2)
BcgI 1934 CGAGTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2528 AGATCT A^GATCT
BsaAI 562 TACGTG [CT]AC^GT[GA]
BsaI 2371 GAGACC GGTCTC(1/5)
BsiEI 1586 CGGTCG CG[GA][CT]^CG
BsiWI 1778 CGTACG C^GTACG
BsmI 1680 GCATTC GAATGC(1/-1)
BspHI 338 TCATGA T^CATGA
BsrDI 1086 CATTGC GCAATG(2/0)
BstEII 2319 GGTCACC G^GT[ACGT]ACC
BstPI 2319 GGTCACC G^GT[ACGT]ACC
CpoI 2591 CGGTCCG CG^G[AT]CCG
DraIII 2466 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1579 GACTCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1057 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 586 CTCTTC CTCTTC(1/4)
Eco47III 474 AGCGCT AGC^GCT
EcoNI 627 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2319 GGTCACC G^GT[ACGT]ACC
HindIII 1648 AAGCTT A^AGCTT
KpnI 623 GGTACC GGTAC^C
Ksp632I 586 CTCTTC CTCTTC(1/4)
KspI 98 CCGCGG CCGC^GG
NcoI 530 CCATGG C^CATGG
PaeR7I 1384 CTCGAG C^TCGAG
PflFI 2373 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 338 TCATGA T^CATGA
RsrII 2591 CGGTCCG CG^G[AT]CCG
SacI 511 GAGCTC GAGCT^C
SacII 98 CCGCGG CCGC^GG
SapI 585 GCTCTTC GCTCTTC(1/4)
ScaI 557 AGTACT AGT^ACT
SexAI 89 ACCAGGT A^CC[AT]GGT
SmaI 1755 CCCGGG CCC^GGG
Tth111I 2373 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 365 CCATGCGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1384 CTCGAG C^TCGAG
XmaCI 1755 CCCGGG C^CCGGG
XmaI 1755 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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ApaI 608 GGGCCC GGGCC^C
1811 GGGCCC
Asp700I 3 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2526 GAAGATCTTC
BbsI 748 GAAGAC GAAGAC(2/6)
2422 GAAGAC
BpuAI 748 GAAGAC GAAGAC(2/6)
2422 GAAGAC
BsaWI 1514 ACCGGA [AT]^CCGG[AT]
2144 TCCGGT
BspLU11I 752 ACATGT A^CATGT
2615 ACATGT
BspMI 1963 GCAGGT ACCTGC(4/8)
2580 GCAGGT
BssSI 2017 CACGAG CACGAG(-5/-1)
703 CTCGTG
BstAPI 2465 GCACGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2603 GCACCCACTGC
BstXI 1261 CCATTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1568 CCACTTCCCTGG
FseI 288 GGCCGGCC GGCCGG^CC
2649 GGCCGGCC
SmlI 1368 CTTGAG C^T[CT][GA]AG
1384 CTCGAG
SphI 1995 GCATGC GCATG^C
2112 GCATGC
StuI 1142 AGGCCT AGG^CCT
2450 AGGCCT
TaqI 488 TCGA T^CGA
1385 TCGA
TthHB8I 488 TCGA T^CGA
1385 TCGA
XmnI 3 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2526 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI Tru9I TseI Tsp45I TspRI
Van91I XhoII