Restriction map for pF1KA1849
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# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AocI        ApoI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BclI        BfrI        
BglII       BlnI        BsaBI       BsiWI       BsmBI       
BsmI        BspDI       BspLU11I    BsrDI       BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FspI        HindIII     HpaI        
KpnI        MamI        MfeI        MluI        MseI        
MunI        NdeI        NheI        NotI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tru9I       
Van91I      XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1705  GTCTAC               GT^[AC][GT]AC
AccIII       2829  TCCGGA               T^CCGGA
Alw44I       1876  GTGCAC               G^TGCAC
Aor51HI      2778  AGCGCT               AGC^GCT
ApaLI        1876  GTGCAC               G^TGCAC
AscI         1101  GGCGCGCC             GG^CGCGCC
AspI          208  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2503  CACGTG               CAC^GTG
BbsI         2914  GTCTTC               GAAGAC(2/6)
BbvCI         474  GCTGAGG              CCTCAGC(-5/-2)
BcgI          561  GCAGCGGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3068  GTATCC               GTATCC(6/5)
BfaI         1426  CTAG                 C^TAG
BlpI           93  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       93  GCTGAGC              GC^T[ACGT]AGC
BpuAI        2914  GTCTTC               GAAGAC(2/6)
BsaI          649  GAGACC               GGTCTC(1/5)
BsaWI        2829  TCCGGA               [AT]^CCGG[AT]
BseAI        2829  TCCGGA               T^CCGGA
Bsp1407I     1727  TGTACA               T^GTACA
BspEI        2829  TCCGGA               T^CCGGA
BspHI        1256  TCATGA               T^CATGA
BsrGI        1727  TGTACA               T^GTACA
BssHII       1102  GCGCGC               G^CGCGC
BstYI        3194  AGATCC               [GA]^GATC[CT]
CelII          93  GCTGAGC              GC^T[ACGT]AGC
Eco47III     2778  AGCGCT               AGC^GCT
FseI          318  GGCCGGCC             GGCCGG^CC
HincII        973  GTCAAC               GT[CT]^[GA]AC
HindII        973  GTCAAC               GT[CT]^[GA]AC
KspI          926  CCGCGG               CCGC^GG
MaeI         1426  CTAG                 C^TAG
MaeIII        338  GTGAC                ^GT[ACGT]AC
MflI         3194  AGATCC               [GA]^GATC[CT]
MroI         2829  TCCGGA               T^CCGGA
NruI         3164  TCGCGA               TCG^CGA
PflFI         208  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2503  CACGTG               CAC^GTG
PmlI         2503  CACGTG               CAC^GTG
RcaI         1256  TCATGA               T^CATGA
SacII         926  CCGCGG               CCGC^GG
SphI           77  GCATGC               GCATG^C
SspBI        1727  TGTACA               T^GTACA
TfiI         1618  GAATC                G^A[AT]TC
Tsp45I        338  GTGAC                ^GT[GC]AC
Tsp509I       434  AATT                 ^AATT
Tth111I       208  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        3194  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsaAI         328  TACGTG               [CT]AC^GT[GA]
             2503  CACGTG              
BsiEI        2649  CGGCCG               CG[GA][CT]^CG
             3038  CGGCCG              
BsrBI         904  CCGCTC               CCGCTC(-3/-3)
              292  GAGCGG              
DraIII        812  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2500  CACCACGTG           
EagI         2649  CGGCCG               C^GGCCG
             3038  CGGCCG              
EclXI        2649  CGGCCG               C^GGCCG
             3038  CGGCCG              
Eco52I       2649  CGGCCG               C^GGCCG
             3038  CGGCCG              
EcoNI         756  CCTTCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2850  CCTTGCGGAGG         
HphI          276  GGTGA                GGTGA(8/7)
             3078  GGTGA               
NspI           77  GCATGC               [GA]CATG^[CT]
             1574  ACATGC              
SmlI         1375  CTCAAG               C^T[CT][GA]AG
             2604  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        KasI        Ksp632I     MaeII       MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        StuI        StyI        TaqI        TseI        
TspRI       TthHB8I     XcmI        XmaCI       XmaI        
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