################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BclI BfrI BglII BlnI BsaBI BsiWI BsmBI BsmI BspDI BspLU11I BsrDI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI MamI MfeI MluI MseI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tru9I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1705 GTCTAC GT^[AC][GT]AC AccIII 2829 TCCGGA T^CCGGA Alw44I 1876 GTGCAC G^TGCAC Aor51HI 2778 AGCGCT AGC^GCT ApaLI 1876 GTGCAC G^TGCAC AscI 1101 GGCGCGCC GG^CGCGCC AspI 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 2503 CACGTG CAC^GTG BbsI 2914 GTCTTC GAAGAC(2/6) BbvCI 474 GCTGAGG CCTCAGC(-5/-2) BcgI 561 GCAGCGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3068 GTATCC GTATCC(6/5) BfaI 1426 CTAG C^TAG BlpI 93 GCTGAGC GC^T[ACGT]AGC Bpu1102I 93 GCTGAGC GC^T[ACGT]AGC BpuAI 2914 GTCTTC GAAGAC(2/6) BsaI 649 GAGACC GGTCTC(1/5) BsaWI 2829 TCCGGA [AT]^CCGG[AT] BseAI 2829 TCCGGA T^CCGGA Bsp1407I 1727 TGTACA T^GTACA BspEI 2829 TCCGGA T^CCGGA BspHI 1256 TCATGA T^CATGA BsrGI 1727 TGTACA T^GTACA BssHII 1102 GCGCGC G^CGCGC BstYI 3194 AGATCC [GA]^GATC[CT] CelII 93 GCTGAGC GC^T[ACGT]AGC Eco47III 2778 AGCGCT AGC^GCT FseI 318 GGCCGGCC GGCCGG^CC HincII 973 GTCAAC GT[CT]^[GA]AC HindII 973 GTCAAC GT[CT]^[GA]AC KspI 926 CCGCGG CCGC^GG MaeI 1426 CTAG C^TAG MaeIII 338 GTGAC ^GT[ACGT]AC MflI 3194 AGATCC [GA]^GATC[CT] MroI 2829 TCCGGA T^CCGGA NruI 3164 TCGCGA TCG^CGA PflFI 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2503 CACGTG CAC^GTG PmlI 2503 CACGTG CAC^GTG RcaI 1256 TCATGA T^CATGA SacII 926 CCGCGG CCGC^GG SphI 77 GCATGC GCATG^C SspBI 1727 TGTACA T^GTACA TfiI 1618 GAATC G^A[AT]TC Tsp45I 338 GTGAC ^GT[GC]AC Tsp509I 434 AATT ^AATT Tth111I 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 3194 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsaAI 328 TACGTG [CT]AC^GT[GA] 2503 CACGTG BsiEI 2649 CGGCCG CG[GA][CT]^CG 3038 CGGCCG BsrBI 904 CCGCTC CCGCTC(-3/-3) 292 GAGCGG DraIII 812 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 2500 CACCACGTG EagI 2649 CGGCCG C^GGCCG 3038 CGGCCG EclXI 2649 CGGCCG C^GGCCG 3038 CGGCCG Eco52I 2649 CGGCCG C^GGCCG 3038 CGGCCG EcoNI 756 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2850 CCTTGCGGAGG HphI 276 GGTGA GGTGA(8/7) 3078 GGTGA NspI 77 GCATGC [GA]CATG^[CT] 1574 ACATGC SmlI 1375 CTCAAG C^T[CT][GA]AG 2604 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I MaeII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StuI StyI TaqI TseI TspRI TthHB8I XcmI XmaCI XmaI