Restriction map for
pF1KA2027
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp700I Asp718I AspI AviII
AvrII BalI BbeI BbrPI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2047 GACGTC GACGT^C
AccIII 55 TCCGGA T^CCGGA
AcsI 1459 AAATTT [GA]^AATT[CT]
AflIII 1297 ACGTGT A^C[GA][CT]GT
AhdI 2101 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1174 GTGCAC G^TGCAC
AocI 1627 CCTCAGG CC^T[ACGT]AGG
ApaI 765 GGGCCC GGGCC^C
ApaLI 1174 GTGCAC G^TGCAC
ApoI 1459 AAATTT [GA]^AATT[CT]
AseI 2166 ATTAAT AT^TAAT
AsnI 2166 ATTAAT AT^TAAT
AspEI 2101 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1581 GGATCC G^GATCC
BbvCI 165 CCTCAGC CCTCAGC(-5/-2)
BciVI 1013 GTATCC GTATCC(6/5)
BglI 767 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1296 TACGTG [CT]AC^GT[GA]
BsaBI 1410 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 55 TCCGGA [AT]^CCGG[AT]
BseAI 55 TCCGGA T^CCGGA
BseRI 1625 CTCCTC GAGGAG(10/8)
BsmBI 996 CGTCTC CGTCTC(1/5)
BsmI 126 GAATGC GAATGC(1/-1)
BspEI 55 TCCGGA T^CCGGA
BstAPI 1951 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1627 CCTCAGG CC^T[ACGT]AGG
CfoI 2003 GCGC GCG^C
DsaI 1832 CCATGG C^C[GA][CT]GG
EaeI 1503 CGGCCA [CT]^GGCC[GA]
Eam1105I 2101 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1342 GAAGAG CTCTTC(1/4)
Eco81I 1627 CCTCAGG CC^T[ACGT]AGG
HhaI 2003 GCGC GCG^C
HinP1I 2003 GCGC G^CGC
HincII 1347 GTCAAC GT[CT]^[GA]AC
HindII 1347 GTCAAC GT[CT]^[GA]AC
Ksp632I 1342 GAAGAG CTCTTC(1/4)
MamI 1410 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 55 TCCGGA T^CCGGA
MslI 1087 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1832 CCATGG C^CATGG
NheI 1128 GCTAGC G^CTAGC
NruI 2120 TCGCGA TCG^CGA
PshBI 2166 ATTAAT AT^TAAT
SacI 1111 GAGCTC GAGCT^C
SexAI 1239 ACCAGGT A^CC[AT]GGT
SmlI 1924 CTCAAG C^T[CT][GA]AG
SphI 1423 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1200 GTCTAC GT^[AC][GT]AC
1650 GTCTAC
AccII 1909 CGCG CG^CG
2121 CGCG
AvaI 336 CCCGAG C^[CT]CG[GA]G
762 CTCGGG
BanI 145 GGTGCC G^G[CT][GA]CC
849 GGTGCC
BanII 765 GGGCCC G[GA]GC[CT]^C
1111 GAGCTC
BclI 420 TGATCA T^GATCA
1563 TGATCA
BcnI 289 CCCGG CC^[GC]GG
2192 CCCGG
BsaI 214 GAGACC GGTCTC(1/5)
1275 GGTCTC
BsoBI 336 CCCGAG C^[CT]CG[GA]G
762 CTCGGG
BstUI 1909 CGCG CG^CG
2121 CGCG
EcoNI 192 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
517 CCTGTGGGAGG
FauI 322 CCCGC CCCGC(4/6)
385 CCCGC
FbaI 420 TGATCA T^GATCA
1563 TGATCA
MaeII 1297 ACGT A^CGT
2048 ACGT
MvnI 1909 CGCG CG^CG
2121 CGCG
NciI 289 CCCGG CC^[GC]GG
2192 CCCGG
NspI 826 GCATGT [GA]CATG^[CT]
1423 GCATGC
PflMI 1101 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1849 CCAGGTTCTGG
PpuMI 697 AGGTCCT [GA]G^G[AT]CC[CT]
1247 GGGTCCT
TaqI 105 TCGA T^CGA
983 TCGA
TthHB8I 105 TCGA T^CGA
983 TCGA
Van91I 1101 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1849 CCAGGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII