Restriction map for
pF1KB3158
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AviII BamHI BbeI
BbrPI BbsI BcgI BciVI BfrI
BlpI Bpu1102I BpuAI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HaeII KasI
KpnI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SapI SbfI SexAI
SfiI SfoI SfuI SmlI SnaBI
SpeI SphI Sse8387I SwaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 150 CGCG CG^CG
AcyI 5 GGCGTC G[GA]^CG[CT]C
AgeI 244 ACCGGT A^CCGGT
AhdI 217 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1291 GATCC GGATC(4/5)
AlwNI 1910 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 475 CCTCAGG CC^T[ACGT]AGG
Asp700I 1867 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 217 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 157 GTGCTC G[AT]GC[AT]^C
AvrII 2287 CCTAGG C^CTAGG
BanI 925 GGCACC G^G[CT][GA]CC
BanII 2365 GGGCTC G[GA]GC[CT]^C
BclI 2394 TGATCA T^GATCA
BglI 1786 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 557 AGATCT A^GATCT
BlnI 2287 CCTAGG C^CTAGG
BsaAI 307 TACGTG [CT]AC^GT[GA]
BsaBI 507 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5 GGCGTC G[GA]^CG[CT]C
BsaWI 244 ACCGGT [AT]^CCGG[AT]
BsgI 337 CTGCAC GTGCAG(16/14)
BsiHKAI 157 GTGCTC G[AT]GC[AT]^C
Bsp1407I 125 TGTACA T^GTACA
BsrFI 244 ACCGGT [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
BstUI 150 CGCG CG^CG
Bsu36I 475 CCTCAGG CC^T[ACGT]AGG
Cac8I 2076 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 244 ACCGGT [GA]^CCGG[CT]
Eam1105I 217 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 475 CCTCAGG CC^T[ACGT]AGG
EcoRI 1685 GAATTC G^AATTC
EcoRV 2119 GATATC GAT^ATC
FbaI 2394 TGATCA T^GATCA
Hin1I 5 GGCGTC G[GA]^CG[CT]C
HindIII 2607 AAGCTT A^AGCTT
KspI 149 CCGCGG CCGC^GG
MamI 507 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 150 CGCG CG^CG
NcoI 675 CCATGG C^CATGG
NdeI 2734 CATATG CA^TATG
PinAI 244 ACCGGT A^CCGGT
SacII 149 CCGCGG CCGC^GG
SalI 2227 GTCGAC G^TCGAC
ScaI 2178 AGTACT AGT^ACT
SgrAI 243 CACCGGTG C[GA]^CCGG[CT]G
SmaI 287 CCCGGG CCC^GGG
SspBI 125 TGTACA T^GTACA
StuI 2290 AGGCCT AGG^CCT
XbaI 2115 TCTAGA T^CTAGA
XcmI 1391 CCACAAAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 287 CCCGGG C^CCGGG
XmaI 287 CCCGGG C^CCGGG
XmnI 1867 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 8 GTCTAC GT^[AC][GT]AC
2227 GTCGAC
AcsI 1685 GAATTC [GA]^AATT[CT]
2691 AAATTT
ApoI 1685 GAATTC [GA]^AATT[CT]
2691 AAATTT
AspI 1672 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2452 GACACTGTC
BalI 300 TGGCCA TGG^CCA
602 TGGCCA
BbvCI 793 GCTGAGG CCTCAGC(-5/-2)
1998 GCTGAGG
BpmI 1049 CTGGAG CTGGAG(16/14)
1090 CTGGAG
BsaI 1387 GAGACC GGTCTC(1/5)
806 GGTCTC
BsmI 912 GAATGC GAATGC(1/-1)
2247 GAATGC
BspMI 44 ACCTGC ACCTGC(4/8)
182 ACCTGC
BsrBI 324 CCGCTC CCGCTC(-3/-3)
405 GAGCGG
BsrDI 1269 CATTGC GCAATG(2/0)
2345 CATTGC
BstXI 2423 CCACAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2618 CCATCACACTGG
BstYI 557 AGATCT [GA]^GATC[CT]
1290 AGATCC
CfoI 240 GCGC GCG^C
720 GCGC
DsaI 149 CCGCGG C^C[GA][CT]GG
675 CCATGG
EcoT14I 675 CCATGG C^C[AT][AT]GG
2287 CCTAGG
HgaI 1918 GACGC GACGC(5/10)
6 GCGTC
HhaI 240 GCGC GCG^C
720 GCGC
HinP1I 240 GCGC G^CGC
720 GCGC
HpaI 1258 GTTAAC GTT^AAC
1312 GTTAAC
MaeII 308 ACGT A^CGT
695 ACGT
MfeI 864 CAATTG C^AATTG
932 CAATTG
MflI 557 AGATCT [GA]^GATC[CT]
1290 AGATCC
MluNI 300 TGGCCA TGG^CCA
602 TGGCCA
MscI 300 TGGCCA TGG^CCA
602 TGGCCA
MunI 864 CAATTG C^AATTG
932 CAATTG
PflFI 1672 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2452 GACACTGTC
PflMI 945 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1876 CCAGGCTATGG
PshAI 439 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1819 GACAACAGTC
PstI 203 CTGCAG CTGCA^G
2350 CTGCAG
SspI 1376 AATATT AAT^ATT
2767 AATATT
StyI 675 CCATGG C^C[AT][AT]GG
2287 CCTAGG
Tth111I 1672 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2452 GACACTGTC
Van91I 945 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1876 CCAGGCTATGG
XhoII 557 AGATCT [GA]^GATC[CT]
1290 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AseI AsnI
AvaI AvaII BbvI BcnI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I