Restriction map for
pF1KB3463
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AclI Alw44I
Aor51HI ApaI ApaLI AscI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BcnI BlnI BlpI Bpu1102I
BsaI BseAI BsiEI BsiWI Bsp1407I
BspEI BspLU11I BsrGI BssHII Bst1107I
BstZ17I CelII CpoI EagI EclXI
Eco47III Eco52I FseI FspI HindIII
KasI KspI MluI MroI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PaeR7I PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3415 GGTACC G^GTACC
AccII 1612 CGCG CG^CG
AcyI 3690 GGCGTC G[GA]^CG[CT]C
AflII 1407 CTTAAG C^TTAAG
AgeI 2359 ACCGGT A^CCGGT
AocI 2404 CCTGAGG CC^T[ACGT]AGG
AseI 141 ATTAAT AT^TAAT
AsnI 141 ATTAAT AT^TAAT
Asp718I 3415 GGTACC G^GTACC
AspI 852 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1305 GGATCC G^GATCC
BanII 4225 GAGCCC G[GA]GC[CT]^C
BfrI 1407 CTTAAG C^TTAAG
BsaAI 4323 CACGTA [CT]AC^GT[GA]
BsaBI 616 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3690 GGCGTC G[GA]^CG[CT]C
BsmBI 1267 CGTCTC CGTCTC(1/5)
BsmI 357 GAATGC GAATGC(1/-1)
BspDI 2653 ATCGAT AT^CGAT
BspHI 2343 TCATGA T^CATGA
BspMI 777 ACCTGC ACCTGC(4/8)
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BsrFI 2359 ACCGGT [GA]^CCGG[CT]
BssSI 4672 CTCGTG CACGAG(-5/-1)
BstBI 3152 TTCGAA TT^CGAA
BstEII 1471 GGTGACC G^GT[ACGT]ACC
BstPI 1471 GGTGACC G^GT[ACGT]ACC
BstUI 1612 CGCG CG^CG
Bsu36I 2404 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2359 ACCGGT [GA]^CCGG[CT]
ClaI 2653 ATCGAT AT^CGAT
DraIII 1240 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 549 GACTCATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2404 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1471 GGTGACC G^GT[ACGT]ACC
EcoRI 2462 GAATTC G^AATTC
FauI 1610 CCCGC CCCGC(4/6)
Hin1I 3690 GGCGTC G[GA]^CG[CT]C
HpaI 478 GTTAAC GTT^AAC
KpnI 3415 GGTACC GGTAC^C
MamI 616 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1612 CGCG CG^CG
NcoI 260 CCATGG C^CATGG
NdeI 3410 CATATG CA^TATG
NspV 3152 TTCGAA TT^CGAA
PflFI 852 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2359 ACCGGT A^CCGGT
PshBI 141 ATTAAT AT^TAAT
RcaI 2343 TCATGA T^CATGA
SexAI 1686 ACCTGGT A^CC[AT]GGT
SfuI 3152 TTCGAA TT^CGAA
SpeI 2541 ACTAGT A^CTAGT
StuI 2088 AGGCCT AGG^CCT
Tth111I 852 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 661 ACACGT A^C[GA][CT]GT
4322 ACACGT
AhdI 3775 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3844 GACTCCATGTC
AlwNI 3886 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3896 CAGTCCCTG
AspEI 3775 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3844 GACTCCATGTC
AvaI 52 CCCGAG C^[CT]CG[GA]G
64 CCCGAG
BalI 2591 TGGCCA TGG^CCA
3492 TGGCCA
BanI 3415 GGTACC G^G[CT][GA]CC
3549 GGTGCC
BglI 3313 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4707 GCCTCAGAGGC
BglII 747 AGATCT A^GATCT
2943 AGATCT
BsgI 3602 CTGCAC GTGCAG(16/14)
4606 GTGCAG
BsoBI 52 CCCGAG C^[CT]CG[GA]G
64 CCCGAG
BstAPI 1056 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4716 GCATAATTTGC
CfoI 23 GCGC GCG^C
450 GCGC
DraI 963 TTTAAA TTT^AAA
1137 TTTAAA
DsaI 11 CCGTGG C^C[GA][CT]GG
260 CCATGG
EaeI 2591 TGGCCA [CT]^GGCC[GA]
3492 TGGCCA
Eam1105I 3775 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3844 GACTCCATGTC
EcoNI 3736 CCTCACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4738 CCTAAACTAGG
EcoRV 616 GATATC GAT^ATC
2239 GATATC
HaeII 22 GGCGCT [GA]GCGC^[CT]
449 AGCGCC
HgaI 1266 GCGTC GACGC(5/10)
3691 GCGTC
HhaI 23 GCGC GCG^C
450 GCGC
HinP1I 23 GCGC G^CGC
450 GCGC
MfeI 808 CAATTG C^AATTG
3831 CAATTG
MluNI 2591 TGGCCA TGG^CCA
3492 TGGCCA
MscI 2591 TGGCCA TGG^CCA
3492 TGGCCA
MunI 808 CAATTG C^AATTG
3831 CAATTG
PflMI 212 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4333 CCAAACTATGG
PpuMI 675 AGGACCT [GA]G^G[AT]CC[CT]
4563 AGGACCC
PshAI 3194 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4075 GACAGTGGTC
Van91I 212 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4333 CCAAACTATGG
XcmI 2207 CCACCAGTATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3908 CCACTGGGGCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI