Restriction map for
pF1KB3472
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BglI BlnI BlpI BpmI Bpu1102I
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoT14I
EcoT22I FseI FspI Hin1I HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1870 GTAGAC GT^[AC][GT]AC
AflII 997 CTTAAG C^TTAAG
Aor51HI 1877 AGCGCT AGC^GCT
Asp700I 705 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1926 GTGCTC G[AT]GC[AT]^C
AvaI 2259 CCCGAG C^[CT]CG[GA]G
BalI 2120 TGGCCA TGG^CCA
BanI 1858 GGTGCC G^G[CT][GA]CC
BanII 298 GAGCCC G[GA]GC[CT]^C
BbrPI 1493 CACGTG CAC^GTG
BfrI 997 CTTAAG C^TTAAG
BglII 984 AGATCT A^GATCT
BsaAI 1493 CACGTG [CT]AC^GT[GA]
BsaBI 924 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 474 ACCGGA [AT]^CCGG[AT]
BsgI 1490 CTGCAC GTGCAG(16/14)
BsiHKAI 1926 GTGCTC G[AT]GC[AT]^C
BsoBI 2259 CCCGAG C^[CT]CG[GA]G
BspDI 1568 ATCGAT AT^CGAT
Cac8I 2105 GCTGGC GC[ACGT]^[ACGT]GC
ClaI 1568 ATCGAT AT^CGAT
DsaI 2149 CCACGG C^C[GA][CT]GG
EaeI 2120 TGGCCA [CT]^GGCC[GA]
Eco47III 1877 AGCGCT AGC^GCT
EcoRV 928 GATATC GAT^ATC
HincII 199 GTTGAC GT[CT]^[GA]AC
HindII 199 GTTGAC GT[CT]^[GA]AC
HindIII 1718 AAGCTT A^AGCTT
MamI 924 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1931 CAATTG C^AATTG
MluNI 2120 TGGCCA TGG^CCA
MscI 2120 TGGCCA TGG^CCA
MslI 42 CACCGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1931 CAATTG C^AATTG
NdeI 1178 CATATG CA^TATG
NspI 1002 GCATGT [GA]CATG^[CT]
PflMI 1026 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1493 CACGTG CAC^GTG
PmlI 1493 CACGTG CAC^GTG
SexAI 945 ACCAGGT A^CC[AT]GGT
SmlI 997 CTTAAG C^T[CT][GA]AG
SspI 1945 AATATT AAT^ATT
Van91I 1026 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 705 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 207 GAATTC [GA]^AATT[CT]
1368 GAATTC
AlwI 1571 GATCC GGATC(4/5)
2300 GATCC
AocI 781 CCTCAGG CC^T[ACGT]AGG
1510 CCTGAGG
ApoI 207 GAATTC [GA]^AATT[CT]
1368 GAATTC
BbsI 705 GAAGAC GAAGAC(2/6)
1096 GAAGAC
BclI 1050 TGATCA T^GATCA
1886 TGATCA
BcnI 4 CCGGG CC^[GC]GG
1574 CCGGG
BfaI 428 CTAG C^TAG
982 CTAG
BpuAI 705 GAAGAC GAAGAC(2/6)
1096 GAAGAC
BsmAI 61 GAGAC GTCTC(1/5)
1406 GAGAC
BsrDI 1112 CATTGC GCAATG(2/0)
46 GCAATG
BstYI 984 AGATCT [GA]^GATC[CT]
2299 AGATCC
Bsu36I 781 CCTCAGG CC^T[ACGT]AGG
1510 CCTGAGG
CfoI 557 GCGC GCG^C
1878 GCGC
Eco81I 781 CCTCAGG CC^T[ACGT]AGG
1510 CCTGAGG
EcoRI 207 GAATTC G^AATTC
1368 GAATTC
FbaI 1050 TGATCA T^GATCA
1886 TGATCA
HaeII 556 GGCGCT [GA]GCGC^[CT]
1877 AGCGCT
HhaI 557 GCGC GCG^C
1878 GCGC
HinP1I 557 GCGC G^CGC
1878 GCGC
HphI 989 TCACC GGTGA(8/7)
1433 TCACC
MaeI 428 CTAG C^TAG
982 CTAG
MaeII 1494 ACGT A^CGT
1864 ACGT
MflI 984 AGATCT [GA]^GATC[CT]
2299 AGATCC
NciI 4 CCGGG CC^[GC]GG
1574 CCGGG
PvuII 1790 CAGCTG CAG^CTG
1978 CAGCTG
TaqI 1569 TCGA T^CGA
2193 TCGA
TthHB8I 1569 TCGA T^CGA
2193 TCGA
XcmI 963 CCACATACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1736 CCATCAGGCTTTTGG
XhoII 984 AGATCT [GA]^GATC[CT]
2299 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI