Restriction map for
pF1KB3647
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
AocI ApaI AscI AseI AsnI
AspEI AvrII BbeI BcgI BclI
BfrI BglII BlnI BsiWI BspDI
BspLU11I BsrDI BstAPI BstBI BstEII
BstPI Bsu36I ClaI DrdI Eam1105I
Eco81I EcoO65I EcoT22I FbaI FseI
HindIII HpaI KasI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI SacII SalI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 74 enzymes) #
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AatII 1426 GACGTC GACGT^C
Acc65I 1343 GGTACC G^GTACC
AccI 1045 GTATAC GT^[AC][GT]AC
AccIII 402 TCCGGA T^CCGGA
Alw44I 514 GTGCAC G^TGCAC
AlwNI 1609 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1649 AGCGCT AGC^GCT
ApaLI 514 GTGCAC G^TGCAC
Asp718I 1343 GGTACC G^GTACC
AspI 1624 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 634 CCCGGG C^[CT]CG[GA]G
AviII 98 TGCGCA TGC^GCA
BamHI 1029 GGATCC G^GATCC
BbrPI 742 CACGTG CAC^GTG
BbsI 1022 GTCTTC GAAGAC(2/6)
BciVI 1473 GTATCC GTATCC(6/5)
BfaI 607 CTAG C^TAG
BlpI 766 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 766 GCTGAGC GC^T[ACGT]AGC
BpuAI 1022 GTCTTC GAAGAC(2/6)
BsaAI 742 CACGTG [CT]AC^GT[GA]
BsaBI 459 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 941 GAGACC GGTCTC(1/5)
BseAI 402 TCCGGA T^CCGGA
BsiEI 127 CGGCCG CG[GA][CT]^CG
BsmBI 107 GAGACG CGTCTC(1/5)
BsmI 1188 GAATGC GAATGC(1/-1)
BsoBI 634 CCCGGG C^[CT]CG[GA]G
BspEI 402 TCCGGA T^CCGGA
BspHI 1679 TCATGA T^CATGA
BsrBI 1138 GAGCGG CCGCTC(-3/-3)
BssSI 118 CACGAG CACGAG(-5/-1)
Bst1107I 1045 GTATAC GTA^TAC
BstXI 919 CCAAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1045 GTATAC GTA^TAC
CelII 766 GCTGAGC GC^T[ACGT]AGC
CpoI 508 CGGTCCG CG^G[AT]CCG
DraI 1320 TTTAAA TTT^AAA
DraIII 417 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 127 CGGCCG C^GGCCG
EclXI 127 CGGCCG C^GGCCG
Eco47III 1649 AGCGCT AGC^GCT
Eco52I 127 CGGCCG C^GGCCG
EcoNI 546 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 748 GAATTC G^AATTC
EcoRV 459 GATATC GAT^ATC
FspI 98 TGCGCA TGC^GCA
HincII 994 GTCAAC GT[CT]^[GA]AC
HindII 994 GTCAAC GT[CT]^[GA]AC
KpnI 1343 GGTACC GGTAC^C
MaeI 607 CTAG C^TAG
MaeIII 721 GTCAC ^GT[ACGT]AC
MamI 459 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 402 TCCGGA T^CCGGA
NcoI 1482 CCATGG C^CATGG
NspI 902 GCATGT [GA]CATG^[CT]
PflFI 1624 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1608 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 742 CACGTG CAC^GTG
PmlI 742 CACGTG CAC^GTG
RcaI 1679 TCATGA T^CATGA
RsrII 508 CGGTCCG CG^G[AT]CCG
SacI 430 GAGCTC GAGCT^C
ScaI 437 AGTACT AGT^ACT
SmaI 634 CCCGGG CCC^GGG
SmlI 433 CTCAAG C^T[CT][GA]AG
StuI 672 AGGCCT AGG^CCT
TaqI 1736 TCGA T^CGA
Tsp45I 721 GTCAC ^GT[GC]AC
Tth111I 1624 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1736 TCGA T^CGA
Van91I 1608 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 634 CCCGGG C^CCGGG
XmaI 634 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 556 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1002 GAAGTTCTTC
BalI 74 TGGCCA TGG^CCA
143 TGGCCA
BanI 1343 GGTACC G^G[CT][GA]CC
1537 GGTGCC
BanII 256 GGGCTC G[GA]GC[CT]^C
430 GAGCTC
BbvCI 150 CCTCAGC CCTCAGC(-5/-2)
1204 GCTGAGG
BglI 136 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1724 GCCTGGCTGGC
BsaWI 402 TCCGGA [AT]^CCGG[AT]
797 ACCGGA
BssHII 132 GCGCGC G^CGCGC
155 GCGCGC
BstYI 122 AGATCC [GA]^GATC[CT]
1029 GGATCC
DsaI 876 CCACGG C^C[GA][CT]GG
1482 CCATGG
MaeII 743 ACGT A^CGT
1427 ACGT
MflI 122 AGATCC [GA]^GATC[CT]
1029 GGATCC
MluNI 74 TGGCCA TGG^CCA
143 TGGCCA
MscI 74 TGGCCA TGG^CCA
143 TGGCCA
NaeI 55 GCCGGC GCC^GGC
83 GCCGGC
NgoMIV 55 GCCGGC G^CCGGC
83 GCCGGC
PleI 17 GAGTC GAGTC(4/5)
1596 GAGTC
SspI 1060 AATATT AAT^ATT
1467 AATATT
TfiI 1073 GAATC G^A[AT]TC
1266 GAATC
XcmI 342 CCATGAGAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
919 CCAAAAGACTGGTGG
XhoII 122 AGATCC [GA]^GATC[CT]
1029 GGATCC
XmnI 556 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1002 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SspBI StyI Tru9I
TseI Tsp509I TspRI