Restriction map for
pF1KB3701
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# NO CUT ENZYMES ( 79 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
HindIII HpaI KpnI MaeI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1837 GACGTC GACGT^C
AccIII 1190 TCCGGA T^CCGGA
Asp700I 2881 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1027 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1745 TGCGCA TGC^GCA
BbrPI 1048 CACGTG CAC^GTG
BbvCI 1054 GCTGAGG CCTCAGC(-5/-2)
BcgI 3042 CGATGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 743 AGATCT A^GATCT
BsaAI 1048 CACGTG [CT]AC^GT[GA]
BseAI 1190 TCCGGA T^CCGGA
BspEI 1190 TCCGGA T^CCGGA
BssHII 1150 GCGCGC G^CGCGC
FseI 3210 GGCCGGCC GGCCGG^CC
FspI 1745 TGCGCA TGC^GCA
HincII 973 GTCAAC GT[CT]^[GA]AC
HindII 973 GTCAAC GT[CT]^[GA]AC
KspI 261 CCGCGG CCGC^GG
MroI 1190 TCCGGA T^CCGGA
NaeI 3211 GCCGGC GCC^GGC
NgoMIV 3211 GCCGGC G^CCGGC
NspI 2054 ACATGC [GA]CATG^[CT]
PaeR7I 1661 CTCGAG C^TCGAG
PflFI 1027 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1048 CACGTG CAC^GTG
PmlI 1048 CACGTG CAC^GTG
SacII 261 CCGCGG CCGC^GG
SexAI 1028 ACCTGGT A^CC[AT]GGT
SmlI 1661 CTCGAG C^T[CT][GA]AG
Tsp509I 646 AATT ^AATT
Tth111I 1027 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1661 CTCGAG C^TCGAG
XmnI 2881 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 1024 GTAGAC GT^[AC][GT]AC
2983 GTAGAC
AflIII 2321 ACACGT A^C[GA][CT]GT
2579 ACGTGT
Alw44I 340 GTGCAC G^TGCAC
619 GTGCAC
AocI 30 CCTGAGG CC^T[ACGT]AGG
1648 CCTGAGG
ApaLI 340 GTGCAC G^TGCAC
619 GTGCAC
BbeI 89 GGCGCC GGCGC^C
3339 GGCGCC
BsaBI 141 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3360 GATAGACATC
BsaWI 407 TCCGGT [AT]^CCGG[AT]
1190 TCCGGA
BsrDI 477 CATTGC GCAATG(2/0)
1287 CATTGC
BssSI 541 CACGAG CACGAG(-5/-1)
1739 CTCGTG
BstAPI 1386 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2077 GCAGGAAGTGC
BstEII 1131 GGTCACC G^GT[ACGT]ACC
3444 GGTGACC
BstPI 1131 GGTCACC G^GT[ACGT]ACC
3444 GGTGACC
Bsu36I 30 CCTGAGG CC^T[ACGT]AGG
1648 CCTGAGG
DrdI 1576 GACCCCGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1966 GACAACGTGGTC
Eco81I 30 CCTGAGG CC^T[ACGT]AGG
1648 CCTGAGG
EcoNI 528 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1083 CCTGCGGCAGG
EcoO65I 1131 GGTCACC G^GT[ACGT]ACC
3444 GGTGACC
KasI 89 GGCGCC G^GCGCC
3339 GGCGCC
MamI 141 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3360 GATAGACATC
NarI 89 GGCGCC GG^CGCC
3339 GGCGCC
SfiI 2433 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3384 GGCCAAGGTGGCC
SfoI 89 GGCGCC GGC^GCC
3339 GGCGCC
XcmI 2051 CCAACATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2690 CCAACCAGATGCTGG
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I SbfI ScaI
ScrFI SfaNI SfcI SmaI Sse8387I
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI