Restriction map for
pF1KB3947
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspHI AspI AviII AvrII BamHI
BbeI BcgI BciVI BfrI BglI
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 544 GGTACC G^GTACC
AccII 679 CGCG CG^CG
AflIII 104 ACATGT A^C[GA][CT]GT
AhdI 2579 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 810 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 544 GGTACC G^GTACC
AspEI 2579 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1688 TGGCCA TGG^CCA
BanII 2214 GGGCTC G[GA]GC[CT]^C
BbrPI 1790 CACGTG CAC^GTG
BbvCI 2437 CCTCAGC CCTCAGC(-5/-2)
BclI 944 TGATCA T^GATCA
BcnI 2504 CCCGG CC^[GC]GG
BglII 125 AGATCT A^GATCT
BmyI 2214 GGGCTC G[AGT]GC[ACT]^C
BpmI 1525 CTCCAG CTGGAG(16/14)
BsaAI 1790 CACGTG [CT]AC^GT[GA]
BsgI 2382 CTGCAC GTGCAG(16/14)
Bsp1286I 2214 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 104 ACATGT A^CATGT
BspMI 2076 ACCTGC ACCTGC(4/8)
BsrDI 2283 CATTGC GCAATG(2/0)
BstAPI 421 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 17 TTCGAA TT^CGAA
BstUI 679 CGCG CG^CG
BstYI 125 AGATCT [GA]^GATC[CT]
EaeI 1688 TGGCCA [CT]^GGCC[GA]
Eam1105I 2579 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 944 TGATCA T^GATCA
HapII 2505 CCGG C^CGG
HpaII 2505 CCGG C^CGG
KpnI 544 GGTACC GGTAC^C
MfeI 1098 CAATTG C^AATTG
MflI 125 AGATCT [GA]^GATC[CT]
MluNI 1688 TGGCCA TGG^CCA
MscI 1688 TGGCCA TGG^CCA
MspA1I 2379 CAGCTG C[AC]G^C[GT]G
MspI 2505 CCGG C^CGG
MunI 1098 CAATTG C^AATTG
MvnI 679 CGCG CG^CG
NciI 2504 CCCGG CC^[GC]GG
NspV 17 TTCGAA TT^CGAA
PmaCI 1790 CACGTG CAC^GTG
PmlI 1790 CACGTG CAC^GTG
PvuII 2379 CAGCTG CAG^CTG
ScaI 1281 AGTACT AGT^ACT
SfuI 17 TTCGAA TT^CGAA
SmlI 303 CTCAAG C^T[CT][GA]AG
SphI 2476 GCATGC GCATG^C
SspI 466 AATATT AAT^ATT
XhoII 125 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 678 CCGC CCGC(-3/-1)
2273 CCGC
AlwI 826 GATCC GGATC(4/5)
259 GGATC
AvaI 668 CTCGGG C^[CT]CG[GA]G
716 CCCGAG
BanI 544 GGTACC G^G[CT][GA]CC
1732 GGTGCC
BbsI 2655 GAAGAC GAAGAC(2/6)
1784 GTCTTC
BlpI 702 GCTCAGC GC^T[ACGT]AGC
1417 GCTAAGC
Bpu1102I 702 GCTCAGC GC^T[ACGT]AGC
1417 GCTAAGC
BpuAI 2655 GAAGAC GAAGAC(2/6)
1784 GTCTTC
BseRI 833 CTCCTC GAGGAG(10/8)
1257 CTCCTC
BsmI 20 GAATGC GAATGC(1/-1)
1359 GAATGC
BsoBI 668 CTCGGG C^[CT]CG[GA]G
716 CCCGAG
BspHI 623 TCATGA T^CATGA
1461 TCATGA
CelII 702 GCTCAGC GC^T[ACGT]AGC
1417 GCTAAGC
DraII 492 AGGTCCT [GA]G^G[ACGT]CC[CT]
1623 AGGACCC
DsaI 1961 CCATGG C^C[GA][CT]GG
2222 CCATGG
EcoO109I 492 AGGTCCT [GA]G^G[ACGT]CC[CT]
1623 AGGACCC
EcoT22I 22 ATGCAT ATGCA^T
207 ATGCAT
HgaI 1919 GACGC GACGC(5/10)
1782 GCGTC
HincII 251 GTCAAC GT[CT]^[GA]AC
2191 GTCAAC
HindII 251 GTCAAC GT[CT]^[GA]AC
2191 GTCAAC
HindIII 368 AAGCTT A^AGCTT
1504 AAGCTT
NcoI 1961 CCATGG C^CATGG
2222 CCATGG
NsiI 22 ATGCAT ATGCA^T
207 ATGCAT
PpuMI 492 AGGTCCT [GA]G^G[AT]CC[CT]
1623 AGGACCC
PstI 1707 CTGCAG CTGCA^G
1805 CTGCAG
RcaI 623 TCATGA T^CATGA
1461 TCATGA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BssSI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I