Restriction map for
pF1KB4057
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eco47III Eco81I
EcoO65I EcoRI EcoT22I FbaI FspI
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1468 GACGTC GACGT^C
AcsI 540 GAATTT [GA]^AATT[CT]
AhdI 1222 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1234 GTGCAC G^TGCAC
AlwNI 1063 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1234 GTGCAC G^TGCAC
ApoI 540 GAATTT [GA]^AATT[CT]
Asp700I 2028 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1222 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 739 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1564 CCCGAG C^[CT]CG[GA]G
BsaAI 655 TACGTG [CT]AC^GT[GA]
BsaBI 1770 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1298 GAGACG CGTCTC(1/5)
BsoBI 1564 CCCGAG C^[CT]CG[GA]G
BsrDI 950 GCAATG GCAATG(2/0)
BssSI 1699 CTCGTG CACGAG(-5/-1)
BstXI 269 CCACCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1462 GACTTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1222 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1312 GATATC GAT^ATC
FseI 1878 GGCCGGCC GGCCGG^CC
HaeII 785 GGCGCT [GA]GCGC^[CT]
MamI 1770 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1943 TTAA T^TAA
PflFI 739 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 941 AGTACT AGT^ACT
TfiI 1755 GAATC G^A[AT]TC
Tru9I 1943 TTAA T^TAA
Tsp509I 541 AATT ^AATT
Tth111I 739 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 588 TCTAGA T^CTAGA
XmnI 2028 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 811 CGCG CG^CG
886 CGCG
AccIII 1772 TCCGGA T^CCGGA
2036 TCCGGA
AcyI 742 GGCGTC G[GA]^CG[CT]C
1468 GACGTC
ApaI 1561 GGGCCC GGGCC^C
1691 GGGCCC
AspHI 1152 GAGCAC G[AT]GC[AT]^C
1234 GTGCAC
BalI 822 TGGCCA TGG^CCA
1418 TGGCCA
BbsI 1899 GAAGAC GAAGAC(2/6)
835 GTCTTC
BglI 111 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
841 GCCGCCGTGGC
BpuAI 1899 GAAGAC GAAGAC(2/6)
835 GTCTTC
BsaHI 742 GGCGTC G[GA]^CG[CT]C
1468 GACGTC
BsaI 43 GAGACC GGTCTC(1/5)
56 GAGACC
BsaWI 1772 TCCGGA [AT]^CCGG[AT]
2036 TCCGGA
BseAI 1772 TCCGGA T^CCGGA
2036 TCCGGA
BsiEI 68 CGGCCG CG[GA][CT]^CG
621 CGGCCG
BsiHKAI 1152 GAGCAC G[AT]GC[AT]^C
1234 GTGCAC
BspEI 1772 TCCGGA T^CCGGA
2036 TCCGGA
BstUI 811 CGCG CG^CG
886 CGCG
EagI 68 CGGCCG C^GGCCG
621 CGGCCG
EclXI 68 CGGCCG C^GGCCG
621 CGGCCG
Eco52I 68 CGGCCG C^GGCCG
621 CGGCCG
Hin1I 742 GGCGTC G[GA]^CG[CT]C
1468 GACGTC
HincII 595 GTCAAC GT[CT]^[GA]AC
682 GTCAAC
HindII 595 GTCAAC GT[CT]^[GA]AC
682 GTCAAC
MluNI 822 TGGCCA TGG^CCA
1418 TGGCCA
MroI 1772 TCCGGA T^CCGGA
2036 TCCGGA
MscI 822 TGGCCA TGG^CCA
1418 TGGCCA
MspA1I 431 CAGCGG C[AC]G^C[GT]G
1496 CCGCTG
MvnI 811 CGCG CG^CG
886 CGCG
NaeI 619 GCCGGC GCC^GGC
1879 GCCGGC
NgoMIV 619 GCCGGC G^CCGGC
1879 GCCGGC
PflMI 752 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1983 CCACAAGCTGG
PpuMI 1342 GGGTCCT [GA]G^G[AT]CC[CT]
1739 GGGACCT
StuI 359 AGGCCT AGG^CCT
1361 AGGCCT
TaqI 938 TCGA T^CGA
2003 TCGA
TthHB8I 938 TCGA T^CGA
2003 TCGA
Van91I 752 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1983 CCACAAGCTGG
XcmI 383 CCAGCTTTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1215 CCAGCAGGACGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AvaII BamHI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI XhoII