Restriction map for
pF1KB4264
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BcnI BlnI BpuAI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MspA1I MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 1122 TCCGGA T^CCGGA
AlwI 760 GGATC GGATC(4/5)
AlwNI 1427 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 853 CCTAAGG CC^T[ACGT]AGG
ApaI 1328 GGGCCC GGGCC^C
AseI 1525 ATTAAT AT^TAAT
AsnI 1525 ATTAAT AT^TAAT
BanII 1328 GGGCCC G[GA]GC[CT]^C
BbvCI 2085 GCTGAGG CCTCAGC(-5/-2)
BciVI 259 GTATCC GTATCC(6/5)
BglI 1379 GCCCAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1716 AGATCT A^GATCT
BlpI 142 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 142 GCTAAGC GC^T[ACGT]AGC
BsaI 472 GAGACC GGTCTC(1/5)
BsaWI 1122 TCCGGA [AT]^CCGG[AT]
BseAI 1122 TCCGGA T^CCGGA
BseRI 1340 CTCCTC GAGGAG(10/8)
BsmI 782 GCATTC GAATGC(1/-1)
BspEI 1122 TCCGGA T^CCGGA
BspHI 1358 TCATGA T^CATGA
BspMI 505 ACCTGC ACCTGC(4/8)
BsrDI 456 CATTGC GCAATG(2/0)
Bst1107I 828 GTATAC GTA^TAC
BstZ17I 828 GTATAC GTA^TAC
Bsu36I 853 CCTAAGG CC^T[ACGT]AGG
Cac8I 737 GCATGC GC[ACGT]^[ACGT]GC
CelII 142 GCTAAGC GC^T[ACGT]AGC
Eco81I 853 CCTAAGG CC^T[ACGT]AGG
EcoRI 1672 GAATTC G^AATTC
EcoT22I 868 ATGCAT ATGCA^T
FauI 1332 CCCGC CCCGC(4/6)
HapII 1123 CCGG C^CGG
HincII 1866 GTTGAC GT[CT]^[GA]AC
HindII 1866 GTTGAC GT[CT]^[GA]AC
HpaII 1123 CCGG C^CGG
MfeI 1382 CAATTG C^AATTG
MroI 1122 TCCGGA T^CCGGA
MspI 1123 CCGG C^CGG
MunI 1382 CAATTG C^AATTG
NsiI 868 ATGCAT ATGCA^T
NspI 737 GCATGC [GA]CATG^[CT]
PshBI 1525 ATTAAT AT^TAAT
PstI 1011 CTGCAG CTGCA^G
RcaI 1358 TCATGA T^CATGA
SfcI 1011 CTGCAG C^T[GA][CT]AG
SpeI 832 ACTAGT A^CTAGT
SphI 737 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 794 GTAGAC GT^[AC][GT]AC
828 GTATAC
AflII 7 CTTAAG C^TTAAG
634 CTTAAG
AspHI 19 GTGCTC G[AT]GC[AT]^C
1466 GAGCAC
BalI 815 TGGCCA TGG^CCA
1386 TGGCCA
BclI 1821 TGATCA T^GATCA
1835 TGATCA
BfrI 7 CTTAAG C^TTAAG
634 CTTAAG
BpmI 221 CTGGAG CTGGAG(16/14)
1322 CTGGAG
BsgI 428 CTGCAC GTGCAG(16/14)
1769 CTGCAC
BsiHKAI 19 GTGCTC G[AT]GC[AT]^C
1466 GAGCAC
BsmAI 472 GAGAC GTCTC(1/5)
1082 GAGAC
BstYI 760 GGATCT [GA]^GATC[CT]
1716 AGATCT
DraI 1273 TTTAAA TTT^AAA
1591 TTTAAA
EarI 751 GAAGAG CTCTTC(1/4)
1600 GAAGAG
FbaI 1821 TGATCA T^GATCA
1835 TGATCA
Ksp632I 751 GAAGAG CTCTTC(1/4)
1600 GAAGAG
MflI 760 GGATCT [GA]^GATC[CT]
1716 AGATCT
MluNI 815 TGGCCA TGG^CCA
1386 TGGCCA
MscI 815 TGGCCA TGG^CCA
1386 TGGCCA
PleI 196 GACTC GAGTC(4/5)
422 GACTC
SmlI 7 CTTAAG C^T[CT][GA]AG
634 CTTAAG
Tsp45I 184 GTCAC ^GT[GC]AC
532 GTGAC
XhoII 760 GGATCT [GA]^GATC[CT]
1716 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BfaI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I