Restriction map for
pF1KB4741
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccI AccIII AflII AgeI
AhdI AlwNI ApaI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRV FseI FspI HapII HindIII
HpaI HpaII KasI KpnI MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 3184 GACGTC GACGT^C
AccII 1499 CGCG CG^CG
AclI 1064 AACGTT AA^CGTT
AcyI 3184 GACGTC G[GA]^CG[CT]C
AflIII 196 ACATGT A^C[GA][CT]GT
Alw44I 2068 GTGCAC G^TGCAC
AocI 1990 CCTGAGG CC^T[ACGT]AGG
Aor51HI 148 AGCGCT AGC^GCT
ApaLI 2068 GTGCAC G^TGCAC
AvaI 1835 CTCGGG C^[CT]CG[GA]G
BalI 1972 TGGCCA TGG^CCA
BanII 135 GAGCCC G[GA]GC[CT]^C
BbsI 1466 GAAGAC GAAGAC(2/6)
BcgI 2185 CGACAGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 839 TGATCA T^GATCA
BglII 1143 AGATCT A^GATCT
BpuAI 1466 GAAGAC GAAGAC(2/6)
BsaHI 3184 GACGTC G[GA]^CG[CT]C
BsmBI 2339 CGTCTC CGTCTC(1/5)
BsmI 2031 GAATGC GAATGC(1/-1)
BsoBI 1835 CTCGGG C^[CT]CG[GA]G
Bsp1407I 448 TGTACA T^GTACA
BspLU11I 196 ACATGT A^CATGT
BsrGI 448 TGTACA T^GTACA
BssSI 3366 CACGAG CACGAG(-5/-1)
BstUI 1499 CGCG CG^CG
Bsu36I 1990 CCTGAGG CC^T[ACGT]AGG
DraII 1695 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eco47III 148 AGCGCT AGC^GCT
Eco81I 1990 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1695 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 2918 CCAAGG C^C[AT][AT]GG
FbaI 839 TGATCA T^GATCA
HaeII 148 AGCGCT [GA]GCGC^[CT]
HgaI 352 GCGTC GACGC(5/10)
Hin1I 3184 GACGTC G[GA]^CG[CT]C
KspI 1498 CCGCGG CCGC^GG
MluNI 1972 TGGCCA TGG^CCA
MscI 1972 TGGCCA TGG^CCA
MvnI 1499 CGCG CG^CG
PpuMI 1695 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1064 AACGTT AA^CGTT
PvuII 1968 CAGCTG CAG^CTG
SacII 1498 CCGCGG CCGC^GG
ScaI 2738 AGTACT AGT^ACT
SspBI 448 TGTACA T^GTACA
StyI 2918 CCAAGG C^C[AT][AT]GG
XcmI 970 CCAAGCCAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AseI 399 ATTAAT AT^TAAT
2925 ATTAAT
AsnI 399 ATTAAT AT^TAAT
2925 ATTAAT
Asp700I 427 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2031 GAATGCCTTC
BamHI 1995 GGATCC G^GATCC
2322 GGATCC
BanI 1986 GGTGCC G^G[CT][GA]CC
3313 GGTGCC
BsaI 2638 GAGACC GGTCTC(1/5)
2717 GAGACC
BseRI 965 CTCCTC GAGGAG(10/8)
2008 CTCCTC
CfoI 149 GCGC GCG^C
2091 GCGC
DsaI 1498 CCGCGG C^C[GA][CT]GG
1980 CCACGG
EaeI 1813 TGGCCG [CT]^GGCC[GA]
1972 TGGCCA
EcoT22I 1018 ATGCAT ATGCA^T
2573 ATGCAT
FauI 1497 CCCGC CCCGC(4/6)
1500 GCGGG
HhaI 149 GCGC GCG^C
2091 GCGC
HinP1I 149 GCGC G^CGC
2091 GCGC
HincII 1567 GTTGAC GT[CT]^[GA]AC
2779 GTTGAC
HindII 1567 GTTGAC GT[CT]^[GA]AC
2779 GTTGAC
MspA1I 1498 CCGCGG C[AC]G^C[GT]G
1968 CAGCTG
NdeI 2453 CATATG CA^TATG
2762 CATATG
NsiI 1018 ATGCAT ATGCA^T
2573 ATGCAT
PshBI 399 ATTAAT AT^TAAT
2925 ATTAAT
XmnI 427 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2031 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraIII EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII