Restriction map for
pF1KB4774
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbvCI BclI
BfaI BfrI BglI BglII BlnI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRV FbaI HpaI KpnI
MaeI MamI MluI MroI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 366 GACGTC GACGT^C
AccI 1288 GTCTAC GT^[AC][GT]AC
AviII 1952 TGCGCA TGC^GCA
BamHI 1391 GGATCC G^GATCC
BbeI 1167 GGCGCC GGCGC^C
BbrPI 1414 CACGTG CAC^GTG
BbsI 448 GTCTTC GAAGAC(2/6)
BciVI 849 GGATAC GTATCC(6/5)
BlpI 912 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 912 GCTCAGC GC^T[ACGT]AGC
BpuAI 448 GTCTTC GAAGAC(2/6)
BsaWI 2177 ACCGGA [AT]^CCGG[AT]
BssSI 637 CACGAG CACGAG(-5/-1)
BstAPI 1373 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 912 GCTCAGC GC^T[ACGT]AGC
DraIII 1039 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 178 GAAGAG CTCTTC(1/4)
EcoNI 93 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1243 GAATTC G^AATTC
EcoT14I 1823 CCAAGG C^C[AT][AT]GG
EcoT22I 808 ATGCAT ATGCA^T
FseI 58 GGCCGGCC GGCCGG^CC
FspI 1952 TGCGCA TGC^GCA
HincII 1624 GTCAAC GT[CT]^[GA]AC
HindII 1624 GTCAAC GT[CT]^[GA]AC
HindIII 943 AAGCTT A^AGCTT
KasI 1167 GGCGCC G^GCGCC
Ksp632I 178 GAAGAG CTCTTC(1/4)
MfeI 1260 CAATTG C^AATTG
MseI 1604 TTAA T^TAA
MunI 1260 CAATTG C^AATTG
NarI 1167 GGCGCC GG^CGCC
NsiI 808 ATGCAT ATGCA^T
PmaCI 1414 CACGTG CAC^GTG
PmlI 1414 CACGTG CAC^GTG
PpuMI 182 AGGACCT [GA]G^G[AT]CC[CT]
SfoI 1167 GGCGCC GGC^GCC
SmlI 1579 CTCAAG C^T[CT][GA]AG
StyI 1823 CCAAGG C^C[AT][AT]GG
TfiI 1778 GATTC G^A[AT]TC
Tru9I 1604 TTAA T^TAA
XcmI 376 CCAGAGCGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 1243 GAATTC [GA]^AATT[CT]
2155 AAATTC
AocI 186 CCTGAGG CC^T[ACGT]AGG
760 CCTCAGG
ApaI 191 GGGCCC GGGCC^C
582 GGGCCC
ApoI 1243 GAATTC [GA]^AATT[CT]
2155 AAATTC
Asp700I 269 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAACACATTC
BalI 897 TGGCCA TGG^CCA
1329 TGGCCA
BanI 1167 GGCGCC G^G[CT][GA]CC
1685 GGTGCC
BsaAI 562 TACGTG [CT]AC^GT[GA]
1414 CACGTG
BsaI 784 GAGACC GGTCTC(1/5)
970 GAGACC
BseRI 999 CTCCTC GAGGAG(10/8)
100 GAGGAG
BsgI 1713 GTGCAG GTGCAG(16/14)
2164 GTGCAG
BsiEI 57 CGGCCG CG[GA][CT]^CG
61 CGGCCG
BsmBI 1215 CGTCTC CGTCTC(1/5)
1587 CGTCTC
Bsu36I 186 CCTGAGG CC^T[ACGT]AGG
760 CCTCAGG
DsaI 573 CCGCGG C^C[GA][CT]GG
1665 CCGCGG
EagI 57 CGGCCG C^GGCCG
61 CGGCCG
EclXI 57 CGGCCG C^GGCCG
61 CGGCCG
Eco52I 57 CGGCCG C^GGCCG
61 CGGCCG
Eco81I 186 CCTGAGG CC^T[ACGT]AGG
760 CCTCAGG
HgaI 2138 GACGC GACGC(5/10)
51 GCGTC
KspI 573 CCGCGG CCGC^GG
1665 CCGCGG
MluNI 897 TGGCCA TGG^CCA
1329 TGGCCA
MscI 897 TGGCCA TGG^CCA
1329 TGGCCA
MslI 481 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2040 CATGCGCGTG
NaeI 59 GCCGGC GCC^GGC
353 GCCGGC
NgoMIV 59 GCCGGC G^CCGGC
353 GCCGGC
NspI 1034 ACATGC [GA]CATG^[CT]
2039 ACATGC
PflMI 636 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
958 CCAGCCTGTGG
SacII 573 CCGCGG CCGC^GG
1665 CCGCGG
TaqI 540 TCGA T^CGA
797 TCGA
TthHB8I 540 TCGA T^CGA
797 TCGA
Van91I 636 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
958 CCAGCCTGTGG
XmnI 269 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanII
BbvI BcgI BcnI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TseI Tsp45I Tsp509I TspRI XhoII
XmaCI XmaI