Restriction map for
pF1KB5105
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MseI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 368 ACGTGT A^C[GA][CT]GT
BalI 687 TGGCCA TGG^CCA
BamHI 1761 GGATCC G^GATCC
BanI 1135 GGTGCC G^G[CT][GA]CC
BanII 877 GAGCTC G[GA]GC[CT]^C
BbvCI 1063 GCTGAGG CCTCAGC(-5/-2)
BcnI 864 CCGGG CC^[GC]GG
BglI 1138 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 69 GGTCTC GGTCTC(1/5)
BseRI 359 GAGGAG GAGGAG(10/8)
BsgI 107 GTGCAG GTGCAG(16/14)
Bsp1407I 1476 TGTACA T^GTACA
BsrDI 534 CATTGC GCAATG(2/0)
BsrGI 1476 TGTACA T^GTACA
DraIII 1235 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 611 CCATGG C^C[GA][CT]GG
EaeI 687 TGGCCA [CT]^GGCC[GA]
EarI 918 GAAGAG CTCTTC(1/4)
EcoT22I 1829 ATGCAT ATGCA^T
FauI 103 GCGGG CCCGC(4/6)
HapII 864 CCGG C^CGG
HincII 681 GTTGAC GT[CT]^[GA]AC
HindII 681 GTTGAC GT[CT]^[GA]AC
HpaII 864 CCGG C^CGG
Ksp632I 918 GAAGAG CTCTTC(1/4)
MfeI 1344 CAATTG C^AATTG
MluNI 687 TGGCCA TGG^CCA
MscI 687 TGGCCA TGG^CCA
MslI 609 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 864 CCGG C^CGG
MunI 1344 CAATTG C^AATTG
NciI 864 CCGGG CC^[GC]GG
NcoI 611 CCATGG C^CATGG
NsiI 1829 ATGCAT ATGCA^T
NspI 1820 ACATGC [GA]CATG^[CT]
PflMI 1234 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 75 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 311 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 877 GAGCTC GAGCT^C
ScaI 1004 AGTACT AGT^ACT
SexAI 1236 ACCTGGT A^CC[AT]GGT
SspBI 1476 TGTACA T^GTACA
TfiI 989 GAATC G^A[AT]TC
Van91I 1234 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1054 TCTAGA T^CTAGA
XcmI 113 CCATGTATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 103 GCGG CCGC(-3/-1)
446 GCGG
AlwNI 74 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1587 CAGGTTCTG
Asp700I 1490 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1827 GAATGCATTC
AspHI 877 GAGCTC G[AT]GC[AT]^C
1714 GAGCAC
BbsI 1459 GAAGAC GAAGAC(2/6)
1816 GAAGAC
BpmI 746 CTGGAG CTGGAG(16/14)
1525 CTGGAG
BpuAI 1459 GAAGAC GAAGAC(2/6)
1816 GAAGAC
BsiHKAI 877 GAGCTC G[AT]GC[AT]^C
1714 GAGCAC
BsmFI 398 GGGAC GGGAC(10/14)
866 GGGAC
BsmI 1827 GAATGC GAATGC(1/-1)
1831 GCATTC
BstYI 228 AGATCC [GA]^GATC[CT]
1761 GGATCC
DraII 75 AGGACCT [GA]G^G[ACGT]CC[CT]
1067 AGGCCCT
EcoO109I 75 AGGACCT [GA]G^G[ACGT]CC[CT]
1067 AGGCCCT
EcoT14I 611 CCATGG C^C[AT][AT]GG
1379 CCTTGG
MflI 228 AGATCC [GA]^GATC[CT]
1761 GGATCC
PstI 1106 CTGCAG CTGCA^G
1447 CTGCAG
StyI 611 CCATGG C^C[AT][AT]GG
1379 CCTTGG
XhoII 228 AGATCC [GA]^GATC[CT]
1761 GGATCC
XmnI 1490 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1827 GAATGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII EcoRV Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I